Synonym
Ro 16-6491; Ro166491; Ro-16-6491.
IUPAC/Chemical Name
N-(2-Aminoethyl)-4-chlorobenzamide hydrochloride
InChi Key
ARUMZUNJBHSOQQ-UHFFFAOYSA-N
InChi Code
InChI=1S/C9H11ClN2O.ClH/c10-8-3-1-7(2-4-8)9(13)12-6-5-11;/h1-4H,5-6,11H2,(H,12,13);1H
SMILES Code
O=C(NCCN)C1=CC=C(Cl)C=C1.[H]Cl
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
235.11
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: DeJesus OT, Flores LG, Roberts AD, Dick DW, Bartlett RM, Murali D, Nickles RJ. Aromatic L-amino acid decarboxylase (AAAD) activity in rhesus macaque striatum after MAO-B inhibition by Ro 16-6491. Synapse. 2005 Apr;56(1):54-6. PubMed PMID: 15700282.
2: Mullan EL, Williams CH. Kinetics of inhibition of MAO-B by N-(2-aminoethyl)-p-chlorobenzamide (Ro 16-6491) and analogues. J Neural Transm Suppl. 1994;41:307-11. PubMed PMID: 7931243.
3: Annan N, Silverman RB. New analogues of N-(2-aminoethyl)-4-chlorobenzamide (Ro 16-6491). Some of the most potent monoamine oxidase-B inactivators. J Med Chem. 1993 Nov 26;36(24):3968-70. PubMed PMID: 8254625.
4: Cesura AM, Imhof R, Muggli-Maniglio D, Picotti GB, Da Prada M. Characterization of [3H]Ro 16-6491 binding to digitonin solubilized monoamine oxidase-B and purification of the enzyme from human platelets by affinity chromatography. Biochem Pharmacol. 1990 Jan 1;39(1):216-20. PubMed PMID: 2297358.
5: Cesura AM, Imhof R, Galva MD, Kettler R, Da Prada M. Interactions of the novel inhibitors of MAO-B Ro 19-6327 and Ro 16-6491 with the active site of the enzyme. Pharmacol Res Commun. 1988 Dec;20 Suppl 4:51-61. PubMed PMID: 3244727.
6: Cesura AM, Imhof R, Takacs B, Galva MD, Picotti GB, Da Prada M. [3H]Ro 16-6491, a selective probe for affinity labelling of monoamine oxidase type B in human brain and platelet membranes. J Neurochem. 1988 Apr;50(4):1037-43. PubMed PMID: 3126263.
7: Cesura AM, Galva MD, Imhof R, Da Prada M. Binding of [3H]Ro 16-6491, a reversible inhibitor of monoamine oxidase type B, to human brain mitochondria and platelet membranes. J Neurochem. 1987 Jan;48(1):170-6. PubMed PMID: 3794699.
8: Keller HH, Kettler R, Keller G, Da Prada M. Short-acting novel MAO inhibitors: in vitro evidence for the reversibility of MAO inhibition by moclobemide and Ro 16-6491. Naunyn Schmiedebergs Arch Pharmacol. 1987 Jan;335(1):12-20. PubMed PMID: 3574489.
9: Da Prada M, Kettler R, Keller HH, Bonetti EP, Imhof R. Ro 16-6491: a new reversible and highly selective MAO-B inhibitor protects mice from the dopaminergic neurotoxicity of MPTP. Adv Neurol. 1987;45:175-8. PubMed PMID: 2881442.
