Trypan Blue | CAS#72-57-1 | Azo dye

MedKoo Cat#: 464021 | Name: Trypan Blue

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Trypan blue is an azo dye. It is a direct dye for cotton textiles. In biosciences, it is used as a vital stain to selectively color dead tissues or cells blue. Live cells or tissues with intact cell membranes are not colored. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells appear as a distinctive blue color under a microscope. Since live cells are excluded from staining, this staining method is also described as a dye exclusion method.

Chemical Structure

Trypan Blue

CAS#72-57-1

Theoretical Analysis

MedKoo Cat#: 464021

Name: Trypan Blue

CAS#: 72-57-1

Chemical Formula: C34H24N6Na4O14S4

Exact Mass: 959.9824

Molecular Weight: 960.79

Elemental Analysis: C, 42.50; H, 2.52; N, 8.75; Na, 9.57; O, 23.31; S, 13.35

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.

Bulk Inquiry

Related CAS #

No Data

Synonym

Trypan Blue; NSC 11247; NSC11247; NSC-11247;

IUPAC/Chemical Name

sodium 3,3'-((1E,1'E)-(3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(diazene-2,1-diyl))bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)

InChi Key

GLNADSQYFUSGOU-GPTZEZBUSA-J

InChi Code

InChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;

SMILES Code

Cc1c(/N=N/c(c2O)c(S(=O)([O-])=O)cc3c2c(N)cc(S(=O)([O-])=O)c3)ccc(c4cc(C)c(/N=N/c(c5O)c(S(=O)([O-])=O)cc6c5c(N)cc(S(=O)([O-])=O)c6)cc4)c1.[Na+].[Na+].[Na+].[Na+]

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Instruction

View handling instruction

	Solvent	mg/mL	mM
Solubility
	Soluble in DMSO	0.0	100.00

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 960.79 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

