DMDHP, short for (±)-dimyristoyl-2,3-dimethylhydroxypropylamine, is a cationic lipid primarily studied for its role in lipid nanoparticle (LNP) formulations for nucleic acid delivery. Its bioactivity is centered around enhancing transfection efficiency and facilitating endosomal escape in non-viral gene delivery systems. In studies, DMDHP-containing LNPs demonstrated high mRNA delivery efficiency in vitro, with transfection rates exceeding 70% in HeLa and HEK293 cells when formulated with helper lipids such as DOPE and cholesterol. In vivo, DMDHP-formulated LNPs showed robust luciferase expression in mouse liver and spleen tissues following intravenous injection, with luminescence signals comparable to or surpassing those of standard ionizable lipids. Additionally, DMDHP exhibits low cytotoxicity at working concentrations (<10 μg/mL), making it suitable for therapeutic development involving mRNA, siRNA, or CRISPR-Cas delivery.
MedKoo Cat#: 465235
Name: DMDHP
CAS#: 189743-89-3 (iodide)
Chemical Formula: C36H72INO6
Exact Mass: 741.4404
Molecular Weight: 741.88
Elemental Analysis: C, 58.28; H, 9.78; I, 17.11; N, 1.89; O, 12.94
The following data is based on the product molecular weight 741.88 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
