pNZ-TMP is a TMP prodrug, and is the first small-molecule approach capable of detecting and controlling engineered cell-cell outputs. pNZ-TMP is also sterically “caged” TMP with 27-fold reduced binding affinity for reporter-DD protein fusions in dose response assays. The “caged” prodrug is enzymatically liberated by an activator-cell, restoring high affinity for the protein target, and can diffuse into the surrounding medium as shown by a media transfer experiment. If the liberated drug reaches a nearby receiver-cell in sufficient concentration, it can stabilize DD fusion proteins eliciting a protein signal output, thereby reporting an interaction.
MedKoo Cat#: 463715
Name: pNZ-TMP
CAS#: 2759920-03-9
Chemical Formula: C22H23N5O7
Exact Mass: 469.1597
Molecular Weight: 469.45
Elemental Analysis: C, 56.29; H, 4.94; N, 14.92; O, 23.86
The following data is based on the product molecular weight 469.45 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
