Saccharin 1-methylimidazole, also known as SMI, is a chemical activator commonly used in solid-phase synthesis of DNA and RNA oligonucleotides. Saccharin 1-methylimidazole has been used to synthesize single-stranded DNA oligonucleotides that are resistant to nuclease digestion.
MedKoo Cat#: 526295
Name: Saccharin 1-methylimidazole
CAS#: 482333-74-4 (1-methylimidazole)
Chemical Formula: C11H11N3O3S
Exact Mass: 265.0521
Molecular Weight: 265.29
Elemental Analysis: C, 49.80; H, 4.18; N, 15.84; O, 18.09; S, 12.09
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 52.7 | 198.54 | |
| Water | 53.0 | 199.78 | |
| Ethanol | 10.0 | 37.69 |
The following data is based on the product molecular weight 265.29 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
