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MedKoo Cat#: 522561 | Name: BRL37344 sodium salt
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

BRL37344 is a potent and selective β3-adrenergic receptor agonist. BRL37344 decreases nerve-evoked contractions in human detrusor smooth muscle isolated strips: role of BK channels. BRL37344 stimulates fuel oxidation by soleus muscle in vitro. BRL37344 increases glucose transport into L6 myocytes through a mechanism different from that of insulin.

Chemical Structure

BRL37344 sodium salt
BRL37344 sodium salt
CAS#127299-93-8 (sodium)

Theoretical Analysis

MedKoo Cat#: 522561

Name: BRL37344 sodium salt

CAS#: 127299-93-8 (sodium)

Chemical Formula: C19H21ClNNaO4

Exact Mass: 385.1057

Molecular Weight: 385.82

Elemental Analysis: C, 59.15; H, 5.49; Cl, 9.19; N, 3.63; Na, 5.96; O, 16.59

Price and Availability

Size Price Availability Quantity
10mg USD 350.00 2 Weeks
25mg USD 650.00 2 Weeks
50mg USD 950.00 2 Weeks
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Related CAS #
90730-96-4 (free acid) 114333-71-0 (free acid) 127299-93-8 (sodium)
Synonym
BRL37344 sodium; BRL-37344 sodium; BRL 37344 sodium,
IUPAC/Chemical Name
sodium 2-(4-((R)-2-(((R)-2-(3-chlorophenyl)-2-hydroxyethyl)amino)propyl)phenoxy)acetate
InChi Key
SNJIJYKMYQRHRC-WJKBNZMCSA-M
InChi Code
InChI=1S/C19H22ClNO4.Na/c1-13(21-11-18(22)15-3-2-4-16(20)10-15)9-14-5-7-17(8-6-14)25-12-19(23)24;/h2-8,10,13,18,21-22H,9,11-12H2,1H3,(H,23,24);/q;+1/p-1/t13-,18+;/m1./s1
SMILES Code
O=C(O[Na])COC1=CC=C(C[C@H](NC[C@@H](C2=CC=CC(Cl)=C2)O)C)C=C1
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
BRL 37344 sodium (BRL 37344A) is a specific β3-adrenergic receptor agonist.
In vitro activity:
BRL37344 (10 nM–100 μM) was very effective at inhibiting the amplitude, duration, and force of the 20 Hz EFS-induced contractions of human DSM isolated strips. BRL37344 (10 nM, 100 nM, 1 μM, 10 μM, and 100 μM) reduced the amplitude of the EFS-induced contractions by 11.6±3.9%, 22.9±8.2%, 33.3±10.3%, 45.5±10.4%, and 71.9±6.1%, respectively (n=4, N=3; Fig. 1A, C). Reference: Urology. 2013 Sep; 82(3): 744.e1–744.e7. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758792/
In vivo activity:
Application of BRL37344 for 4 weeks significantly decreased the serum ALT, AST, TGs, TC, LDL-C and FFA levels in the HFD-fed rats (P < 0.05). In contrast, the serum ALT, AST, TGs, TC, LDL-C and FFA levels among rats were markedly higher in the HFD + β3-ANT group (treated with L748337) than in the HFD group (P < 0.05). Reference: Mol Med. 2020 Jun 5;26(1):54. https://pubmed.ncbi.nlm.nih.gov/32503411/
Solvent mg/mL mM
Solubility
DMSO 5.0 12.96
PBS (pH 7.2) 10.0 25.92
Water 38.6 100.00
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 385.82 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Afeli SA, Rovner ES, Petkov GV. BRL37344, a β3-adrenergic receptor agonist, decreases nerve-evoked contractions in human detrusor smooth muscle isolated strips: role of BK channels. Urology. 2013 Sep;82(3):744.e1-7. doi: 10.1016/j.urology.2013.05.027. Epub 2013 Jul 26. PMID: 23890664; PMCID: PMC3758792. 2. Wang Z, Li S, Wang R, Guo L, Xu D, Zhang T, Xu Y, Wang W, Wang M, Gan Z, Wang X. The protective effects of the β3 adrenergic receptor agonist BRL37344 against liver steatosis and inflammation in a rat model of high-fat diet-induced nonalcoholic fatty liver disease (NAFLD). Mol Med. 2020 Jun 5;26(1):54. doi: 10.1186/s10020-020-00164-4. PMID: 32503411; PMCID: PMC7275314. 3. Wang X, Wang R, Liu G, Dong J, Zhao G, Tian J, Sun J, Jia X, Wei L, Wang Y, Li W. The β3 Adrenergic Receptor Agonist BRL37344 Exacerbates Atrial Structural Remodeling Through iNOS Uncoupling in Canine Models of Atrial Fibrillation. Cell Physiol Biochem. 2016;38(2):514-30. doi: 10.1159/000438647. Epub 2016 Feb 1. PMID: 26828873.
In vitro protocol:
