JNK-IN-7 | CAS# 1408064-71-0 | Novel potent JNK inhibitor

MedKoo Cat#: 581024 | Name: JNK-IN-7

Description:

WARNING: This product is for research use only, not for human or veterinary use.

JNK-IN-7 is a relatively selective JNKs inhibitor(IC50= 1.54/1.99/0.75 for JNK1/2/3); also bound to IRAK1, PIK3C3, PIP5K3 and PIP4K2C.

Chemical Structure

JNK-IN-7

CAS#1408064-71-0

Theoretical Analysis

MedKoo Cat#: 581024

Name: JNK-IN-7

CAS#: 1408064-71-0

Chemical Formula: C28H27N7O2

Exact Mass: 493.2226

Molecular Weight: 493.57

Elemental Analysis: C, 68.14; H, 5.51; N, 19.87; O, 6.48

Price and Availability

Size	Price	Availability	Quantity
100mg	USD 1,650.00	2 Weeks
1g	USD 4,650.00	2 Weeks

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Synonym

JNK-IN-7; JNK inhibitor;JNKIN7; JNK IN 7; JNK-IN-7.

IUPAC/Chemical Name

3-[[(E)-4-(Dimethylamino)but-2-enoyl]amino]-N-[4-[(4-pyridin-3-ylpyrimidin-2-yl)amino]phenyl]benzamide

InChi Key

RADRIIWGHYFWPP-WEVVVXLNSA-N

InChi Code

InChI=1S/C28H27N7O2/c1-35(2)17-5-9-26(36)31-24-8-3-6-20(18-24)27(37)32-22-10-12-23(13-11-22)33-28-30-16-14-25(34-28)21-7-4-15-29-19-21/h3-16,18-19H,17H2,1-2H3,(H,31,36)(H,32,37)(H,30,33,34)/b9-5+

SMILES Code

O=C(NC1=CC=C(NC2=NC=CC(C3=CC=CN=C3)=N2)C=C1)C4=CC=CC(NC(/C=C/CN(C)C)=O)=C4

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

JNK-IN-7 is a potent JNK inhibitor with IC50 of 1.5, 2 and 0.7 nM for JNK1, JNK2 and JNK3, respectively.

In vitro activity:

As shown in Fig. 5B, co-incubation of JNK signaling inhibitor (JNK-IN-7) significantly suppressed the down-regulation of the DMT1 mRNA expression (P < 0.05), while TNF alone or co-incubation with NF-κB and caspase-3/8 inhibitors significantly down-regulated DMT1 mRNA expression compared to controls (P < 0.05). Thus, these results prove that TNF could down-regulate DMT1 expression through the JNK pathway. Reference: Sci Rep. 2015 Nov 17;5:16344. https://pubmed.ncbi.nlm.nih.gov/26572590/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMF	10.0	20.26
	DMSO	31.1	63.03
	DMSO:PBS (pH 7.2) (1:2)	0.3	0.61

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 493.57 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Wu W, Song Y, He C, Liu C, Wu R, Fang L, Cong Y, Miao Y, Liu Z. Divalent metal-ion transporter 1 is decreased in intestinal epithelial cells and contributes to the anemia in inflammatory bowel disease. Sci Rep. 2015 Nov 17;5:16344. doi: 10.1038/srep16344. PMID: 26572590; PMCID: PMC4648093. 2. Zhang T, Inesta-Vaquera F, Niepel M, Zhang J, Ficarro SB, Machleidt T, Xie T, Marto JA, Kim N, Sim T, Laughlin JD, Park H, LoGrasso PV, Patricelli M, Nomanbhoy TK, Sorger PK, Alessi DR, Gray NS. Discovery of potent and selective covalent inhibitors of JNK. Chem Biol. 2012 Jan 27;19(1):140-54. doi: 10.1016/j.chembiol.2011.11.010. PMID: 22284361; PMCID: PMC3270411.

In vitro protocol:

In vivo protocol:

