HC-056456 | CatSper channel modulator | CAS#7733-96-2

MedKoo Cat#: 531948 | Name: HC-056456

Description:

WARNING: This product is for research use only, not for human or veterinary use.

HC-056456 is a CatSper channel modulator.

Chemical Structure

HC-056456

CAS#7733-96-2

Theoretical Analysis

MedKoo Cat#: 531948

Name: HC-056456

CAS#: 7733-96-2

Chemical Formula: C12H6N2O4S2

Exact Mass: 305.9769

Molecular Weight: 306.31

Elemental Analysis: C, 47.05; H, 1.97; N, 9.15; O, 20.89; S, 20.93

Price and Availability

Size	Price	Availability
10mg	USD 120.00	Ready to ship
25mg	USD 190.00	Ready to ship
50mg	USD 350.00	Ready to ship
100mg	USD 550.00	Ready to ship
200mg	USD 950.00	Ready to ship
500mg	USD 1,950.00	Ready to ship
1g	USD 3,450.00	Ready to ship

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Related CAS #

No Data

Synonym

HC-056456; HC 056456; HC056456.

IUPAC/Chemical Name

bis[(thiophen-2-yl)carbonyl]-1,2,5-oxadiazol-2-ium-2-olate

InChi Key

RUQGCDMXFBOTMW-UHFFFAOYSA-N

InChi Code

InChI=1S/C12H6N2O4S2/c15-11(7-3-1-5-19-7)9-10(14(17)18-13-9)12(16)8-4-2-6-20-8/h1-6H

SMILES Code

[O-][N+]1=C(C(C2=CC=CS2)=O)C(C(C3=CC=CS3)=O)=NO1

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Certificate of Analysis

View CoA: current batch, Lot#A8T09K07

QC Data

View QC data: current batch, Lot#A8T09K07

Safety Data Sheet (SDS)

View Safety Data Sheet (SDS)

Instruction

View handling instruction

Biological target:

HC-056456 is a blocker of CatSper channels.

In vitro activity:

N/A

In vivo activity:

The CatSper channel is the predominant route for evoked Ca2+ entry into intact mouse sperm. Compound HC-056456 was identified as a potential inhibitor of CatSper in a chemical-library screenThe efficacy of HC-056456 to inhibit evoked Ca2+ entry was examined. In Figs. 3A–C, fura-2 reported the spatially-averaged [Ca2+]i of sperm during challenge with a paired-stimulus protocol. Medium K8.6 evoked averaged rates of rise of 8.7 and 6.6 nM Δ[Ca2+]i s−1 during the first and second stimuli when no inhibitor was present. When 3 µM HC-056456 was applied after the first stimulus, the averaged rate decreased from ∼7.5 to ∼1.6 nM Δ[Ca2+]i s−1. When 10 µM HC-056456 was applied after the first stimulus, the averaged rate decreased more strongly, from ∼7.0 to ∼0.7 nM Δ[Ca2+]i s−1. Hence HC-056456 is an effective inhibitor of the CatSper Ca2+ channel activity that is evoked by alkaline depolarization. Fig. 3D shows that at 10 µM the HC-056456 also strongly decreased the rise in [Na+]i that was evoked by removal of external Ca2+. The rates of rise before and during exposure to HC-056456 were ∼0.03 and ∼0.01 ΔRSBFI min−1. Reference: PLoS One. 2009; 4(8): e6844. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729922/

	Solvent	mg/mL	mM
Solubility
	DMSO	150.0	489.70

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 306.31 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

N/A

In vitro protocol:

N/A

In vivo protocol:

1. Carlson AE, Burnett LA, del Camino D, Quill TA, Hille B, Chong JA, Moran MM, Babcock DF. Pharmacological targeting of native CatSper channels reveals a required role in maintenance of sperm hyperactivation. PLoS One. 2009 Aug 31;4(8):e6844. doi: 10.1371/journal.pone.0006844. PMID: 19718436; PMCID: PMC2729922.

1: Carlson AE, Burnett LA, del Camino D, Quill TA, Hille B, Chong JA, Moran MM, Babcock DF. Pharmacological targeting of native CatSper channels reveals a required role in maintenance of sperm hyperactivation. PLoS One. 2009 Aug 31;4(8):e6844. doi: 10.1371/journal.pone.0006844. PubMed PMID: 19718436; PubMed Central PMCID: PMC2729922.