Synonym
Resorufin ethyl ether; 7-Ethoxyresorufin
IUPAC/Chemical Name
7-ethoxy-3H-phenoxazin-3-one
InChi Key
CRCWUBLTFGOMDD-UHFFFAOYSA-N
InChi Code
InChI=1S/C14H11NO3/c1-2-17-10-4-6-12-14(8-10)18-13-7-9(16)3-5-11(13)15-12/h3-8H,2H2,1H3
SMILES Code
O=C1C=CC2=NC3=C(C=C(OCC)C=C3)OC2=C1
Appearance
To be determined
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 -4 C for short term (days to weeks) or -20 C for long term(months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
241.25
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Lewis BC, Korprasertthaworn P, Miners JO. Impaired dacarbazine activation and
7-ethoxyresorufin deethylation in vitro by polymorphic variants of CYP1A1 and
CYP1A2: implications for cancer therapy. Pharmacogenet Genomics. 2016
Oct;26(10):453-61. doi: 10.1097/FPC.0000000000000236. PMID: 27428168.
2: Pacifici GM, Park SS, Gelboin HV, Rane A. 7-Ethoxycoumarin and
7-ethoxyresorufin O-deethylase in human foetal and adult liver: studies with
monoclonal antibodies. Pharmacol Toxicol. 1988 Jul;63(1):26-9. doi:
10.1111/j.1600-0773.1988.tb00903.x. PMID: 3261011.
3: Heinrich P, Petschick LL, Northcott GL, Tremblay LA, Ataria JM, Braunbeck T.
Assessment of cytotoxicity, genotoxicity and 7-ethoxyresorufin-O-deethylase
(EROD) induction in sediment extracts from New Zealand urban estuaries.
Ecotoxicology. 2017 Mar;26(2):211-226. doi: 10.1007/s10646-016-1756-1. Epub 2017
Jan 12. PMID: 28083773.
4: Fenet H, Gomez E, Rosain D, Casellas C. Polycyclic aromatic hydrocarbon
metabolites and 7-ethoxyresorufin O-deethylase activity in caged European eels.
Arch Environ Contam Toxicol. 2006 Aug;51(2):232-6. doi:
10.1007/s00244-005-0064-1. Epub 2006 Jun 1. PMID: 16783620.
5: Zhu S, Wu J, Liu S, Jiang T, Deng Y. Phe-125 and Phe-226 of pig cytochrome
P450 1A2 stabilize the binding of aflatoxin B1 and 7-ethoxyresorufin
through the key CH/π interactions. Biochem Pharmacol. 2019 Aug;166:292-299. doi:
10.1016/j.bcp.2019.05.031. Epub 2019 Jun 4. PMID: 31173723.
6: Wagner L, Zlabek V, Trattner S, Zamaratskaia G. In vitro inhibition of
7-ethoxyresorufin-O-deethylase (EROD) and p-nitrophenol hydroxylase (PNPH)
activities by sesamin in hepatic microsomes from two fish species. Mol Biol Rep.
2013 Jan;40(1):457-62. doi: 10.1007/s11033-012-2080-3. Epub 2012 Oct 12. PMID:
23065221.
7: Pacifici GM, Park SS, Gelboin HW, Rane A. Ontogenic development of
7-ethoxycoumarin and 7-ethoxyresorufin O-deethylase in the rat and effect of
monoclonal antibodies. Pharmacol Toxicol. 1988 Feb;62(2):101-3. doi:
10.1111/j.1600-0773.1988.tb01854.x. PMID: 3258422.
8: Heinrich-Hirsch B, Hofmann D, Webb J, Neubert D. Activity of aldrinepoxidase,
7-ethoxycoumarin-O-deethylase and 7-ethoxyresorufin-O-deethylase during the
development of chick embryos in ovo. Arch Toxicol. 1990;64(2):128-34. doi:
10.1007/BF01974398. PMID: 2350231.
9: Chang TK, Waxman DJ. Enzymatic analysis of cDNA-expressed human CYP1A1,
CYP1A2, and CYP1B1 with 7-ethoxyresorufin as substrate. Methods Mol Biol.
2006;320:85-90. doi: 10.1385/1-59259-998-2:85. PMID: 16719376.
10: Carlsson C, Pärt P. 7-Ethoxyresorufin O-deethylase induction in rainbow
trout gill epithelium cultured on permeable supports: asymmetrical distribution
of substrate metabolites. Aquat Toxicol. 2001 Sep;54(1-2):29-38. doi:
10.1016/s0166-445x(00)00184-3. PMID: 11451423.
