Ninhydrin | CAS#485-47-2 | Derivatizing reagent

MedKoo Cat#: 145029 | Name: Ninhydrin

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Ninhydrin is used for the detection of latent fingermarks on porous surfaces. Ninhydrin, a widely used derivatizing reagent, is widely used as a reagent for the detection of free amino and carboxyl groups in proteins and peptides. It is an irritant to mammalian skin. A ninhydrin test allows for amino acids to be identified when combined with ninhydrin by turning a dark purple color, Ruhemann's purple.

Chemical Structure

Ninhydrin

CAS#485-47-2

Theoretical Analysis

MedKoo Cat#: 145029

Name: Ninhydrin

CAS#: 485-47-2

Chemical Formula: C9H6O4

Exact Mass: 178.0266

Molecular Weight: 178.14

Elemental Analysis: C, 60.68; H, 3.40; O, 35.92

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.

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Related CAS #

No Data

Synonym

NINHYDRIN; INDAN-1,2,3-TRIONE; TRIKETOHYDRINDEN HYDRATE; 2,2-DIHYDROXY-1,3-INDANDIONE;

IUPAC/Chemical Name

2,2-dihydroxy-1H-indene-1,3(2H)-dione

InChi Key

FEMOMIGRRWSMCU-UHFFFAOYSA-N

InChi Code

1S/C9H6O4/c10-7-5-3-1-2-4-6(5)8(11)9(7,12)13/h1-4,12-13H

SMILES Code

OC1(O)C(=O)C2=C(C=CC=C2)C1=O

Appearance

To be determined

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 -4 C for short term (days to weeks) or -20 C for long term(months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Instruction

View handling instruction

Preparing Stock Solutions

The following data is based on the product molecular weight 178.14 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

