MedKoo Cat#: 318202 | Name: Methocarbamol
Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Methocarbamol is a skeletal muscle relaxant. This compound is a carbamate of guaifenesin. Methocarbamol has been observed to bind to carbonic anhydrase I and II with 1000-fold more specificity for carbonic anhydrase I.

Chemical Structure

Methocarbamol
Methocarbamol
CAS#532-03-6

Theoretical Analysis

MedKoo Cat#: 318202

Name: Methocarbamol

CAS#: 532-03-6

Chemical Formula: C11H15NO5

Exact Mass: 241.0950

Molecular Weight: 241.24

Elemental Analysis: C, 54.77; H, 6.27; N, 5.81; O, 33.16

Price and Availability

Size Price Availability Quantity
10g USD 250.00 2 Weeks
25g USD 450.00 2 Weeks
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
Methocarbamol; Robaxin; Lumirelax; Metocarbamolo; Metocarbamol; Schwarz Brand of Methocarbamol; Shire Brand of Methocarbamol; Whitehall Robins Brand of Methocarbamol; Whitehall-Robins Brand of Methocarbamol;
IUPAC/Chemical Name
[2-hydroxy-3-(2-methoxyphenoxy)propyl] carbamate
InChi Key
GNXFOGHNGIVQEH-UHFFFAOYSA-N
InChi Code
InChI=1S/C11H15NO5/c1-15-9-4-2-3-5-10(9)16-6-8(13)7-17-11(12)14/h2-5,8,13H,6-7H2,1H3,(H2,12,14)
SMILES Code
COC1=CC=CC=C1OCC(COC(=O)N)O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Biological target:
Methocarbamol is an orally active central muscle relaxant and blocks muscular Nav1.4 channel.
In vitro activity:
Methocarbamol, at 2 mM, decreased the decay of endplate currents, slowed the decay of endplate potentials and reduced tetanic force of soleus muscles. The drug reversibly inhibited current flow through muscular Nav 1.4 channels, while neuronal Nav 1.7 channels were unaffected. Reference: Muscle Nerve. 2021 Jan;63(1):141-150. https://pubmed.ncbi.nlm.nih.gov/33043468/
In vivo activity:
TBD
Solvent mg/mL mM comments
Solubility
DMF 33.0 136.79
DMF:PBS (pH 7.2) (1:30) 0.0 0.12
DMSO 54.7 226.60
Ethanol 31.0 128.50
Water 3.0 12.44
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 241.24 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Zhang Y, Otto P, Qin L, Eiber N, Hashemolhosseini S, Kröger S, Brinkmeier H. Methocarbamol blocks muscular Nav 1.4 channels and decreases isometric force of mouse muscles. Muscle Nerve. 2021 Jan;63(1):141-150. doi: 10.1002/mus.27087. Epub 2020 Oct 27. PMID: 33043468. 2. Minai-Tehrani D, Toofani S, Yazdi F, Minai-Tehrani A, Mollasalehi H, Bakhtiari Ziabari K. Inhibition of pseudoperoxiadse activity of human red blood cell hemoglobin by methocarbamol. Int J Biol Macromol. 2017 Jan;94(Pt B):788-792. doi: 10.1016/j.ijbiomac.2016.09.049. Epub 2016 Sep 16. PMID: 27645932.
In vitro protocol:
1. Zhang Y, Otto P, Qin L, Eiber N, Hashemolhosseini S, Kröger S, Brinkmeier H. Methocarbamol blocks muscular Nav 1.4 channels and decreases isometric force of mouse muscles. Muscle Nerve. 2021 Jan;63(1):141-150. doi: 10.1002/mus.27087. Epub 2020 Oct 27. PMID: 33043468. 2. Minai-Tehrani D, Toofani S, Yazdi F, Minai-Tehrani A, Mollasalehi H, Bakhtiari Ziabari K. Inhibition of pseudoperoxiadse activity of human red blood cell hemoglobin by methocarbamol. Int J Biol Macromol. 2017 Jan;94(Pt B):788-792. doi: 10.1016/j.ijbiomac.2016.09.049. Epub 2016 Sep 16. PMID: 27645932.
In vivo protocol:
TBD
1: Dobek CE, Dipinto AA, Kuan AJ, Lam RW, Blumberger DM, Downar J, Daskalakis ZJ, Vila-Rodriguez F. Sinus Tachycardia Induced by Methocarbamol and Repetitive Transcranial Magnetic Stimulation (rTMS). Brain Stimul. 2016 Jan-Feb;9(1):156-8. doi: 10.1016/j.brs.2015.10.012. Epub 2015 Nov 24. PubMed PMID: 26792632. 2: Knych HK, Stanley SD, Seminoff KN, McKemie DS, Kass PH. Pharmacokinetics of methocarbamol and phenylbutazone in exercised Thoroughbred horses. J Vet Pharmacol Ther. 2016 Feb 29. doi: 10.1111/jvp.12298. [Epub ahead of print] PubMed PMID: 26924025. 3: Aljuhani O, Kopp BJ, Patanwala AE. Effect of Methocarbamol on Acute Pain After Traumatic Injury. Am J Ther. 2015 Oct 8. [Epub ahead of print] PubMed PMID: 26469684. 4: Emrich OM, Milachowski KA, Strohmeier M. [Methocarbamol in acute low back pain. A randomized double-blind controlled study]. MMW Fortschr Med. 2015 Jul;157 Suppl 5:9-16. doi: 10.1007/s15006-015-3307-x. Epub 2015 Jul 13. German. PubMed PMID: 26168743. 5: Essam HM, Abd-El Rahman MK. Smart manipulation of ratio spectra for resolving a pharmaceutical mixture of Methocarbamol and Paracetamol. Spectrochim Acta A Mol Biomol Spectrosc. 