TBD

1: Wang M, Xu Y, Liu J, Ye J, Yuan W, Jiang H, Wang Z, Jiang H, Wan J. Recent Insights into the Biological Functions of Sestrins in Health and Disease. Cell Physiol Biochem. 2017 Oct 19;43(5):1731-1741. doi: 10.1159/000484060. [Epub ahead of print] PubMed PMID: 29050006. 2: Zhang Y, Deng X, Lei T, Yu C, Wang Y, Zhao G, Luo X, Tang K, Quan Z, Jiang D. Capsaicin inhibits proliferation and induces apoptosis in osteosarcoma cell lines via the mitogen activated protein kinase pathway. Oncol Rep. 2017 Sep 18. doi: 10.3892/or.2017.5960. [Epub ahead of print] PubMed PMID: 29048662. 3: Min SK, Jung SY, Kang HK, Park SA, Lee JH, Kim MJ, Min BM. Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells. Int J Oncol. 2017 Sep 14. doi: 10.3892/ijo.2017.4124. [Epub ahead of print] PubMed PMID: 29048658. 4: Shlyakhtina Y, Pavet V, Gronemeyer H. Dual role of DR5 in death and survival signaling leads to TRAIL resistance in cancer cells. Cell Death Dis. 2017 Aug 31;8(8):e3025. doi: 10.1038/cddis.2017.423. PubMed PMID: 29048428. 5: Cholujova D, Bujnakova Z, Dutkova E, Hideshima T, Groen RW, Mitsiades CS, Richardson PG, Dorfman DM, Balaz P, Anderson KC, Jakubikova J. Realgar nanoparticles versus ATO arsenic compounds induce in vitro and in vivo activity against multiple myeloma. Br J Haematol. 2017 Oct 19. doi: 10.1111/bjh.14974. [Epub ahead of print] PubMed PMID: 29048129. 6: Min K, Lawan A, Bennett AM. Loss of MKP-5 promotes myofiber survival by activating STAT3/Bcl-2 signaling during regenerative myogenesis. Skelet Muscle. 2017 Oct 18;7(1):21. doi: 10.1186/s13395-017-0137-7. PubMed PMID: 29047406. 7: Guo J, Li W, Wu Y, Jing X, Huang J, Zhang J, Xiang W, Ren R, Lv Z, Xiao J, Guo F. Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR. Front Pharmacol. 2017 Oct 4;8:693. doi: 10.3389/fphar.2017.00693. eCollection 2017. PubMed PMID: 29046637; PubMed Central PMCID: PMC5632684. 8: Chen W, Guo Y, Yang W, Chen L, Ren D, Wu C, He B, Zheng P, Tong W. Phosphorylation of Connexin 43 Induced by Traumatic Brain Injury Promotes Exosome Release. J Neurophysiol. 2017 Oct 18:jn.00654.2017. doi: 10.1152/jn.00654.2017. [Epub ahead of print] PubMed PMID: 29046426. 9: Izawa T, Hutami IR, Tanaka E. Potential role of rebamipide in osteoclast differentiation and mandibular condylar cartilage homeostasis. Curr Rheumatol Rev. 2017 Oct 17. doi: 10.2174/1573397113666171017113441. [Epub ahead of print] PubMed PMID: 29046162. 10: Singh P, Chowdhuri DK. Modulation of sestrin confers protection to Cr(VI) induced neuronal cell death in Drosophila melanogaster. Chemosphere. 2017 Oct 7;191:302-314. doi: 10.1016/j.chemosphere.2017.10.037. [Epub ahead of print] PubMed PMID: 29045932. 11: Hwang IH, Oh SY, Jang HJ, Jo E, Joo JC, Lee KB, Yoo HS, Lee MY, Park SJ, Jang IS. Cordycepin promotes apoptosis in renal carcinoma cells by activating the MKK7-JNK signaling pathway through inhibition of c-FLIPL expression. PLoS One. 2017 Oct 18;12(10):e0186489. doi: 10.1371/journal.pone.0186489. eCollection 2017. PubMed PMID: 29045468. 12: Wang S, Sui S, Liu Z, Peng C, Liu J, Luo D, Fan X, Liu C, Lu WY. Protective roles of hepatic gamma-aminobutyric acid signaling in acute ethanol exposure-induced liver injury. J Appl Toxicol. 2017 Oct 17. doi: 10.1002/jat.3544. [Epub ahead of print] PubMed PMID: 29044621. 13: Chen Y, Guo F, Ru Z, Kong H, Sun H, Yu H, Yang W, Zhang Q, Zhou M. Nuclear factor-κB signaling negatively regulates high glucose-induced vascular endothelial cell damage downstream of the extracellular signal-regulated kinase/c-Jun N-terminal kinase pathway. Exp Ther Med. 2017 Oct;14(4):3851-3855. doi: 10.3892/etm.2017.4999. Epub 2017 Aug 22. PubMed PMID: 29042991; PubMed Central PMCID: PMC5639326. 14: Luo D, Guo Y, Cheng Y, Zhao J, Wang Y, Rong J. Natural product celastrol suppressed macrophage M1 polarization against inflammation in diet-induced obese mice via regulating Nrf2/HO-1, MAP kinase and NF-κB pathways. Aging (Albany NY). 2017 Oct 16. doi: 10.18632/aging.101302. [Epub ahead of print] PubMed PMID: 29040966. 15: Lim W, Yang C, Jeong M, Bazer FW, Song G. Coumestrol induces mitochondrial dysfunction by stimulating ROS production and calcium ion influx into mitochondria in human placental choriocarcinoma cells. Mol Hum Reprod. 2017 Oct 11. doi: 10.1093/molehr/gax052. [Epub ahead of print] PubMed PMID: 29040664. 16: Sun Z, Li G, Tong T, Chen J. Micheliolide suppresses LPS-induced neuroinflammatory responses. PLoS One. 2017 Oct 17;12(10):e0186592. doi: 10.1371/journal.pone.0186592. eCollection 2017. PubMed PMID: 29040306. 17: Zhai T, Hei Z, Ma Q, Liang H, Xu Y, Zhang Y, Jin L, Han C, Wang J. Shikonin induces apoptosis and G0/G1 phase arrest of gallbladder cancer cells via the JNK signaling pathway. Oncol Rep. 2017 Oct 17. doi: 10.3892/or.2017.6038. [Epub ahead of print] PubMed PMID: 29039581. 18: Liang K, Qian WH, Zong J. 3,3' Diindolylmethane attenuates cardiomyocyte hypoxia by modulating autophagy in H9c2 cells. Mol Med Rep. 2017 Oct 13. doi: 10.3892/mmr.2017.7788. [Epub ahead of print] PubMed PMID: 29039568. 19: Zhao T, Bai J, Zou Q, Chen F, Xie Y. Insulin in combination with cisplatin induces the apoptosis of ovarian cancer cells via p53 and JNK activation. Mol Med Rep. 2017 Oct 10. doi: 10.3892/mmr.2017.7752. [Epub ahead of print] PubMed PMID: 29039558. 20: Fang W, Cai SX, Wang CL, Sun XX, Li K, Yan XW, Sun YB, Sun XZ, Gu CK, Dai MY, Wang HM, Zhou Z. Modulation of mitogen activated protein kinase attenuates sepsis induced acute lung injury in acute respiratory distress syndrome rats. Mol Med Rep. 2017 Oct 17. doi: 10.3892/mmr.2017.7811. [Epub ahead of print] PubMed PMID: 29039541.