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MedKoo Cat#: 406197 | Name: SL327
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

SL327 is a MEK inhibitor. SL327 blocks acquisition but not expression of lithium-induced conditioned place aversion: a behavioral and immunohistochemical study. SL327 had no affect on acquisition, expression, or extinction of EtOH-induced CPP in mice despite causing significant reduction of pERK levels in multiple brain regions. Additionally, although SL327 caused a generalized depression of locomotor activity, it did not prevent the development of EtOH-sensitization.

Chemical Structure

SL327
SL327
CAS#305350-87-2

Theoretical Analysis

MedKoo Cat#: 406197

Name: SL327

CAS#: 305350-87-2

Chemical Formula: C16H12F3N3S

Exact Mass: 335.0704

Molecular Weight: 335.35

Elemental Analysis: C, 57.31; H, 3.61; F, 17.00; N, 12.53; S, 9.56

Price and Availability

Size Price Availability Quantity
50mg USD 450.00 2 Weeks
100mg USD 750.00 2 Weeks
200mg USD 1,250.00 2 Weeks
500mg USD 2,650.00 2 Weeks
1g USD 4,250.00 2 Weeks
2g USD 6,450.00 2 Weeks
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Related CAS #
No Data
Synonym
SL327; SL 327; SL-327.
IUPAC/Chemical Name
(E)-3-amino-3-((4-aminophenyl)thio)-2-(2-(trifluoromethyl)phenyl)acrylonitrile
InChi Key
JLOXTZFYJNCPIS-SQFISAMPSA-N
InChi Code
InChI=1S/C16H12F3N3S/c17-16(18,19)14-4-2-1-3-12(14)13(9-20)15(22)23-11-7-5-10(21)6-8-11/h1-8H,21-22H2/b15-13-
SMILES Code
N#C/C(C1=CC=CC=C1C(F)(F)F)=C(N)/SC2=CC=C(N)C=C2
Appearance
white solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
        
