OUL35 | CAS#6336-34-1 | ARTD10 inhibitor

MedKoo Cat#: 561162 | Name: OUL35

Description:

WARNING: This product is for research use only, not for human or veterinary use.

OUL35, also known as NSC39047, is a selective PARP-10 inhibitor, and small-molecule ARTD10 inhibitor. OUL35 has been shown to rescue cells from ARTD10-induced cell death.

Chemical Structure

OUL35

CAS#6336-34-1

Theoretical Analysis

MedKoo Cat#: 561162

Name: OUL35

CAS#: 6336-34-1

Chemical Formula: C14H12N2O3

Exact Mass: 256.0848

Molecular Weight: 256.26

Elemental Analysis: C, 65.62; H, 4.72; N, 10.93; O, 18.73

Price and Availability

Size	Price	Availability
50mg	USD 250.00	2 Weeks
100mg	USD 450.00	2 Weeks
200mg	USD 750.00	2 Weeks
500mg	USD 1,450.00	2 Weeks
1g	USD 2,450.00	2 Weeks
2g	USD 4,650.00	2 Weeks
5g	USD 6,950.00	2 Weeks

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Related CAS #

No Data

Synonym

OUL35; OUL 35; OUL-35; NSC39047; NSC 39047; NSC-39047

IUPAC/Chemical Name

4-(4-Carbamoylphenoxy)benzamide

InChi Key

XZRCQWLPMXFGHE-UHFFFAOYSA-N

InChi Code

InChI=1S/C14H12N2O3/c15-13(17)9-1-5-11(6-2-9)19-12-7-3-10(4-8-12)14(16)18/h1-8H,(H2,15,17)(H2,16,18)

SMILES Code

O=C(N)C1=CC=C(OC2=CC=C(C(N)=O)C=C2)C=C1

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

OUL35 (NSC39047) is a potent and selective inhibitor of ARTD10 (PARP-10), with an IC50 of 329 nM.

In vitro activity:

The treatment of HeLa cells overexpressing ARTD10 with OUL35 resulted in a complete rescue of cell proliferation. Notably, the inactive analogs OUL77 and OUL78 were ineffective and did not facilitate colony formation (Figures 3A and 3B). Together, these findings provide evidence that OUL35 enters cells and inhibits the catalytic activity of ARTD10 in a dose-dependent manner with an IC50 of 1.35 μM (Figure 3C). To further substantiate this, this study performed cellular thermal shift assays (CETSA) showing that endogenous ARTD10 in U2OS cells is stabilized by OUL35 (Figure 3D). Reference: Cell Chem Biol. 2016 Oct 20;23(10):1251-1260. https://pubmed.ncbi.nlm.nih.gov/27667561/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMF	5.0	19.51
	DMSO	46.3	180.75
	DMSO:PBS (pH 7.2) (1:4)	0.2	0.78

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 256.26 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Tian Y, Korn P, Tripathi P, Komnig D, Wiemuth D, Nikouee A, Classen A, Bolm C, Falkenburger BH, Lüscher B, Gründer S. The mono-ADP-ribosyltransferase ARTD10 regulates the voltage-gated K+ channel Kv1.1 through protein kinase C delta. BMC Biol. 2020 Oct 15;18(1):143. doi: 10.1186/s12915-020-00878-1. PMID: 33059680; PMCID: PMC7558731. 2. Venkannagari H, Verheugd P, Koivunen J, Haikarainen T, Obaji E, Ashok Y, Narwal M, Pihlajaniemi T, Lüscher B, Lehtiö L. Small-Molecule Chemical Probe Rescues Cells from Mono-ADP-Ribosyltransferase ARTD10/PARP10-Induced Apoptosis and Sensitizes Cancer Cells to DNA Damage. Cell Chem Biol. 2016 Oct 20;23(10):1251-1260. doi: 10.1016/j.chembiol.2016.08.012. Epub 2016 Sep 22. PMID: 27667561.

In vitro protocol:

In vivo protocol:

TBD

1: Venkannagari H, Verheugd P, Koivunen J, Haikarainen T, Obaji E, Ashok Y, Narwal M, Pihlajaniemi T, Lüscher B, Lehtiö L. Small-Molecule Chemical Probe Rescues Cells from Mono-ADP-Ribosyltransferase ARTD10/PARP10-Induced Apoptosis and Sensitizes Cancer Cells to DNA Damage. Cell Chem Biol. 2016 Oct 20;23(10):1251-1260. doi: 10.1016/j.chembiol.2016.08.012. Epub 2016 Sep 22. PubMed PMID: 27667561.