|
Solvent |
mg/mL |
mM |
| Solubility |
| To be determined |
0.0 |
0.00 |
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
255.27
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: HC Blue No. 1. IARC Monogr Eval Carcinog Risks Hum. 1993;57:129-42. PMID: 8207849; PMCID: PMC7682267.
2: Abu-Shakra A, Johnson L, Earley K, Jameson CW, Kari FW, Gupta R, Langenbach R. Isolation of the mutagenic and DNA adduct-inducing components from a commercial preparation of HC blue 1 using Salmonella (TA98) bioassay-directed HPLC fractionation. Mutat Res. 1991 Aug;260(4):377-85. doi: 10.1016/0165-1218(91)90023-f. PMID: 1870626.
3: Hill LE, Parton JW, Probst GS, Garriott ML. Mutagenicity evaluation of HC Blue No. 1 and HC Blue No. 2, II. Effect on the in vitro induction of unscheduled DNA synthesis in rat, mouse, rabbit, hamster, and monkey primary hepatocytes. Mutat Res. 1990 Jun;241(2):145-50. doi: 10.1016/0165-1218(90)90118-l. PMID: 2345554.
4: Parton JW, Beyers JE, Garriott ML. Mutagenicity evaluation of HC Blue No. 1 and HC Blue No. 2, I. Effect on the induction of micronuclei in mouse bone marrow cells. Mutat Res. 1990 Jun;241(2):139-44. doi: 10.1016/0165-1218(90)90117-k. PMID: 2345553.
5: Oberly TJ, Kokkino AJ, Bewsey BJ, Richardson KK. Mutagenicity evaluation of HC Blue No. 1 and HC Blue No. 2. III. Effects in the Salmonella typhimurium/Escherichia coli reversion assay and the mouse lymphoma L5178Y TK+/- forward mutation assay. Mutat Res. 1990 Jun;241(2):151-9. doi: 10.1016/0165-1218(90)90119-m. PMID: 2189067.
6: Kari FW, Driscoll SM, Abu-Shakra A, Strom SC, Jenkins WL, Volosin JS, Rudo KM, Langenbach R. Comparative metabolism and genotoxicity of the structurally similar nitrophenylenediamine dyes, HC Blue 1 and HC Blue 2, in mouse hepatocytes. Cell Biol Toxicol. 1990 Apr;6(2):139-55. doi: 10.1007/BF00249590. PMID: 2361189.
7: Kari F, Driscoll S, Parker C, Rudo K, Tomer K, Langenbach R. Species comparisons regarding comparative metabolism of two structurally similar phenylenediamines (HC Blue 1 and HC Blue 2). Prog Clin Biol Res. 1990;340D:305-14. PMID: 2371302.
8: Kari FW, Mennear JH, Farnell D, Thompson RB, Huff JE. Comparative carcinogenicity of two structurally similar phenylenediamine dyes (HC blue no. 1 and HC blue no. 2) in F344/N rats and B6C3F1 mice. Toxicology. 1989 Jun 1;56(2):155-65. doi: 10.1016/0300-483x(89)90130-3. PMID: 2734800.
9: National Toxicology Program. NTP Toxicology and Carcinogenesis Studies of C.I. Acid Orange 3 (CAS No. 6373-74-6) in F344/N Rats and B6C3F1 Mice (Gavage Studies). Natl Toxicol Program Tech Rep Ser. 1988 Dec;335:1-157. PMID: 12732901.
10: Burnett CM, Corbett JF. Failure of short-term in vitro mutagenicity tests to predict the animal carcinogenicity of hair dyes. Food Chem Toxicol. 1987 Sep;25(9):703-7. doi: 10.1016/0278-6915(87)90104-9. PMID: 3653824.
11: National Toxicology Program. NTP Toxicology and Carcinogenesis Studies of HC Blue No. 1 (CAS No. 2784-94-3) in F344/N Rats and B6C3F1 Mice (Feed Studies). Natl Toxicol Program Tech Rep Ser. 1985 Aug;271:1-192. PMID: 12748683.
12: Darroudi F, Sobels FH, Natarajan AT. Evaluation of the mutagenic potential of the hair dye N-methyl-amino-2-nitro-4-N',N'-bis-(2-hydroxyethyl)-aminobenzene in a battery consisting of Drosophila and mammalian cytogenetic assays. Mutat Res. 1983 Feb;116(2):169-78. doi: 10.1016/0165-1218(83)90108-8. PMID: 6402694.
13: Loprieno N, Mariani L, Rusciano D. Lack of genotoxic properties of the hair- dye component N-methyl-amino-2-nitro-4-N',N'-bis-(2-hydroxyethyl)-aminobenzene, in mammalian cells in vitro, and in yeasts. Mutat Res. 1983 Feb;116(2):161-8. doi: 10.1016/0165-1218(83)90107-6. PMID: 6402693.
14: Shahin MM, Bugaut A. Mutagenic evaluation of the monocyclic aromatic amine N-methylamino-2-nitro-4-N', N'-bis(2-hydroxyethyl)-aminobenzene in the Salmonella typhimurium/mammalian microsome test. Mutat Res. 1983 Feb;116(2):155-9. doi: 10.1016/0165-1218(83)90106-4. PMID: 6338359.
