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MedKoo Cat#: 526264 | Name: Suprafenacine
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Suprafenacine is a novel cell permeable selective inhibitor of cancer cell proliferation, binding microtubules at the colchicine-binding site and inhibiting polymerization, leading to G2/M cell cycle arrest and cell death via a mitochondria-mediated apoptotic pathway.

Chemical Structure

Suprafenacine
Suprafenacine
CAS#1477482-50-0

Theoretical Analysis

MedKoo Cat#: 526264

Name: Suprafenacine

CAS#: 1477482-50-0

Chemical Formula: C16H18N4O

Exact Mass: 282.1481

Molecular Weight: 282.35

Elemental Analysis: C, 68.06; H, 6.43; N, 19.84; O, 5.67

Price and Availability

Size Price Availability Quantity
5mg USD 400.00
25mg USD 850.00
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Related CAS #
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Synonym
Suprafenacine
IUPAC/Chemical Name
N-[(E)-(4-Methylphenyl)methylideneamino]-4,5,6,7-tetrahydro-1H-indazole-3-carboxamide
InChi Key
WKKLZIQHOXPLLZ-LICLKQGHSA-N
InChi Code
InChI=1S/C16H18N4O/c1-11-6-8-12(9-7-11)10-17-20-16(21)15-13-4-2-3-5-14(13)18-19-15/h6-10H,2-5H2,1H3,(H,18,19)(H,20,21)/b17-10+
SMILES Code
O=C(C1=NNC2=C1CCCC2)N/N=C/C3=CC=C(C)C=C3
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
The biological target of suprafenacine is microtubules. It binds to the colchicine-binding site on microtubules, leading to destabilization and inhibition of microtubule polymerization.
In vitro activity:
In this in vitro study, Suprafenacine (SRF), a novel tubulin-destabilizing molecule, was identified through in silico screening. SRF binds to the colchicine-binding site on microtubules, inhibiting polymerization and leading to G2/M cell cycle arrest and subsequent cell death via a mitochondria-mediated apoptotic pathway. Notably, SRF demonstrated selectivity in inhibiting cancer cell proliferation and efficacy against drug-resistant cancer cells. Reference: Choi BH, Chattopadhaya S, Thanh le N, Feng L, Nguyen QT, Lim CB, Harikishore A, Nanga RP, Bharatham N, Zhao Y, Liu X, Yoon HS. Suprafenacine, an indazole-hydrazide agent, targets cancer cells through microtubule destabilization. PLoS One. 2014 Oct 29;9(10):e110955.
In vivo activity:
To Be Determined
Solvent mg/mL mM
Solubility
DMSO 10.0 35.40
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 282.35 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
Choi BH, Chattopadhaya S, Thanh le N, Feng L, Nguyen QT, Lim CB, Harikishore A, Nanga RP, Bharatham N, Zhao Y, Liu X, Yoon HS. Suprafenacine, an indazole-hydrazide agent, targets cancer cells through microtubule destabilization. PLoS One. 2014 Oct 29;9(10):e110955. doi: 10.1371/journal.pone.0110955. Erratum in: PLoS One. 2018 Jul 18;13(7):e0201149. doi: 10.1371/journal.pone.0201149. PMID: 25354194; PMCID: PMC4212991.
In vitro protocol:
Choi BH, Chattopadhaya S, Thanh le N, Feng L, Nguyen QT, Lim CB, Harikishore A, Nanga RP, Bharatham N, Zhao Y, Liu X, Yoon HS. Suprafenacine, an indazole-hydrazide agent, targets cancer cells through microtubule destabilization. PLoS One. 2014 Oct 29;9(10):e110955. doi: 10.1371/journal.pone.0110955. Erratum in: PLoS One. 2018 Jul 18;13(7):e0201149. doi: 10.1371/journal.pone.0201149. PMID: 25354194; PMCID: PMC4212991.
In vivo protocol:
To Be Determined
1: Choi BH, Chattopadhaya S, Thanh le N, Feng L, Nguyen QT, Lim CB, Harikishore A, Nanga RP, Bharatham N, Zhao Y, Liu X, Yoon HS. Suprafenacine, an indazole-hydrazide agent, targets cancer cells through microtubule destabilization. PLoS One. 2014 Oct 29;9(10):e110955. doi: 10.1371/journal.pone.0110955. eCollection 2014. PubMed PMID: 25354194; PubMed Central PMCID: PMC4212991.