Product Data
Product Data
Instruction
View handling instruction
Biological target:
SL327 inhibits ERK1, MKK/p38, MKK4, JNK, and PKC at high concentrations (IC50s = >50, 21, >100, >100, >10 μM, respectively).
In vitro activity:
After experimental CNS hemorrhage, the breakdown of hemoglobin can cause iron-dependent neuronal damage, which heme oxygenase (HO) inhibitors may alleviate. U0126, SL327, and FR180204 reduced baseline HO activity by 35-50%, protecting against widespread neuronal injury caused by hemoglobin exposure. The results suggest that the protective effect of MEK and ERK inhibitors may be linked to a reduction in HO activity against heme-mediated neuronal damage. Reference: Neuropharmacology. 2009 Apr;56(5):922-8. https://pubmed.ncbi.nlm.nih.gov/19371583/
In vivo activity:
Mice were given SL327 before ethanol doses for acquisition or before post-conditioning tests for expression. SL327 significantly blocked the acquisition and expression of ethanol-induced CPP, but not CPA. Additionally, SL327 prevented the increase of pERK-positive neurons in specific brain regions. These findings suggest MEK's differential involvement in distinct behavioral outcomes during drug-induced place conditioning, influenced by the motivational sign and learning phase. Reference: J Psychopharmacol. 2017 Jan;31(1):105-114. https://pubmed.ncbi.nlm.nih.gov/28072036/
Solvent mg/mL mM
Solubility
DMSO 10.0 29.82
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 335.35 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Du J, Dong Z, Tan L, Tan M, Zhang F, Zhang K, Pan G, Li C, Shi S, Zhang Y, Liu Y, Cui H. Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma. Front Cell Dev Biol. 2020 Dec 17;8:607757. doi: 10.3389/fcell.2020.607757. PMID: 33392197; PMCID: PMC7773826. 2. Chen-Roetling J, Li Z, Chen M, Awe OO, Regan RF. Heme oxygenase activity and hemoglobin neurotoxicity are attenuated by inhibitors of the MEK/ERK pathway. Neuropharmacology. 2009 Apr;56(5):922-8. doi: 10.1016/j.neuropharm.2009.01.022. Epub 2009 Feb 6. PMID: 19371583; PMCID: PMC2692087. 3. Michalak A, Wnorowski A, Berardinelli A, Zinato S, Rusinek J, Budzyńska B. Diazepam and SL-327 synergistically attenuate anxiety-like behaviours in mice - Possible hippocampal MAPKs specificity. Neuropharmacology. 2020 Dec 1;180:108302. doi: 10.1016/j.neuropharm.2020.108302. Epub 2020 Sep 12. PMID: 32931814. 4. Rosas M, Porru S, Longoni R, Spina L, Peana AT, Collu M, Acquas E. Differential effects of the MEK inhibitor SL327 on the acquisition and expression of ethanol-elicited conditioned place preference and aversion in mice. J Psychopharmacol. 2017 Jan;31(1):105-114. doi: 10.1177/0269881116675514. Epub 2016 Nov 15. PMID: 28072036.
In vitro protocol:
1. Du J, Dong Z, Tan L, Tan M, Zhang F, Zhang K, Pan G, Li C, Shi S, Zhang Y, Liu Y, Cui H. Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma. Front Cell Dev Biol. 2020 Dec 17;8:607757. doi: 10.3389/fcell.2020.607757. PMID: 33392197; PMCID: PMC7773826. 2. Chen-Roetling J, Li Z, Chen M, Awe OO, Regan RF. Heme oxygenase activity and hemoglobin neurotoxicity are attenuated by inhibitors of the MEK/ERK pathway. Neuropharmacology. 2009 Apr;56(5):922-8. doi: 10.1016/j.neuropharm.2009.01.022. Epub 2009 Feb 6. PMID: 19371583; PMCID: PMC2692087.
In vivo protocol:
1. Michalak A, Wnorowski A, Berardinelli A, Zinato S, Rusinek J, Budzyńska B. Diazepam and SL-327 synergistically attenuate anxiety-like behaviours in mice - Possible hippocampal MAPKs specificity. Neuropharmacology. 2020 Dec 1;180:108302. doi: 10.1016/j.neuropharm.2020.108302. Epub 2020 Sep 12. PMID: 32931814. 2. Rosas M, Porru S, Longoni R, Spina L, Peana AT, Collu M, Acquas E. Differential effects of the MEK inhibitor SL327 on the acquisition and expression of ethanol-elicited conditioned place preference and aversion in mice. J Psychopharmacol. 2017 Jan;31(1):105-114. doi: 10.1177/0269881116675514. Epub 2016 Nov 15. PMID: 28072036.
1: Chen B, Zhao C, Zhu H, Lu X, Liu H, Lu Q, Zhu T, Huang C. β-glucan, a specific immuno-stimulant, produces rapid antidepressant effects by stimulating ERK1/2-dependent synthesis of BDNF in the hippocampus. Eur J Pharmacol. 2023 Dec 15;961:176161. doi: 10.1016/j.ejphar.2023.176161. Epub 2023 Nov 7. PMID: 37939990. 2: Zhu H, Liu X, Wang X, Li Y, Ma F, Tan B, Zhou P, Fu F, Su R. Gβγ subunit inhibitor decreases DOM-induced head twitch response via the PLCβ/IP3/Ca2+/ERK and cAMP signaling pathways. Eur J Pharmacol. 2023 Oct 15;957:176038. doi: 10.1016/j.ejphar.2023.176038. Epub 2023 Aug 30. PMID: 37657742. 3: Jing X, Han C, Li Q, Li F, Zhang J, Jiang Q, Zhao F, Guo C, Chen J, Jiang T, Wang X, Chen Y, Huang C. IGF2BP3-EGFR-AKT axis promotes breast cancer MDA-MB-231 cell growth. Biochim Biophys Acta Mol Cell Res. 2023 Dec;1870(8):119542. doi: 10.1016/j.bbamcr.2023.119542. Epub 2023 Jul 18. PMID: 37474008. 