JFN-75223 is a fluorogenic substrate for trypsin, cathepsin B, and cathepsin H. Upon enzymatic cleavage by trypsin, cathepsin B, or cathepsin H, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify trypsin, cathepsin B, and cathepsin H activity. JFN-75223 displays excitation/emission maxima of 340-360/440-460 nm, respectively. This product has no formal name at the moment. For the convenience of communication, a temporary code name was therefore proposed according to MedKoo Chemical Nomenclature (see web page: https://www.medkoo.com/page/naming).
MedKoo Cat#: 464503
Name: JFN-75223 HCl
CAS#: 70375-22-3 (hydrochloride)
Chemical Formula: C24H28ClN5O5
Exact Mass:
Molecular Weight: 501.97
Elemental Analysis: C, 57.43; H, 5.62; Cl, 7.06; N, 13.95; O, 15.94
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 1.0 | 2.00 | |
| DMSO | 1.0 | 2.00 |
The following data is based on the product molecular weight 501.97 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
