HOE 33342, also known as Hoechst 33342, HO342, is a Benzimidazole fluorescent dye and a Cell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. HOE 33342 trihydrochloride is used to quantify DNA in viable cells.
MedKoo Cat#: 576326
Name: HOE-33342 free base
CAS#: 23491-52-3 (free base)
Chemical Formula: C27H28N6O
Exact Mass: 452.2325
Molecular Weight: 452.56
Elemental Analysis: C, 71.66; H, 6.24; N, 18.57; O, 3.54
The following data is based on the product molecular weight 452.56 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
