MDK-5561, also known as CLK-IN-T3, is a novel highly selective, stable CLK inhibitor with high specificity to CLK1-3 protein isoforms. CLK-IN-T3 (MDK-5561) has CAS#2109805-56-1. The last 4 digits of the CAS# is used for name for the convenience of scientific communications. T3-CLK is a pan-CLK inhibitor, T3-CLK as well as the negative control T3-CLK-N have been developed in collaboration with Takeda 3 (5). T3-CLK is a potent inhibitor of CLK1, CLK2 and CLK3 with IC50 of 0.67/15/110 nM, respectively. T3-CLK is >30 fold selective against the closest off targets DYRK1A and DYRK1B with an IC50 of 260 nM and 230 nM, respectively in cellular assays. T3-CLK is non-toxic in cells and inhibits the phosphorylation of SRSF in HeLa cells in a time and dose dependent manner. (https://www.thesgc.org/chemical-probes/T3-CLK)
MedKoo Cat#: 525696
Name: CLK-IN-T3 HCl
CAS#: CLK-IN-T3 HCl
Chemical Formula: C28H31ClN6O2
Exact Mass: 0.0000
Molecular Weight: 519.05
Elemental Analysis: C, 64.79; H, 6.02; Cl, 6.83; N, 16.19; O, 6.16
The following data is based on the product molecular weight 519.05 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
