FLTX1 | CAS#1481401-71-1 | fluorescent Tamoxifen derivative

MedKoo Cat#: 555964 | Name: FLTX1

Description:

WARNING: This product is for research use only, not for human or veterinary use.

FLTX1 is a fluorescent Tamoxifen derivative that can specifically label intracellular Tamoxifen-binding sites (estrogen receptors). FLTX1 allows the labeling of estrogen receptors in immunocytochemistry and immunohistochemistry studies, both under permeabilized and non-permeabilized conditions. FLTX1 showed the pharmacological activity of the tamoxifen moiety and efficient fluorescence properties, which could be used synergistically to improve the effect of the drug.

Chemical Structure

FLTX1

CAS#1481401-71-1

Theoretical Analysis

MedKoo Cat#: 555964

Name: FLTX1

CAS#: 1481401-71-1

Chemical Formula: C31H28N4O4

Exact Mass: 520.2111

Molecular Weight: 520.59

Elemental Analysis: C, 71.52; H, 5.42; N, 10.76; O, 12.29

Price and Availability

Size	Price	Availability
5mg	USD 150.00	Ready to ship
10mg	USD 250.00	Ready to ship
25mg	USD 450.00	Ready to ship
50mg	USD 750.00	Ready to ship
100mg	USD 1,250.00	Ready to ship
200mg	USD 2,050.00	Ready to ship

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Related CAS #

No Data

Synonym

FLTX1; FLTX-1; FLTX 1; CB-38231393; CB38231393; CB 38231393;

IUPAC/Chemical Name

N-[2-[4-[(1Z)-1,2-Diphenyl-1-buten-1-yl]phenoxy]ethyl]-N-methyl-7-nitro-2,1,3-benzoxadiazol-4-amine

InChi Key

UKSXWLGSDIHAEK-WCTVFOPTSA-N

InChi Code

InChI=1S/C31H28N4O4/c1-3-26(22-10-6-4-7-11-22)29(23-12-8-5-9-13-23)24-14-16-25(17-15-24)38-21-20-34(2)27-18-19-28(35(36)37)31-30(27)32-39-33-31/h4-19H,3,20-21H2,1-2H3/b29-26-

SMILES Code

O=[N+](C1=CC=C(N(CCOC2=CC=C(/C(C3=CC=CC=C3)=C(C4=CC=CC=C4)/CC)C=C2)C)C5=NON=C51)[O-]

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Certificate of Analysis

View CoA: current batch, Lot#T23D03P22

QC Data

View QC data: current batch, Lot#T23D03P22

Safety Data Sheet (SDS)

View Safety Data Sheet (SDS)

Instruction

View handling instruction

Biological target:

FLTX1 is a fluorescent Tamoxifen derivative that can specifically label intracellular Tamoxifen-binding sites (estrogen receptors) under permeabilized and non-permeabilized conditions.

In vitro activity:

Ligand binding assays showed that FLTX1 exhibits similar affinity for ER than tamoxifen. FLTX1 exhibited antiestrogenic activity comparable to tamoxifen in MCF7 and T47D cells transfected with 3xERE-luciferase reporter. Reference: Eur J Pharm Biopharm. 2013 Nov;85(3 Pt B):898-910. https://pubmed.ncbi.nlm.nih.gov/23727370/

In vivo activity:

In the rat uterine model of estrogenicity/antiestrogenicity, FLTX1 displayed antagonistic activity comparable to tamoxifen at lower doses, and only estrogenic uterotrophy at the highest dose. Reference: Eur J Pharm Biopharm. 2013 Nov;85(3 Pt B):898-910. https://pubmed.ncbi.nlm.nih.gov/23727370/

	Solvent	mg/mL	mM
Solubility
	DMSO	25.0	48.02

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 520.59 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

Marrero-Alonso J, Morales A, García Marrero B, Boto A, Marín R, Cury D, Gómez T, Fernández-Pérez L, Lahoz F, Díaz M. Unique SERM-like properties of the novel fluorescent tamoxifen derivative FLTX1. Eur J Pharm Biopharm. 2013 Nov;85(3 Pt B):898-910. doi: 10.1016/j.ejpb.2013.04.024. Epub 2013 May 31. PMID: 23727370.

In vitro protocol:

In vivo protocol:

1: Morales A, Marín R, Marrero-Alonso J, Boto A, Díaz M. Colocalization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal Microscopy. Methods Mol Biol. 2016;1366:163-173. doi: 10.1007/978-1-4939-3127-9_13. PMID: 26585134. 2: Marrero-Alonso J, Morales A, García Marrero B, Boto A, Marín R, Cury D, Gómez T, Fernández-Pérez L, Lahoz F, Díaz M. Unique SERM-like properties of the novel fluorescent tamoxifen derivative FLTX1. Eur J Pharm Biopharm. 2013 Nov;85(3 Pt B):898-910. doi: 10.1016/j.ejpb.2013.04.024. Epub 2013 May 31. PMID: 23727370. 3: Lahoz F, Scholz LE, Boto A, Díaz M. FRET mechanism between a fluorescent breast-cancer drug and photodynamic therapy sensitizers. Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 5;239:118498. doi: 10.1016/j.saa.2020.118498. Epub 2020 May 19. PMID: 32470814. 4: Díaz M, Lobo F, Hernández D, Amesty Á, Valdés-Baizabal C, Canerina-Amaro A, Mesa-Herrera F, Soler K, Boto A, Marín R, Estévez-Braun A, Lahoz F. FLTX2: A Novel Tamoxifen Derivative Endowed with Antiestrogenic, Fluorescent, and Photosensitizer Properties. Int J Mol Sci. 2021 May 19;22(10):5339. doi: 10.3390/ijms22105339. PMID: 34069498; PMCID: PMC8161337.