Bemegride | CAS#64-65-3 | Central Nervous System Stimulant

MedKoo Cat#: 584144 | Name: Bemegride

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Bemegride is a central nervous system stimulant that is used to induce convulsions in experimental animals. It has also been used as a respiratory stimulant and in the treatment of barbiturate overdose.

Chemical Structure

Bemegride

CAS#64-65-3

Theoretical Analysis

MedKoo Cat#: 584144

Name: Bemegride

CAS#: 64-65-3

Chemical Formula: C8H13NO2

Exact Mass: 155.0946

Molecular Weight: 155.19

Elemental Analysis: C, 61.91; H, 8.44; N, 9.03; O, 20.62

Price and Availability

Size	Price	Availability	Quantity
250mg	USD 250.00	2 Weeks
1g	USD 550.00	2 Weeks

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Related CAS #

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Synonym

Bemegride; Antibarbi; Agipnon; Ahypnon; Eukraton; Malysol; Mikedimide; Zentraleptin

IUPAC/Chemical Name

2,6-Piperidinedione, 4-ethyl-4-methyl-

InChi Key

ORRZGUBHBVWWOP-UHFFFAOYSA-N

InChi Code

InChI=1S/C8H13NO2/c1-3-8(2)4-6(10)9-7(11)5-8/h3-5H2,1-2H3,(H,9,10,11)

SMILES Code

O=C(CC(C)(CC)C1)NC1=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

Bemegride is a central nervous system stimulant.

In vitro activity:

TBD

In vivo activity:

Changes in the energetic metabolism in the cortex were examined in bemegride-kindled rabbits. A reliable decrease in the ATP and an increase in the AMP contents in the cortical tissues as well as low ATP/AMP and energetic charge coefficients have been found after kindling the rabbits for 14 and 30-days as compared to the control groups of animals. Considerable disorders in the oxidative phosphorilation in the mitochondria of the cortical tissues have been determined after the succinate and glutamate oxidation: a decrease in the oxygen utilization rate at V3 active metabolic state, lowering the respiratory control coefficient and the disscciation between phosphorilation and oxidation. Those disorders were more expressed after glutamate oxidation as compared to a succinate one. In addition, it has been determined that oxygen utilization at V2 state was reliably decreased; both coefficient of energetic production and ATP-ase reserve activity lowered. Reference: Fiziol Zh. 2002;48(1):50-5. https://pubmed.ncbi.nlm.nih.gov/11928632/

	Solvent	mg/mL	mM
Solubility
	DMSO	31.0	199.76
	Ethanol	31.0	199.76

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 155.19 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Cherchenko AP, Todor IM. Zminy enerhetychnoho metabolizmu v tkanynakh mozku kroliv pry bemehrydovomu kindlinhu [Changes in energy metabolism in the brain of bemegride-kindled rabbits]. Fiziol Zh. 2002;48(1):50-5. Ukrainian. PMID: 11928632.

In vitro protocol:

TBD

In vivo protocol:

