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MedKoo Cat#: 525397 | Name: DPO
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

DPO is a quorum sensing (QS) autoinducer, binding to and activating a transcription factor, VqmA.

Chemical Structure

DPO
DPO
CAS#60187-00-0

Theoretical Analysis

MedKoo Cat#: 525397

Name: DPO

CAS#: 60187-00-0

Chemical Formula: C6H8N2O

Exact Mass: 124.0637

Molecular Weight: 124.14

Elemental Analysis: C, 58.05; H, 6.50; N, 22.57; O, 12.89

Price and Availability

Size Price Availability Quantity
250mg USD 250.00 2 Weeks
1g USD 550.00 2 Weeks
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Related CAS #
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Synonym
DPO; 3,5-Dimethylpyrazinol
IUPAC/Chemical Name
3,5-Dimethylpyrazin-2-ol
InChi Key
AJYKJVCIKQEVCF-UHFFFAOYSA-N
InChi Code
InChI=1S/C6H8N2O/c1-4-3-7-6(9)5(2)8-4/h3H,1-2H3,(H,7,9)
SMILES Code
OC1=NC=C(C)N=C1C
Appearance
Purity
>97% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
DPO is a quorum sensing (QS) autoinducer, binding to and activating a transcription factor, VqmA.
In vitro activity:
The DPO-VqmA complex activates transcription of vqmR, encoding the VqmR small RNA, which represses genes required for biofilm formation and virulence factor production. Here, this study shows that VqmA is soluble and properly folded and activates basal-level transcription of its target vqmR in the absence of DPO. VqmA transcriptional activity is increased in response to increasing concentrations of DPO, allowing VqmA to drive the V. cholerae quorum-sensing transition at high cell densities. Reference: J Biol Chem. 2020 Mar 6;295(10):2916-2931. https://pubmed.ncbi.nlm.nih.gov/31964715/
In vivo activity:
TBD

Preparing Stock Solutions

The following data is based on the product molecular weight 124.14 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Huang X, Duddy OP, Silpe JE, Paczkowski JE, Cong J, Henke BR, Bassler BL. Mechanism underlying autoinducer recognition in the Vibrio cholerae DPO-VqmA quorum-sensing pathway. J Biol Chem. 2020 Mar 6;295(10):2916-2931. doi: 10.1074/jbc.RA119.012104. Epub 2020 Jan 21. PMID: 31964715; PMCID: PMC7062168.
In vitro protocol:
1. Huang X, Duddy OP, Silpe JE, Paczkowski JE, Cong J, Henke BR, Bassler BL. Mechanism underlying autoinducer recognition in the Vibrio cholerae DPO-VqmA quorum-sensing pathway. J Biol Chem. 2020 Mar 6;295(10):2916-2931. doi: 10.1074/jbc.RA119.012104. Epub 2020 Jan 21. PMID: 31964715; PMCID: PMC7062168.
In vivo protocol:
TBD
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