1: Friedman M. Applications of the ninhydrin reaction for analysis of amino acids, peptides, and proteins to agricultural and biomedical sciences. J Agric Food Chem. 2004 Feb 11;52(3):385-406. doi: 10.1021/jf030490p. PMID: 14759124. 2: Hansen DB, Joullie MM. The development of novel ninhydrin analogues. Chem Soc Rev. 2005 May;34(5):408-17. doi: 10.1039/b315496n. Epub 2005 Feb 11. PMID: 15852153. 3: Chen Y, Tang J, Wang S, Zhang L. Ninhydrin-functionalized chitosan for selective removal of Pb(II) ions: Characterization and adsorption performance. Int J Biol Macromol. 2021 Apr 30;177:29-39. doi: 10.1016/j.ijbiomac.2021.02.110. Epub 2021 Feb 17. PMID: 33607139. 4: Jong JAW, Guo Y, Hazenbrink D, Douka S, Verdijk D, van der Zwan J, Houben K, Baldus M, Scheiner KC, Dalebout R, Verhaar MC, Smakman R, Hennink WE, Gerritsen KGF, van Nostrum CF. A Ninhydrin-Type Urea Sorbent for the Development of a Wearable Artificial Kidney. Macromol Biosci. 2020 Mar;20(3):e1900396. doi: 10.1002/mabi.201900396. Epub 2020 Feb 17. PMID: 32065727. 5: Haeger G, Bongaerts J, Siegert P. A convenient ninhydrin assay in 96-well format for amino acid-releasing enzymes using an air-stable reagent. Anal Biochem. 2022 Oct 1;654:114819. doi: 10.1016/j.ab.2022.114819. Epub 2022 Jul 15. PMID: 35839914. 6: Guedes PHG, Brussasco JG, Moço ACR, Moraes DD, Flauzino JMR, Luz LFG, Almeida MTG, Soares MMCN, Oliveira RJ, Madurro JM, Brito-Madurro AG. Ninhydrin as a novel DNA hybridization indicator applied to a highly reusable electrochemical genosensor for Candida auris. Talanta. 2021 Dec 1;235:122694. doi: 10.1016/j.talanta.2021.122694. Epub 2021 Jul 8. PMID: 34517578. 7: Anantharaman S, Padmarajaiah N, Al-Tayar NGS, Shrestha AK. Ninhydrin-sodium molybdate chromogenic analytical probe for the assay of amino acids and proteins. Spectrochim Acta A Mol Biomol Spectrosc. 2017 Feb 15;173:897-903. doi: 10.1016/j.saa.2016.10.040. Epub 2016 Oct 21. PMID: 27821372. 8: Cowan TM. Commentary. Clin Chem. 2011 Apr;57(4):548-9. doi: 10.1373/clinchem.2010.160333. PMID: 21444739. 9: Carter DO, Yellowlees D, Tibbett M. Using ninhydrin to detect gravesoil. J Forensic Sci. 2008 Mar;53(2):397-400. doi: 10.1111/j.1556-4029.2008.00681.x. PMID: 18366573. 10: Ali MFB, Salman BI, Hussein SA, Marzouq MA. Applicability of ninhydrin as a fluorescent reagent for estimation of teicoplanin in human plasma using salting- out assisted liquid-liquid extraction technique. Luminescence. 2020 Nov;35(7):1118-1124. doi: 10.1002/bio.3824. Epub 2020 May 21. PMID: 32436350. 11: Yavari I, Khajeh-Khezri A. A synthesis of oxo-thioxo[3.3.3]propellanes from dithiocarbamates and ninhydrin-malononitrile adduct. Mol Divers. 2017 Nov;21(4):849-854. doi: 10.1007/s11030-017-9761-8. Epub 2017 Jun 26. PMID: 28653127. 12: DHUNER KG, EDSHAGE S, WILHELM A. Ninhydrin test--an objective method for testing local anesthetic drugs. Acta Anaesthesiol Scand. 1960;4:189-98. doi: 10.1111/j.1399-6576.1960.tb00074.x. PMID: 13722434. 13: Zatorski JM, Montalbine AN, Ortiz-Cárdenas JE, Pompano RR. Quantification of fractional and absolute functionalization of gelatin hydrogels by optimized ninhydrin assay and 1H NMR. Anal Bioanal Chem. 2020 Sep;412(24):6211-6220. doi: 10.1007/s00216-020-02792-5. Epub 2020 Jul 3. PMID: 32617761; PMCID: PMC7484248. 14: Omar MA, Nagy DM, Halim ME. Utility of ninhydrin reagent for spectrofluorimetric determination of heptaminol in human plasma. Luminescence. 2018 Sep;33(6):1107-1112. doi: 10.1002/bio.3516. Epub 2018 Jul 3. PMID: 29968975. 15: Lu Z, Jia Y, Chen X, Li P. Organocatalytic Regio- and Enantioselective [3 + 2]-Annulations of Ninhydrin-Derived Morita-Baylis-Hillman Carbonates with 3-Methyleneoxindoles. J Org Chem. 2022 Mar 4;87(5):3184-3194. doi: 10.1021/acs.joc.1c02917. Epub 2022 Feb 8. PMID: 35133821. 16: Wang KK, Li YL, Jing J, Chen R, Zhao NN, Li ZH, Wang MY, Ji SK. Synthesis of 1,3,5-trisubstituted pyrazoles via 1,3-dipolar cycloaddition of nitrile imines with ninhydrin-derived Morita-Baylis-Hillman carbonates. Org Biomol Chem. 2022 Aug 31;20(34):6923-6930. doi: 10.1039/d2ob01253g. PMID: 35979893. 17: Almog J, Glasner H. Ninhydrin thiohemiketals: basic research towards improved fingermark detection techniques employing nano-technology. J Forensic Sci. 2010 Jan;55(1):215-20. doi: 10.1111/j.1556-4029.2009.01231.x. Epub 2009 Dec 2. PMID: 20002273. 18: Shukla Y, Antony M, Gupta KP, Mehrotra NK. Tumour-promoting activity of ninhydrin on mouse skin. Food Chem Toxicol. 1994 Jul;32(7):651-4. doi: 10.1016/0278-6915(94)90009-4. PMID: 8045478. 19: Rousseau M, Ledroit P, Malo M, Henrot D, Guille H. Fingermarks development on gloves: Relative efficiency of 1,2 Indanedione/ZnCl2, ninhydrin and wet powder. Sci Justice. 2020 Sep;60(5):473-479. doi: 10.1016/j.scijus.2020.07.002. Epub 2020 Jul 10. PMID: 32873387. 20: Lee MR, Kim CS, Park T, Choi YS, Lee KH. Optimization of the ninhydrin reaction and development of a multiwell plate-based high-throughput proline detection assay. Anal Biochem. 2018 Sep 1;556:57-62. doi: 10.1016/j.ab.2018.06.022. Epub 2018 Jun 23. PMID: 29944872.