Pyridoxine | CAS#65-23-6 | 58-56-0 | vitamin B6

MedKoo Cat#: 329469 | Name: Pyridoxine free base

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Pyridoxine (also called pyridoxol, not to be confused with pyridoxal) is one form of vitamin B6. Its hydrochloride salt, pyridoxine hydrochloride, is used as a vitamin B6 dietary supplement.

Chemical Structure

Pyridoxine free base

CAS#65-23-6 (free base)

Theoretical Analysis

MedKoo Cat#: 329469

Name: Pyridoxine free base

CAS#: 65-23-6 (free base)

Chemical Formula: C8H11NO3

Exact Mass: 169.0739

Molecular Weight: 169.18

Elemental Analysis: C, 56.80; H, 6.55; N, 8.28; O, 28.37

Price and Availability

Size	Price	Availability	Quantity
1g	USD 60.00
2g	USD 90.00
5g	USD 150.00
10g	USD 250.00
20g	USD 350.00
50g	USD 450.00
100g	USD 650.00

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Related CAS #

65-23-6 (free base) 58-56-0 (hydrochloride) 27280-85-9 (oxoglurate)

Synonym

Pyridoxine; pyridoxol. vitamin B6.

IUPAC/Chemical Name

4,5-Bis(hydroxymethyl)-2-methylpyridin-3-ol

InChi Key

LXNHXLLTXMVWPM-UHFFFAOYSA-N

InChi Code

InChI=1S/C8H11NO3/c1-5-8(12)7(4-11)6(3-10)2-9-5/h2,10-12H,3-4H2,1H3

SMILES Code

OC1=C(CO)C(CO)=CN=C1C

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO and DMF

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Related CAS# CAS#65-23-6 (Pyridoxine free base) CAS#58-56-0 (Pyridoxine hydrochloride)

Product Data

Product Data

Certificate of Analysis

View CoA: current batch, Lot#C8M06C27

Safety Data Sheet (SDS)

View Safety Data Sheet (SDS)

Instruction

View handling instruction

Biological target:

Pyridoxine is a water-soluble vitamin, and its active form, pyridoxal phosphate (PLP), serves as a coenzyme in several enzyme-catalyzed reactions. Pyridoxine primarily targets enzymes that rely on PLP as a cofactor to facilitate specific biochemical reactions.

In vitro activity:

In this cell model of Alzheimer's disease, pyridoxine exerted a protective potential against Alzheimer's disease, attenuated ROS levels, decreased the expressions of cytoplasmic Nrf2, and upregulated whole-cell HO-1 expression. Reference: Cell Mol Biol (Noisy-le-grand). 2018 Jul 30;64(10):119-124. https://pubmed.ncbi.nlm.nih.gov/30084803/

In vivo activity:

Pyridoxine may be an effective adjuvant agent for the prevention of linezolid toxicity in rat models. Linezolid plus pyridoxine treatment caused a significant decrease in malondialdehyde levels and superoxide dismutase, catalase, and glutathione peroxidase enzyme activities compared to the linezolid only group (P < .001, P < .01, P < .001, and P < .01, respectively). Reference: Turk Arch Pediatr. 2023 May;58(3):298-301. https://pubmed.ncbi.nlm.nih.gov/37144263/

	Solvent	mg/mL	mM
Solubility
	DMF	0.3	1.48
	DMSO	10.0	59.11

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 169.18 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Li C, Wang R, Hu C, Wang H, Ma Q, Chen S, He Y. Pyridoxine exerts antioxidant effects in cell model of Alzheimer's disease via the Nrf-2/HO-1 pathway. Cell Mol Biol (Noisy-le-grand). 2018 Jul 30;64(10):119-124. PMID: 30084803. 2. Vrolijk MF, Opperhuizen A, Jansen EHJM, Hageman GJ, Bast A, Haenen GRMM. The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function. Toxicol In Vitro. 2017 Oct;44:206-212. doi: 10.1016/j.tiv.2017.07.009. Epub 2017 Jul 14. PMID: 28716455. 3. Kendir-Demirkol Y, Jenny LA, Demirkol A, Özen M, Ayata A, Canatan D. The Protective Effects of Pyridoxine on Linezolid-Induced Hematological Toxicity, Hepatotoxicity, and Oxidative Stress in Rats. Turk Arch Pediatr. 2023 May;58(3):298-301. doi: 10.5152/TurkArchPediatr.2023.21363. PMID: 37144263; PMCID: PMC10210608. 4. Gondim-Silva KR, da-Silva JM, de Souza LAV, Guedes RCA. Neonatal pyridoxine administration long lastingly accelerates cortical spreading depression in male rats, without affecting anxiety-like behavior. Nutr Neurosci. 2021 May;24(5):363-370. doi: 10.1080/1028415X.2019.1632570. Epub 2019 Jun 21. PMID: 31221041.

In vitro protocol:

In vivo protocol:

1. Kendir-Demirkol Y, Jenny LA, Demirkol A, Özen M, Ayata A, Canatan D. The Protective Effects of Pyridoxine on Linezolid-Induced Hematological Toxicity, Hepatotoxicity, and Oxidative Stress in Rats. Turk Arch Pediatr. 2023 May;58(3):298-301. doi: 10.5152/TurkArchPediatr.2023.21363. PMID: 37144263; PMCID: PMC10210608. 2. Gondim-Silva KR, da-Silva JM, de Souza LAV, Guedes RCA. Neonatal pyridoxine administration long lastingly accelerates cortical spreading depression in male rats, without affecting anxiety-like behavior. Nutr Neurosci. 2021 May;24(5):363-370. doi: 10.1080/1028415X.2019.1632570. Epub 2019 Jun 21. PMID: 31221041.

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