N4-Acetylcytidine | CAS#3768-18-1 | BV-2 microglia activator

MedKoo Cat#: 112006 | Name: N4-Acetylcytidine

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

N4-Acetylcytidine is a catabolite of cytidine. It activates BV-2 microglia when used at a concentration of 0.3 mM, an effect that can be blocked by the adenosine A2A receptor antagonist SCH 58261. It also increases protein levels of the NOD-like receptor protein 3 (NLRP3) inflammasome, an effect that can be blocked by high mobility group box 1 (HMGB1) siRNA in BV-2 microglia. Urine levels of N4-acetylcytidine are increased in mice with tumors induced by 3-methylcholanthrene. N4-Acetylcytidine is also found as a post-transcriptional modification in RNA.

Chemical Structure

N4-Acetylcytidine

CAS#3768-18-1

Theoretical Analysis

MedKoo Cat#: 112006

Name: N4-Acetylcytidine

CAS#: 3768-18-1

Chemical Formula: C11H15N3O6

Exact Mass: 285.0961

Molecular Weight: 285.26

Elemental Analysis: C, 46.32; H, 5.30; N, 14.73; O, 33.65

Price and Availability

Size	Price	Availability
1g	USD 240.00	2 Weeks
5g	USD 500.00	2 Weeks
10g	USD 740.00	2 Weeks
25g	USD 1,110.00	2 Weeks

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Related CAS #

No Data

Synonym

ac4C; N4-Acetylcytidine; N4Acetylcytidine; N4 Acetylcytidine

IUPAC/Chemical Name

N-(1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-2-oxo-1,2-dihydropyrimidin-4-yl)acetamide

InChi Key

NIDVTARKFBZMOT-PEBGCTIMSA-N

InChi Code

InChI=1S/C11H15N3O6/c1-5(16)12-7-2-3-14(11(19)13-7)10-9(18)8(17)6(4-15)20-10/h2-3,6,8-10,15,17-18H,4H2,1H3,(H,12,13,16,19)/t6-,8-,9-,10-/m1/s1

SMILES Code

O[C@H]1[C@@]([H])(O[C@@H]([C@H]1O)CO)N2C(N=C(NC(C)=O)C=C2)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Instruction

View handling instruction

	Solvent	mg/mL	mM
Solubility
	DMSO	1.0	3.51
	PBS (pH:7.2)	2.0	7.01

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 285.26 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

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Uncovering N4-Acetylcytidine-Related mRNA Modification Pattern and Landscape of Stemness and Immunity in Hepatocellular Carcinoma. Front Cell Dev Biol. 2022 Apr 14;10:861000. doi: 10.3389/fcell.2022.861000. PMID: 35493106; PMCID: PMC9046676. 5: Geng Z, Liu P, Yuan L, Zhang K, Lin J, Nie X, Jiang H, Li B, Liu T, Zhang B. Electroacupuncture attenuates ac4C modification of P16 mRNA in the ovarian granulosa cells of a mouse model premature ovarian failure. Acupunct Med. 2022 Apr 27:9645284221085284. doi: 10.1177/09645284221085284. Epub ahead of print. PMID: 35475376. 6: Broly M, Polevoda BV, Awayda KM, Tong N, Lentini J, Besnard T, Deb W, O'Rourke D, Baptista J, Ellard S, Almannai M, Hashem M, Abdulwahab F, Shamseldin H, Al-Tala S, Alkuraya FS, Leon A, van Loon RLE, Ferlini A, Sanchini M, Bigoni S, Ciorba A, van Bokhoven H, Iqbal Z, Al-Maawali A, Al-Murshedi F, Ganesh A, Al- Mamari W, Lim SC, Pais LS, Brown N, Riazuddin S, Bézieau S, Fu D, Isidor B, Cogné B, O'Connell MR. THUMPD1 bi-allelic variants cause loss of tRNA acetylation and a syndromic neurodevelopmental disorder. Am J Hum Genet. 2022 Apr 7;109(4):587-600. doi: 10.1016/j.ajhg.2022.02.001. Epub 2022 Feb 22. PMID: 35196516; PMCID: PMC9069073. 7: Li N, Rana TM. Regulation of antiviral innate immunity by chemical modification of viral RNA. Wiley Interdiscip Rev RNA. 2022 Feb 12:e1720. doi: 10.1002/wrna.1720. Epub ahead of print. PMID: 35150188. 8: Wang K, Zhou LY, Liu F, Lin L, Ju J, Tian PC, Liu CY, Li XM, Chen XZ, Wang T, Wang F, Wang SC, Zhang J, Zhang YH, Tian JW, Wang K. PIWI-Interacting RNA HAAPIR Regulates Cardiomyocyte Death After Myocardial Infarction by Promoting NAT10-Mediated ac4 C Acetylation of Tfec mRNA. Adv Sci (Weinh). 2022 Mar;9(8):e2106058. doi: 10.1002/advs.202106058. Epub 2022 Feb 9. PMID: 35138696; PMCID: PMC8922123. 9: Zhang G, Luo W, Lyu J, Yu ZG, Huang G. CNNLSTMac4CPred: A Hybrid Model for N4-Acetylcytidine Prediction. 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