4: Hwang Y, Park JH, Kim HC, Shin EJ. GABAB receptor activation alters astrocyte phenotype changes induced by trimethyltin via ERK signaling in the dentate gyrus of mice. Life Sci. 2023 Apr 15;319:121529. doi: 10.1016/j.lfs.2023.121529. Epub 2023 Feb 23. PMID: 36841471. 5: Nonaka M, Kanouchi H, Torii S, Nagano H, Kondo S, Fujii A, Nagano M, Takenaka S. Lactic acid induces HSPA1A expression through ERK1/2 activation. Biosci Biotechnol Biochem. 2023 Jan 24;87(2):191-196. doi: 10.1093/bbb/zbac192. PMID: 36441019. 6: Blaine AT, Miao Y, Yuan J, Palant S, Liu RJ, Zhang ZY, van Rijn RM. Exploration of beta-arrestin isoform signaling pathways in delta opioid receptor agonist-induced convulsions. Front Pharmacol. 2022 Aug 11;13:914651. doi: 10.3389/fphar.2022.914651. PMID: 36059958; PMCID: PMC9428791. 7: Sun X, Jiang X, Li X, Qi Z, Lu Y. Sulfuretin exerts anti-depressive effects in the lipopolysaccharide-induced depressive mouse models. Physiol Behav. 2022 Jun 1;250:113800. doi: 10.1016/j.physbeh.2022.113800. Epub 2022 Apr 5. PMID: 35395250. 8: Huang Q, Wang P, Liu H, Li M, Yue Y, Xu P. Inhibition of ERK1/2 regulates cognitive function by decreasing expression levels of PSD-95 in the hippocampus of CIH rats. Eur J Neurosci. 2022 Mar;55(6):1471-1482. doi: 10.1111/ejn.15635. Epub 2022 Mar 10. Erratum in: Eur J Neurosci. 2022 Apr;55(8):2047. doi: 10.1111/ejn.15667. PMID: 35243702. 9: Meenakshi PK, Mehrotra D, Nruthyathi N, Almeida-Filho D, Lee YS, Silva A, Balaji J. Novel measures of Morris water maze performance that use vector field maps to assess accuracy, uncertainty, and intention of navigational searches. Hippocampus. 2022 Apr;32(4):264-285. doi: 10.1002/hipo.23404. Epub 2022 Jan 13. PMID: 35025127; PMCID: PMC8921876. 10: Du J, Dong Z, Tan L, Tan M, Zhang F, Zhang K, Pan G, Li C, Shi S, Zhang Y, Liu Y, Cui H. Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma. Front Cell Dev Biol. 2020 Dec 17;8:607757. doi: 10.3389/fcell.2020.607757. PMID: 33392197; PMCID: PMC7773826. 11: Gabbert L, Dilling C, Meybohm P, Burek M. Deletion of Protocadherin Gamma C3 Induces Phenotypic and Functional Changes in Brain Microvascular Endothelial Cells In Vitro. Front Pharmacol. 2020 Nov 30;11:590144. doi: 10.3389/fphar.2020.590144. PMID: 33390965; PMCID: PMC7774295. 12: Hwang Y, Kim HC, Shin EJ. Enhanced neurogenesis is involved in neuroprotection provided by rottlerin against trimethyltin-induced delayed apoptotic neuronal damage. Life Sci. 2020 Dec 1;262:118494. doi: 10.1016/j.lfs.2020.118494. Epub 2020 Sep 28. PMID: 32991881. 13: Michalak A, Wnorowski A, Berardinelli A, Zinato S, Rusinek J, Budzyńska B. Diazepam and SL-327 synergistically attenuate anxiety-like behaviours in mice - Possible hippocampal MAPKs specificity. Neuropharmacology. 2020 Dec 1;180:108302. doi: 10.1016/j.neuropharm.2020.108302. Epub 2020 Sep 12. PMID: 32931814. 14: Martínez-Laorden E, Navarro-Zaragoza J, Milanés MV, Laorden ML, Almela P. Cardiac Protective Role of Heat Shock Protein 27 in the Stress Induced by Drugs of Abuse. Int J Mol Sci. 2020 May 21;21(10):3623. doi: 10.3390/ijms21103623. PMID: 32455528; PMCID: PMC7279295. 15: Yang JH, Sohn S, Kim S, Kim J, Oh JH, Ryu IS, Go BS, Choe ES. Repeated nicotine exposure increases the intracellular interaction between ERK-mGluR5 in the nucleus accumbens more in adult than adolescent rats. Addict Biol. 2021 Mar;26(2):e12913. doi: 10.1111/adb.12913. Epub 2020 Apr 27. PMID: 32339332. 16: Angara K, Pai EL, Bilinovich SM, Stafford AM, Nguyen JT, Li KX, Paul A, Rubenstein JL, Vogt D. Nf1 deletion results in depletion of the Lhx6 transcription factor and a specific loss of parvalbumin+ cortical interneurons. Proc Natl Acad Sci U S A. 2020 Mar 17;117(11):6189-6195. doi: 10.1073/pnas.1915458117. Epub 2020 Mar 2. PMID: 32123116; PMCID: PMC7084085. 17: Chuang HW, Wei IH, Lin FY, Li CT, Chen KT, Tsai MH, Huang CC. Roles of Akt and ERK in mTOR-Dependent Antidepressant Effects of Vanillic Acid. ACS Omega. 2020 Feb 13;5(7):3709-3716. doi: 10.1021/acsomega.9b04271. PMID: 32118186; PMCID: PMC7045503. 18: Lu J, Zhou H, Meng D, Zhang J, Pan K, Wan B, Miao Z. Tanshinone IIA Improves Depression-like Behavior in Mice by Activating the ERK-CREB-BDNF Signaling Pathway. Neuroscience. 2020 Mar 15;430:1-11. doi: 10.1016/j.neuroscience.2020.01.026. Epub 2020 Jan 31. PMID: 32014436. 19: Lee HR, Lee J, Kim HJ. Differential effects of MEK inhibitors on rat neural stem cell differentiation: Repressive roles of MEK2 in neurogenesis and induction of astrocytogenesis by PD98059. Pharmacol Res. 2019 Nov;149:104466. doi: 10.1016/j.phrs.2019.104466. Epub 2019 Sep 25. PMID: 31562895. 20: Oh JH, Nam TJ. Hydrophilic Glycoproteins of an Edible Green Alga Capsosiphon fulvescens Prevent Aging-Induced Spatial Memory Impairment by Suppressing GSK-3β-Mediated ER Stress in Dorsal Hippocampus. Mar Drugs. 2019 Mar 15;17(3):168. doi: 10.3390/md17030168. PMID: 30875947; PMCID: PMC6470841.