Synonym
Dinitolmide; Dinitrotoluamide; Zoalene; NSC 758213; NSC758213; NSC-758213;
IUPAC/Chemical Name
2-methyl-3,5-dinitrobenzamide
InChi Key
ZEFNOZRLAWVAQF-UHFFFAOYSA-N
InChi Code
InChI=1S/C8H7N3O5/c1-4-6(8(9)12)2-5(10(13)14)3-7(4)11(15)16/h2-3H,1H3,(H2,9,12)
SMILES Code
O=C(N)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1C
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Biological target:
Dinitolmide (Zoalene), a fodder additive for poultry, has anti-coccidial effect. Dinitolmide can be used to prevent infections induced by Eimeria, such as Eimeria tenella, Eimeria necatrix, Eimeria brunette, and so on.
|
Solvent |
mg/mL |
mM |
| Solubility |
| DMSO |
72.5 |
321.99 |
| Ethanol |
1.0 |
4.44 |
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
225.16
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
Formulation protocol:
TBD
1: Liu J, Song S, Wu A, Wu X, Xiao J, Xu C. Development of a gold nanoparticle- based lateral-flow strip for the detection of dinitolmide in chicken tissue. Anal Methods. 2020 Jul 2;12(25):3210-3217. doi: 10.1039/d0ay00885k. PMID: 32930183.
2: Qu D, Ma W, Ye Y, Han J. Effect of dinitolmide intercalated into Montmorillonite on E. tenella infection in chickens. Parasitol Res. 2014 Mar;113(3):1233-8. doi: 10.1007/s00436-014-3762-7. Epub 2014 Jan 31. PMID: 24481902.
3: Zhao X, Wang B, Xie K, Liu Y, Zhang Y, Wang Y, Liu C, Guo Y, Bu X, Zhang G, Zhang T, Dai G. Development of an ASE-GC-MS/MS method for detecting dinitolmide and its metabolite 3-ANOT in eggs. J Mass Spectrom. 2018 Oct;53(10):976-985. doi: 10.1002/jms.4267. Epub 2018 Aug 15. PMID: 29996008.
4: Sun XY, Bi SY, Wu J, Zhao R. Study on the interaction of amprolium HCl and dinitolmide in animal-derived food products with BSA by multiple spectroscopies and molecular modeling techniques. J Biomol Struct Dyn. 2019 Oct;37(16):4283-4291. doi: 10.1080/07391102.2018.1548978. Epub 2019 Jan 13. PMID: 30517053.
5: Reece RL, Hooper PT. Toxicity in utility pigeons caused by the coccidiostat dinitolmide. Aust Vet J. 1984 Aug;61(8):259-61. doi: 10.1111/j.1751-0813.1984.tb15537.x. PMID: 6508669.
6: Joyner LP, Norton CC. The anticoccidial effects of amprolium, dinitolmide and monensin against Eimeria maxima, E, brunetti and E. acervulina with particular reference to oocyst sporulation. Parasitology. 1977 Oct;75(2):155-64. doi: 10.1017/s0031182000062296. PMID: 927884.
7: B S, Preetam VC, Srinivas G, Rao DS. Effect of Cocciban herbal coccidiostats on hematobiochemical, fecal parameters and cecal histopathology of broiler chicken. Trop Anim Health Prod. 2019 Jul;51(6):1375-1381. doi: 10.1007/s11250-019-01831-3. Epub 2019 Feb 7. PMID: 30729387.
8: Mathis GF, McDougald LR. Effect of amprolium and dinitolmide on sporulation of oocysts of field isolates of Eimeria acervulina. Parasitology. 1981 Oct;83(Pt 2):281-4. doi: 10.1017/s0031182000085292. PMID: 7329718.
9: Srinivasu B, Preetam VC, Gurram S, Reddy AR. Comparative evaluation of herbal coccidiostat with chemotherapeutic coccidiostats on performance of broilers to control coccidiosis. Trop Anim Health Prod. 2020 Jul;52(4):1985-1989. doi: 10.1007/s11250-020-02220-x. Epub 2020 Jan 24. PMID: 31981055.
10: Muharem M, Yan H, Xu S, Feng N, Hao J, Zhu C, Guo S, Zhang Z, Han N. [Determination of six anticoccidials in chicken using QuEChERS combined with ultra high liquid chromatography-high resolution mass spectrometry]. Se Pu. 2015 Nov;33(11):1199-204. Chinese. doi: 10.3724/sp.j.1123.2015.06016. PMID: 26939367.