1: Rezano A, Ridhayanti F, Rangkuti AR, Gunawan T, Winarno GNA, Wijaya I. Cytotoxicity of Simvastatin in Human Breast Cancer MCF-7 and MDA-MB-231 Cell Lines. Asian Pac J Cancer Prev. 2021 Feb 1;22(S1):33-42. doi: 10.31557/APJCP.2021.22.S1.33. PMID: 33576210. 2: Cheng KC, Hsu YT, Liu W, Huang HL, Chen LY, He CX, Sheu SJ, Chen KJ, Lee PY, Lin YH, Chiu CC. The Role of Oxidative Stress and Autophagy in Blue-Light- Induced Damage to the Retinal Pigment Epithelium in Zebrafish In Vitro and In Vivo. Int J Mol Sci. 2021 Jan 29;22(3):1338. doi: 10.3390/ijms22031338. PMID: 33572787. 3: Hendawy H, Uemura A, Ma D, Namiki R, Samir H, Ahmed MF, Elfadadny A, El- Husseiny HM, Chieh-Jen C, Tanaka R. Tissue Harvesting Site Effect on the Canine Adipose Stromal Vascular Fraction Quantity and Quality. Animals (Basel). 2021 Feb 9;11(2):460. doi: 10.3390/ani11020460. PMID: 33572472. 4: Sadžak A, Vlašić I, Kiralj Z, Batarelo M, Oršolić N, Jazvinšćak Jembrek M, Kušen I, Šegota S. Neurotoxic Effect of Flavonol Myricetin in the Presence of Excess Copper. Molecules. 2021 Feb 5;26(4):845. doi: 10.3390/molecules26040845. PMID: 33562817. 5: Rockinger U, Müller C, Bracher F, Funk M, Winter G. DMSO as new, counterintuitive excipient for freeze-drying human keratinocytes. Eur J Pharm Sci. 2021 Feb 6:105746. doi: 10.1016/j.ejps.2021.105746. Epub ahead of print. PMID: 33561511. 6: Mihanfar A, Darband SG, Sadighparvar S, Kaviani M, Mirza-Aghazadeh-Attari M, Yousefi B, Majidinia M. In vitro and in vivo anticancer effects of syringic acid on colorectal cancer: Possible mechanistic view. Chem Biol Interact. 2021 Feb 4;337:109337. doi: 10.1016/j.cbi.2020.109337. Epub ahead of print. PMID: 33548266. 7: Figueiredo N, Matos B, Diniz M, Branco V, Martins M. Marine Fish Primary Hepatocyte Isolation and Culture: New Insights to Enzymatic Dissociation Pancreatin Digestion. Int J Environ Res Public Health. 2021 Feb 3;18(4):1380. doi: 10.3390/ijerph18041380. PMID: 33546159. 8: Prakash R, Mishra RK, Ahmad A, Khan MA, Khan R, Raza SS. Sivelestat-loaded nanostructured lipid carriers modulate oxidative and inflammatory stress in human dental pulp and mesenchymal stem cells subjected to oxygen-glucose deprivation. Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111700. doi: 10.1016/j.msec.2020.111700. Epub 2020 Nov 6. PMID: 33545859. 9: Ní Dhubhghaill S, Miron A, Lie JT, Dapena I, Oellerich S, Melles GRJ. Preclinical testing of small diameter Descemet membrane endothelial keratoplasty grafts to increase tissue availability. PLoS One. 2021 Feb 4;16(2):e0246516. doi: 10.1371/journal.pone.0246516. PMID: 33539395. 10: Ombredane AS, de Andrade LR, Bonadio RS, Pinheiro WO, de Azevedo RB, Joanitti GA. Melittin sensitizes skin squamous carcinoma cells to 5-fluorouracil by affecting cell proliferation and survival. Exp Dermatol. 2021 Feb 1. doi: 10.1111/exd.14289. Epub ahead of print. PMID: 33523510. 11: Wu CH, Chen CH, Hsieh PF, Lee YH, Kuo WW, Wu RC, Hung CH, Yang YL, Lin VC. Verbascoside inhibits the epithelial-mesenchymal transition of prostate cancer cells through high-mobility group box 1/receptor for advanced glycation end- products/TGF-β pathway. Environ Toxicol. 2021 Feb 1. doi: 10.1002/tox.23107. Epub ahead of print. PMID: 33522686. 12: Stan MS, Badea S, Hermenean A, Herman H, Trica B, Sbarcea BG, Dinischiotu A. New Insights into the Cell Death Signaling Pathways Triggered by Long-Term Exposure to Silicon-Based Quantum Dots in Human Lung Fibroblasts. Nanomaterials (Basel). 2021 Jan 27;11(2):323. doi: 10.3390/nano11020323. PMID: 33513804. 13: Rajendran NK, Houreld NN, Abrahamse H. In Vitro Wound Healing Potential of Photobiomodulation Is Possibly Mediated by Its Stimulatory Effect on AKT Expression in Adipose-Derived Stem Cells. Oxid Med Cell Longev. 2021 Jan 9;2021:6664627. doi: 10.1155/2021/6664627. PMID: 33505585; PMCID: PMC7811432. 14: Baniasadi F, Hajiaghalou S, Shahverdi A, Pirhajati V, Fathi R. Static magnetic field halves cryoinjuries of vitrified mouse COCs, improves their functions and modulates pluripotency of derived blastocysts. Theriogenology. 2021 Jan 3;163:31-42. doi: 10.1016/j.theriogenology.2020.12.025. Epub ahead of print. PMID: 33503549. 15: Morais M, Machado V, Dias F, Palmeira C, Martins G, Fonseca M, Martins CSM, Teixeira AL, Prior JAV, Medeiros R. Starch-Capped AgNPs' as Potential Cytotoxic Agents against Prostate Cancer Cells. Nanomaterials (Basel). 2021 Jan 20;11(2):E256. doi: 10.3390/nano11020256. PMID: 33498166. 16: Gowri M, Latha N, Suganya K, Murugan M, Rajan M. Calcium alginate nanoparticle crosslinked phosphorylated polyallylamine to the controlled release of clindamycin for osteomyelitis treatment. Drug Dev Ind Pharm. 2021 Feb 1:1-12. doi: 10.1080/03639045.2021.1879835. Epub ahead of print. PMID: 33493022. 17: Pardede BP, Agil M, Yudi Y, Supriatna I. Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross Breeding Program. Vet World. 2020 Dec;13(12):2649-2657. doi: 10.14202/vetworld.2020.2649-2657. Epub 2020 Dec 14. PMID: 33487983; PMCID: PMC7811557. 18: Bumah VV, Masson-Meyers DS, Awosika O, Zacharias S, Enwemeka CS. The viability of human cells irradiated with 470-nm light at various radiant energies in vitro. Lasers Med Sci. 2021 Jan 24. doi: 10.1007/s10103-021-03250-z. Epub ahead of print. PMID: 33486613. 19: Khamesipour F, Razavi SM, Hejazi SH, Ghanadian SM. In vitro and in vivo Anti-Toxoplasma activity of Dracocephalum kotschyi essential oil. Food Sci Nutr. 2020 Nov 12;9(1):522-531. doi: 10.1002/fsn3.2021. PMID: 33473313; PMCID: PMC7802582. 20: Wang Y, Nguyen DT, Yang G, Anesi J, Kelly J, Chai Z, Ahmady F, Charchar F, Golledge J. A Modified MTS Proliferation Assay for Suspended Cells to Avoid the Interference by Hydralazine and β-Mercaptoethanol. Assay Drug Dev Technol. 2021 Jan 20. doi: 10.1089/adt.2020.1027. Epub ahead of print. PMID: 33471568.