NBD-Pen | CAS#1955505-54-0 | fluorescence probe

MedKoo Cat#: 561365 | Name: NBD-Pen

Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

NBD-Pen high-sensitivity, specific fluorescence probe for lipid radicals. NBD-Pen directly detected lipid radicals in living cells by turn-on fluorescence. In a rat model of hepatic carcinoma induced by diethylnitrosamine (DEN), NBD-Pen detected lipid radical generation within 1 hour of DEN administration.

Chemical Structure

NBD-Pen

CAS#1955505-54-0

Theoretical Analysis

MedKoo Cat#: 561365

Name: NBD-Pen

CAS#: 1955505-54-0

Chemical Formula: C19H30N5O4

Exact Mass: 392.2298

Molecular Weight: 392.48

Elemental Analysis: C, 58.15; H, 7.70; N, 17.84; O, 16.31

Price and Availability

Size	Price	Availability	Quantity
10mg	USD 450.00
50mg	USD 1,650.00
100mg	USD 2,650.00

Bulk Inquiry

Buy Now

Add to Cart

Related CAS #

No Data

Synonym

NBD-Pen; NBD Pen; NBDPen;

IUPAC/Chemical Name

2,2,6-Trimethyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)-6-pentylpiperidine-1-oxyl

InChi Key

NLURORPZLBRXMF-UHFFFAOYSA-N

InChi Code

InChI=1S/C19H30N5O4/c1-5-6-7-10-19(4)12-14(11-18(2,3)24(19)27)21-17-9-8-15(23(25)26)16-13-20-28-22(16)17/h8-9,13-14,20-21H,5-7,10-12H2,1-4H3

SMILES Code

[O]N1C(C)(C)CC(NC2=CC=C([N+]([O-])=O)C3=CNON32)CC1(C)CCCCC

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

NBD-Pen high-sensitivity, specific fluorescence probe for lipid radicals.

In vitro activity:

To evaluate radical-scavenging activity, this study used electron paramagnetic resonance-spin trapping methods and a fluorescence probe for lipid radicals, NBD-Pen, that this study had developed. Reference: J Am Soc Nephrol. 2020 Feb;31(2):280-296. https://pubmed.ncbi.nlm.nih.gov/31767624/

In vivo activity:

This study describes the first high-sensitivity, specific fluorescence probe for lipid radicals, 2,2,6-trimethyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)-6-pentylpiperidine-1-oxyl (NBD-Pen). In a rat model of hepatic carcinoma induced by diethylnitrosamine (DEN), NBD-Pen detected lipid radical generation within 1 h of DEN administration. Reference: Nat Chem Biol. 2016 Aug;12(8):608-13. https://pubmed.ncbi.nlm.nih.gov/27294322/

Preparing Stock Solutions

The following data is based on the product molecular weight 392.48 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Mishima E, Sato E, Ito J, Yamada KI, Suzuki C, Oikawa Y, Matsuhashi T, Kikuchi K, Toyohara T, Suzuki T, Ito S, Nakagawa K, Abe T. Drugs Repurposed as Antiferroptosis Agents Suppress Organ Damage, Including AKI, by Functioning as Lipid Peroxyl Radical Scavengers. J Am Soc Nephrol. 2020 Feb;31(2):280-296. doi: 10.1681/ASN.2019060570. Epub 2019 Nov 25. PMID: 31767624; PMCID: PMC7003311. 2. Ishida Y, Okamoto Y, Matsuoka Y, Tada A, Janprasit J, Yamato M, Morales NP, Yamada KI. Detection and inhibition of lipid-derived radicals in low-density lipoprotein. Free Radic Biol Med. 2017 Dec;113:487-493. doi: 10.1016/j.freeradbiomed.2017.10.388. Epub 2017 Oct 28. PMID: 29107744. 3. Yamada K, Mito F, Matsuoka Y, Ide S, Shikimachi K, Fujiki A, Kusakabe D, Ishida Y, Enoki M, Tada A, Ariyoshi M, Yamasaki T, Yamato M. Fluorescence probes to detect lipid-derived radicals. Nat Chem Biol. 2016 Aug;12(8):608-13. doi: 10.1038/nchembio.2105. Epub 2016 Jun 13. PMID: 27294322.

In vitro protocol:

In vivo protocol:

1. Ishida Y, Okamoto Y, Matsuoka Y, Tada A, Janprasit J, Yamato M, Morales NP, Yamada KI. Detection and inhibition of lipid-derived radicals in low-density lipoprotein. Free Radic Biol Med. 2017 Dec;113:487-493. doi: 10.1016/j.freeradbiomed.2017.10.388. Epub 2017 Oct 28. PMID: 29107744. 2. Yamada K, Mito F, Matsuoka Y, Ide S, Shikimachi K, Fujiki A, Kusakabe D, Ishida Y, Enoki M, Tada A, Ariyoshi M, Yamasaki T, Yamato M. Fluorescence probes to detect lipid-derived radicals. Nat Chem Biol. 2016 Aug;12(8):608-13. doi: 10.1038/nchembio.2105. Epub 2016 Jun 13. PMID: 27294322.

1: Yamada K, Mito F, Matsuoka Y, Ide S, Shikimachi K, Fujiki A, Kusakabe D, Ishida Y, Enoki M, Tada A, Ariyoshi M, Yamasaki T, Yamato M. Fluorescence probes to detect lipid-derived radicals. Nat Chem Biol. 2016 Aug;12(8):608-13. doi: 10.1038/nchembio.2105. Epub 2016 Jun 13. PubMed PMID: 27294322.