FALGPA (N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala) is a synthetic colorimetric substrate widely used to measure the activity of collagenase and other matrix metalloproteinases (MMPs). It works by mimicking the collagen triple helix cleavage site, and its hydrolysis by collagenase releases a chromophore that can be detected spectrophotometrically, typically at 345 nm. The cleavage rate is proportional to enzyme activity, with reported Km values for Clostridium histolyticum collagenase around 0.2–1.0 mM, depending on assay conditions. FALGPA is not a natural substrate, so its turnover number (kcat) is relatively low compared to native collagen. It remains a convenient tool for screening collagenase inhibitors or quantifying enzyme activity in biochemical assays due to its simple, direct readout and reasonable specificity for MMP-1 and MMP-8 compared to other proteases
MedKoo Cat#: 126030
Name: FALGPA TFA
CAS#: N/A
Chemical Formula: C25H33F3N4O9
Exact Mass: 590.2200
Molecular Weight: 590.55
Elemental Analysis: C, 50.85; H, 5.63; F, 9.65; N, 9.49; O, 24.38
The following data is based on the product molecular weight 590.55 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