2015 Apr 15;141:1-9. doi: 10.1016/j.saa.2015.01.017. Epub 2015 Jan 21. PubMed PMID: 25659736. 6: Saraji M, Khayamian T, Hashemian Z. Extraction of methocarbamol from human plasma with a polypyrrole/multiwalled carbon nanotubes composite decorated with magnetic nanoparticles as an adsorbent followed by electrospray ionization ion mobility spectrometry detection. J Sep Sci. 2014 Dec;37(23):3518-25. doi: 10.1002/jssc.201400614. Epub 2014 Oct 21. PubMed PMID: 25243817. 7: Mansour AM, Shehab OR. Trapping of muscle relaxant methocarbamol degradation product by complexation with copper(II) ion: spectroscopic and quantum chemical studies. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jul 15;128:263-71. doi: 10.1016/j.saa.2014.02.142. Epub 2014 Mar 12. PubMed PMID: 24674917. 8: Helmy SA, El-Bedaiwy HM. Simultaneous determination of paracetamol and methocarbamol in human plasma By HPLC using UV detection with time programming: application to pharmacokinetic study. Drug Res (Stuttg). 2014 Jul;64(7):363-7. doi: 10.1055/s-0033-1358703. Epub 2013 Nov 20. PubMed PMID: 24258704. 9: Rumpler MJ, Colahan P, Sams RA. The pharmacokinetics of methocarbamol and guaifenesin after single intravenous and multiple-dose oral administration of methocarbamol in the horse. J Vet Pharmacol Ther. 2014 Feb;37(1):25-34. doi: 10.1111/jvp.12068. Epub 2013 Jul 17. PubMed PMID: 23859819. 10: Abdelwahab NS, Abdelrahman MM. Simultaneous determination of methocarbamol and Ibuprofen by first derivative synchronous fluorescence spectroscopic method in their binary mixture and spiked human plasma. J Fluoresc. 2014 Jan;24(1):129-35. doi: 10.1007/s10895-013-1276-9. Epub 2013 Aug 3. PubMed PMID: 23912962. 11: Draper WE, Bolfer L, Cottam E, McMichael M, Schubert T. Methocarbamol CRI for symptomatic treatment of pyrethroid intoxication: a report of three cases. J Am Anim Hosp Assoc. 2013 Sep-Oct;49(5):325-8. doi: 10.5326/JAAHA-MS-5835. Epub 2013 Mar 27. PubMed PMID: 23535756. 12: Pruitt BN 3rd. Methocarbamol suspension for the treatment of rhabdomyolysis in equines. Int J Pharm Compd. 2013 Sep-Oct;17(5):384-7. PubMed PMID: 24459784. 13: Kuo K, Odunayo A. Adjunctive therapy with intravenous lipid emulsion and methocarbamol for permethrin toxicity in 2 cats. J Vet Emerg Crit Care (San Antonio). 2013 Jul-Aug;23(4):436-41. doi: 10.1111/vec.12070. Epub 2013 Jul 15. PubMed PMID: 23855545. 14: El-Din MS, Eid M, Zeid AM. Simultaneous determination of methocarbamol and aspirin by RP-HPLC using fluorescence detection with time programming: its application to pharmaceutical dosage form. Luminescence. 2013 May-Jun;28(3):332-8. doi: 10.1002/bio.2386. Epub 2012 Jun 20. PubMed PMID: 22715138. 15: Looke TD, Kluth CT. Effect of preoperative intravenous methocarbamol and intravenous acetaminophen on opioid use after primary total hip and knee replacement. Orthopedics. 2013 Feb;36(2 Suppl):25-32. doi: 10.3928/01477447-20130122-54. PubMed PMID: 23379573. 16: Ali NW, Hegazy MA, Abdelkawy M, Abdelaleem EA. Simultaneous determination of methocarbamol and ibuprofen or diclofenac potassium using mean centering of the ratio spectra method. Acta Pharm. 2012 Jun;62(2):191-200. doi: 10.2478/v10007-012-0016-z. PubMed PMID: 22750817. 17: Minai-Tehrani D, Masoudnia A, Alavi S, Osmani R, Lotfi L, Asghari M, Pirsalehi M, Sobhani-Damavandifar Z. Interaction of methocarbamol and yeast sucrase induces enzyme inhibition. Drug Metabol Drug Interact. 2012;27(4):225-8. PubMed PMID: 23023692. 18: Gholivand MB, Khodadadian M. Rationally designed molecularly imprinted polymers for selective extraction of methocarbamol from human plasma. Talanta. 2011 Sep 15;85(3):1680-8. doi: 10.1016/j.talanta.2011.06.066. Epub 2011 Jul 2. PubMed PMID: 21807239. 19: Elkady EF. Simultaneous spectrophotometric determination of diclofenac potassium and methocarbamol in binary mixture using chemometric techniques and artificial neural networks. Drug Test Anal. 2011 Apr;3(4):228-33. doi: 10.1002/dta.216. Epub 2010 Dec 29. PubMed PMID: 21500367. 20: Walash M, Belal F, Eid M, EL Abass SA. Spectrofluorometric determination of methocarbamol in pharmaceutical preparations and human plasma. J Fluoresc. 2011 Mar;21(2):555-61. doi: 10.1007/s10895-010-0742-x. Epub 2010 Oct 9. PubMed PMID: 20936330.