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MedKoo Cat#: 575591 | Name: Fluorescein diester

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Fluorescein diester is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.

Chemical Structure

Fluorescein diester
Fluorescein diester
CAS#75296-16-1

Theoretical Analysis

MedKoo Cat#: 575591

Name: Fluorescein diester

CAS#: 75296-16-1

Chemical Formula: C44H32N8O9S

Exact Mass: 848.2013

Molecular Weight: 848.85

Elemental Analysis: C, 62.26; H, 3.80; N, 13.20; O, 16.96; S, 3.78

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.
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Related CAS #
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Synonym
Fluorescein diester
IUPAC/Chemical Name
Benzoic acid, 4-((aminoiminomethyl)amino)-, 5-((((4-carboxyphenyl)amino)thioxomethyl)amino)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-3',6'-diyl ester
InChi Key
LYWNSVUNJBBQBQ-UHFFFAOYSA-N
InChi Code
InChI=1S/C44H32N8O9S/c45-41(46)49-25-9-3-23(4-10-25)38(55)58-29-14-17-33-35(20-29)60-36-21-30(59-39(56)24-5-11-26(12-6-24)50-42(47)48)15-18-34(36)44(33)32-16-13-28(19-31(32)40(57)61-44)52-43(62)51-27-7-1-22(2-8-27)37(53)54/h1-21H,(H,53,54)(H4,45,46,49)(H4,47,48,50)(H2,51,52,62)
SMILES Code
C1=CC(=CC=C1C(=O)O)NC(=S)NC2=CC3=C(C=C2)C4(C5=C(C=C(C=C5)OC(=O)C6=CC=C(C=C6)NC(=N)N)OC7=C4C=CC(=C7)OC(=O)C8=CC=C(C=C8)NC(=N)N)OC3=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 848.85 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1. Zhang L, Zhang X. A selectively fluorescein-based colorimetric probe for detecting copper(II) ion. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Dec 10;133:54-9. doi: 10.1016/j.saa.2014.04.130. Epub 2014 Apr 30. PMID: 24929315. 2. Ahn B, Rhee SG, Stadtman ER. Use of fluorescein hydrazide and fluorescein thiosemicarbazide reagents for the fluorometric determination of protein carbonyl groups and for the detection of oxidized protein on polyacrylamide gels. Anal Biochem. 1987 Mar;161(2):245-57. doi: 10.1016/0003-2697(87)90448-9. PMID: 2883911. 3. Zhang J, Zhang L, Wei Y, Ma J, Shuang S, Cai Z, Dong C. A selectively fluorescein-based colorimetric probe for detecting copper(II) ion. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Mar 25;122:731-6. doi: 10.1016/j.saa.2013.11.096. Epub 2013 Nov 23. PMID: 24370938.