Also named as Difluorocarboxyfluorescein NHS Ester, 6-isomer. It (also known as Oregon Green™ 488 Carboxylic Acid, Succinimidyl Ester, 6-isomer) is an amine-reactive fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range.
MedKoo Cat#: 122779
Name: AUN51827
CAS#: 2702251-82-7
Chemical Formula: C25H13F2NO9
Exact Mass: 509.0600
Molecular Weight: 509.37
Elemental Analysis: C, 58.95; H, 2.57; F, 7.46; N, 2.75; O, 28.27
The following data is based on the product molecular weight 509.37 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
