Ac4ManNDAz is a cell-permeable photo-crosslinking probe for studying glycoprotein or glycolipid interactions. The compound is an unnatural diazirine-containing monosaccharide building block, which acts as a photo-crosslinker. Once inside the cell, Ac4ManNDAz is metabolized and incorporated as photo-crosslinking sialic acid analogs into cell surface glycoproteins. Upon photoactivation with UV irradiation, the sialic acid appended diazirine is activated to a reactive carbene that covalently crosslinks to nearby molecules.
MedKoo Cat#: 466247
Name: Ac4ManNDAz
CAS#: 1015698-39-1
Chemical Formula: C19H27N3O10
Exact Mass: 457.1696
Molecular Weight: 457.44
Elemental Analysis: C, 49.89; H, 5.95; N, 9.19; O, 34.98
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| Ethanol | 45.7 | 100.00 | |
| DMSO | 45.7 | 100.00 |
The following data is based on the product molecular weight 457.44 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