1: Katagai H, Yasui-Furukori N, Kikuchi A, Kaneko S. Bemegride lengthens seizure duration during electric convulsive therapy in a schizophrenic patient with increased seizure threshold. J ECT. 2009 Mar;25(1):61-3. doi: 10.1097/0b013e3181723725. PubMed PMID: 18955899. 2: Cherchenko AP, Todor IM. [Changes in energy metabolism in the brain of bemegride-kindled rabbits]. Fiziol Zh. 2002;48(1):50-5. Ukrainian. PubMed PMID: 11928632. 3: Jibiki I, Yamaguchi N, Matsuda H, Hisada K. Paradoxical enhancement of hypoperfusion single photon emission CT images in epileptic focus with bemegride activation. Observation from subtraction of 99mTc hexamethyl-propyleneamine oxime brain single photon emission CT images. Eur Neurol. 1992;32(3):146-50. PubMed PMID: 1592071. 4: Soto-Otero R, Mendez-Alvarez E, Sierra-Paredes G, Galan-Valiente J, Aguilar-Veiga E, Sierra-Marcuño G. Reversed-phase high-performance liquid chromatography method for the determination of bemegride in serum and brain tissue: pharmacokinetics and brain distribution of an intraperitoneal subconvulsive dose in rats. J Pharm Biomed Anal. 1991;9(2):177-82. PubMed PMID: 1873309. 5: Kawamura M, Murase K, Yasuhara Y, Mogami H, Tanada S, Hamamoto K, Hatakeyama T, Kimura H, Sakaki S. [Visualization of epileptic lesions using single photon emission computed tomography (SPECT) with N-isopropyl-p-(123I) iodoamphetamine after intravenous loading of bemegride--report of a case]. Nihon Igaku Hoshasen Gakkai Zasshi. 1989 Feb 25;49(2):199-205. Japanese. PubMed PMID: 2787907. 6: Mistry DK, Cottrell GA. Actions of steroids and bemegride on the GABAA receptor of mouse spinal neurones in culture. Exp Physiol. 1990 Mar;75(2):199-209. PubMed PMID: 2160252. 7: Arblaster CI, Cameron DW, Lavi Y, Laycock GM, Shulman A. The guinea-pig isolated atrium as a model system for the central actions of selected CNS stimulant and depressant drugs. Part 2. Mechanisms involved in the actions and interactions of the convulsant bemegride and its hypnotic homologue 3-methyl-3-n-butylglutarimide. Methods Find Exp Clin Pharmacol. 1988 Mar;10(3):177-211. PubMed PMID: 2455201. 8: Weber M, Huttin B, Gehin P, Vespignani H, Brichet B. [Changes in somatosensory evoked potentials during EEG activation by bemegride in man]. Rev Electroencephalogr Neurophysiol Clin. 1985 Jul;15(1):59-64. French. PubMed PMID: 4048612. 9: Bülau P, Fröscher W. [EEG activation using bemegride in the diagnosis of epilepsy. 2. Catamnestic studies]. EEG EMG Z Elektroenzephalogr Elektromyogr Verwandte Geb. 1984 Sep;15(3):132-7. German. PubMed PMID: 6435995. 10: Fröscher W, Bülau P. [EEG activation with bemegride in epilepsy diagnosis. 1: Literature review]. EEG EMG Z Elektroenzephalogr Elektromyogr Verwandte Geb. 1984 Jun;15(2):75-81. German. PubMed PMID: 6432516. 11: Scholfield CN. Antagonism of gamma-aminobutyric acid and muscimol by picrotoxin, bicuculline, strychnine, bemegride, leptazol, D-tubocurarine and theophylline in the isolated olfactory cortex. Naunyn Schmiedebergs Arch Pharmacol. 1982 Mar;318(4):274-80. PubMed PMID: 7078662. 12: Faingold CL, Hoffmann WE. Effects of bemegride on the sensory responses of neurons in the hippocampus and brain stem reticular formation. Electroencephalogr Clin Neurophysiol. 1981 Oct;52(4):316-27. PubMed PMID: 6169508. 13: Shearman G, Lal H. Discriminative stimulus properties of pentylenetetrazol and bemegride: some generalization and antagonism tests. Psychopharmacology (Berl). 1979 Sep;64(3):315-9. PubMed PMID: 41279. 14: Micheletti M, Laroye M, Coquillat G, Micheletti G, Kurtz D. [Semiological comparison of spontaneous and bemegride-induced epileptic seizures (author's transl)]. Rev Electroencephalogr Neurophysiol Clin. 1979 Jul-Sep;9(3):241-9. French. PubMed PMID: 523736. 15: Järbe TU. Alcohol-discrimination in gerbils: interactions with bemegride, DH-524, Amphetamine, and delta9-THC. Arch Int Pharmacodyn Ther. 1977 May;227(1):118-29. PubMed PMID: 901060. 16: Järbe TU, Johansson JO. Pentobarbital, diazepam and bemegride: their effects on open-field behavior in the gerbil (Meriones unguiculatus). Arch Int Pharmacodyn Ther. 1977 Jan;225(1):88-97. PubMed PMID: 849067. 17: Buchbauer G. [Phytopharmacological study on proadiphene, amiphenazole and bemegride in association with phenobarbitone (author's transl)]. Arzneimittelforschung. 1977;27(5):976-8. German. PubMed PMID: 577883. 18: Jårbe. Methaqualone discrimination in gerbils: interactions with bemegride and imidazoline (DH-524). Med Biol. 1976 Dec;54(5):364-8. PubMed PMID: 162743. 19: Johansson JO, Järbe TU. Diazepam as a discriminative cue: its antagonism by bemegride. Eur J Pharmacol. 1975 Feb;30(2):372-5. PubMed PMID: 1126370. 20: Aleksandrov AA, Lenkov DN. [The influence of bemegride on frog spinal cord root potentials]. Neirofiziologiia. 1975;7(5):527-31. Russian. PubMed PMID: 1207835.