1. Afeli SA, Rovner ES, Petkov GV. BRL37344, a β3-adrenergic receptor agonist, decreases nerve-evoked contractions in human detrusor smooth muscle isolated strips: role of BK channels. Urology. 2013 Sep;82(3):744.e1-7. doi: 10.1016/j.urology.2013.05.027. Epub 2013 Jul 26. PMID: 23890664; PMCID: PMC3758792.
In vivo protocol:
1. Wang Z, Li S, Wang R, Guo L, Xu D, Zhang T, Xu Y, Wang W, Wang M, Gan Z, Wang X. The protective effects of the β3 adrenergic receptor agonist BRL37344 against liver steatosis and inflammation in a rat model of high-fat diet-induced nonalcoholic fatty liver disease (NAFLD). Mol Med. 2020 Jun 5;26(1):54. doi: 10.1186/s10020-020-00164-4. PMID: 32503411; PMCID: PMC7275314. 2. Wang X, Wang R, Liu G, Dong J, Zhao G, Tian J, Sun J, Jia X, Wei L, Wang Y, Li W. The β3 Adrenergic Receptor Agonist BRL37344 Exacerbates Atrial Structural Remodeling Through iNOS Uncoupling in Canine Models of Atrial Fibrillation. Cell Physiol Biochem. 2016;38(2):514-30. doi: 10.1159/000438647. Epub 2016 Feb 1. PMID: 26828873.
1: Dobrek Ł, Baranowska A, Thor PJ. Heart rate variability after BRL37344, a beta-3 agonist, in experimental bladder outlet obstruction. Postepy Hig Med Dosw (Online). 2013 Aug 12;67:848-58. PubMed PMID: 24018450. 2: Afeli SA, Rovner ES, Petkov GV. BRL37344, a β3-adrenergic receptor agonist, decreases nerve-evoked contractions in human detrusor smooth muscle isolated strips: role of BK channels. Urology. 2013 Sep;82(3):744.e1-7. doi: 10.1016/j.urology.2013.05.027. Epub 2013 Jul 26. PubMed PMID: 23890664; PubMed Central PMCID: PMC3758792. 3: Ngala RA, O'Dowd J, Wang SJ, Stocker C, Cawthorne MA, Arch JR. Beta2-adrenoceptors and non-beta-adrenoceptors mediate effects of BRL37344 and clenbuterol on glucose uptake in soleus muscle: studies using knockout mice. Br J Pharmacol. 2009 Dec;158(7):1676-82. doi: 10.1111/j.1476-5381.2009.00472.x. Epub . PubMed PMID: 19912225; PubMed Central PMCID: PMC2801208. 4: Ngala RA, O'Dowd J, Wang SJ, Agarwal A, Stocker C, Cawthorne MA, Arch JR. Metabolic responses to BRL37344 and clenbuterol in soleus muscle and C2C12 cells via different atypical pharmacologies and beta2-adrenoceptor mechanisms. Br J Pharmacol. 2008 Oct;155(3):395-406. doi: 10.1038/bjp.2008.244. Epub 2008 Jun 16. PubMed PMID: 18552870; PubMed Central PMCID: PMC2567876. 5: Kubota Y, Nakahara T, Yunoki M, Mitani A, Maruko T, Sakamoto K, Ishii K. Inhibitory mechanism of BRL37344 on muscarinic receptor-mediated contractions of the rat urinary bladder smooth muscle. Naunyn Schmiedebergs Arch Pharmacol. 2002 Sep;366(3):198-203. Epub 2002 Jul 5. PubMed PMID: 12172701. 6: Board M, Doyle P, Cawthorne MA. BRL37344, but not CGP12177, stimulates fuel oxidation by soleus muscle in vitro. Eur J Pharmacol. 2000 Oct 6;406(1):33-40. PubMed PMID: 11011029. 7: Horinouchi T, Koshikawa H, Koike K. Effect of bupranolol for BRL37344 and noradrenaline-induced relaxations mediating atypical beta/beta3-adrenoceptor in rat oesophageal muscularis mucosae. Gen Pharmacol. 1999 Aug;33(2):173-8. PubMed PMID: 10461855. 8: Tanishita T, Shimizu Y, Minokoshi Y, Shimazu T. The beta3-adrenergic agonist BRL37344 increases glucose transport into L6 myocytes through a mechanism different from that of insulin. J Biochem. 1997 Jul;122(1):90-5. PubMed PMID: 9276675. 9: Ida K, Hashimoto K, Kamiya M, Muto S, Nakamura Y, Kato K, Mizota M. Stereoselective action of (R*,R*)-(+/-)-methyl-4-[2-[2-hydroxy-2-(3-chlorophenyl)ethylamino] propyl]-phenoxyacetic acid (BRL37344) on beta-adrenoceptors and metabolic chiral inversion. Biochem Pharmacol. 1996 Nov 22;52(10):1521-7. PubMed PMID: 8937466. 10: Chang CJ, Kao JT, Lee TL, Lai CW, Cheng JT. Comparison of isoproterenol with BRL37344 in activation of beta 3-adrenoceptors to inhibit the uptake of [14C]deoxy-D-glucose and translocation of glucose transporter (GLUT4) to membrane fraction in rat adipocytes. J Auton Nerv Syst. 1996 Nov 6;61(2):191-4. PubMed PMID: 8946341. 11: Yamakawa A, Tanaka E, Nakano S. Effect of the adrenergic beta 3-agonist, BRL37344, on heat production by brown adipocytes in obese and in older rats. Tokai J Exp Clin Med. 1994 Dec;19(3-6):139-42. PubMed PMID: 7570685.