Synonym
Sodium Ascorbate; Vitamin C sodium; Ascorbicin; Sodium L-ascorbate; L-Ascorbic Acid Sodium Salt
IUPAC/Chemical Name
sodium (R)-2-((S)-1,2-dihydroxyethyl)-4-hydroxy-5-oxo-2,5-dihydrofuran-3-olate
InChi Key
PPASLZSBLFJQEF-RXSVEWSESA-M
InChi Code
InChI=1S/C6H8O6.Na/c7-1-2(8)5-3(9)4(10)6(11)12-5;/h2,5,7-10H,1H2;/q;+1/p-1/t2-,5+;/m0./s1
SMILES Code
OC[C@@H]([C@H]1OC(C(O)=C1[O-])=O)O.[Na+]
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO and water
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Biological target:
Sodium ascorbate selectively inhibits Cav3.2 channels with an IC50 of 6.5 μM.
In vitro activity:
This study suggests that further clinical trials are warranted for advanced/refractory acute myeloid leukemia and acute promyelocytic leukemia since high plasma sodium ascorbate concentrations can be safely reached intravenously. High sodium ascorbate doses (0.5 to 7 mM) on various human myeloid cell lines revealed high sensitivity, especially in AML-derived lines. Normal cord blood CD34+ cells showed no significant cytotoxic effects.
Reference: Ann Hematol. 2015 Nov;94(11):1807-16. https://pubmed.ncbi.nlm.nih.gov/26264692/
In vivo activity:
The results of this study suggest that sodium ascorbate acts as a pure promoter but not a complete carcinogen in rats. In bladder-cancer-susceptible transgenic rats, sodium ascorbate treatment did not show a significantly higher incidence of carcinoma compared to control. The total bladder tumor incidences, including papillomas, also did not differ statistically.
Reference: Toxicol Pathol. 2005;33(7):764-7. https://pubmed.ncbi.nlm.nih.gov/16316941/
|
Solvent |
mg/mL |
mM |
comments |
Solubility |
DMSO |
1.0 |
5.05 |
|
Water |
100.0 |
504.77 |
|
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
198.11
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
Formulation protocol:
1. Mastrangelo D, Massai L, Lo Coco F, Noguera NI, Borgia L, Fioritoni G, Berardi A, Iacone A, Muscettola M, Pelosi E, Castelli G, Testa U, Di Pisa F, Grasso G. Cytotoxic effects of high concentrations of sodium ascorbate on human myeloid cell lines. Ann Hematol. 2015 Nov;94(11):1807-16. doi: 10.1007/s00277-015-2464-2. Epub 2015 Aug 12. PMID: 26264692.
2. Carosio R, Zuccari G, Orienti I, Mangraviti S, Montaldo PG. Sodium ascorbate induces apoptosis in neuroblastoma cell lines by interfering with iron uptake. Mol Cancer. 2007 Aug 30;6:55. doi: 10.1186/1476-4598-6-55. PMID: 17760959; PMCID: PMC2000471.
3. Ryszawy D, Pudełek M, Catapano J, Ciarach M, Setkowicz Z, Konduracka E, Madeja Z, Czyż J. High doses of sodium ascorbate interfere with the expansion of glioblastoma multiforme cells in vitro and in vivo. Life Sci. 2019 Sep 1;232:116657. doi: 10.1016/j.lfs.2019.116657. Epub 2019 Jul 13. PMID: 31306660.
4. Morimura K, Kang JS, Wei M, Wanibuchi H, Tsuda H, Fukushima S. Lack of urinary bladder carcinogenicity of sodium L-ascorbate in human c-Ha-ras proto-oncogene transgenic rats. Toxicol Pathol. 2005;33(7):764-7. doi: 10.1080/01926230500416336. PMID: 16316941.
In vitro protocol:
1. Mastrangelo D, Massai L, Lo Coco F, Noguera NI, Borgia L, Fioritoni G, Berardi A, Iacone A, Muscettola M, Pelosi E, Castelli G, Testa U, Di Pisa F, Grasso G. Cytotoxic effects of high concentrations of sodium ascorbate on human myeloid cell lines. Ann Hematol. 2015 Nov;94(11):1807-16. doi: 10.1007/s00277-015-2464-2. Epub 2015 Aug 12. PMID: 26264692.
2. Carosio R, Zuccari G, Orienti I, Mangraviti S, Montaldo PG. Sodium ascorbate induces apoptosis in neuroblastoma cell lines by interfering with iron uptake. Mol Cancer. 2007 Aug 30;6:55. doi: 10.1186/1476-4598-6-55. PMID: 17760959; PMCID: PMC2000471.
In vivo protocol:
1. Ryszawy D, Pudełek M, Catapano J, Ciarach M, Setkowicz Z, Konduracka E, Madeja Z, Czyż J. High doses of sodium ascorbate interfere with the expansion of glioblastoma multiforme cells in vitro and in vivo. Life Sci. 2019 Sep 1;232:116657. doi: 10.1016/j.lfs.2019.116657. Epub 2019 Jul 13. PMID: 31306660.
2. Morimura K, Kang JS, Wei M, Wanibuchi H, Tsuda H, Fukushima S. Lack of urinary bladder carcinogenicity of sodium L-ascorbate in human c-Ha-ras proto-oncogene transgenic rats. Toxicol Pathol. 2005;33(7):764-7. doi: 10.1080/01926230500416336. PMID: 16316941.
1: Tóth SZ, Lőrincz T, Szarka A. Concentration Does Matter: The Beneficial and Potentially Harmful Effects of Ascorbate in Humans and Plants. Antioxid Redox Signal. 2018 Nov 20;29(15):1516-1533. doi: 10.1089/ars.2017.7125. Epub 2017 Dec 1. PubMed PMID: 28974112.
2: Zechmann B. Compartment-Specific Importance of Ascorbate During Environmental Stress in Plants. Antioxid Redox Signal. 2018 Nov 20;29(15):1488-1501. doi: 10.1089/ars.2017.7232. Epub 2017 Aug 10. PubMed PMID: 28699398.
3: Tovmasyan A, Bueno-Janice JC, Jaramillo MC, Sampaio RS, Reboucas JS, Kyui N, Benov L, Deng B, Huang TT, Tome ME, Spasojevic I, Batinic-Haberle I. Radiation-Mediated Tumor Growth Inhibition Is Significantly Enhanced with Redox-Active Compounds That Cycle with Ascorbate. Antioxid Redox Signal. 2018 Nov 1;29(13):1196-1214. doi: 10.1089/ars.2017.7218. Epub 2018 Mar 27. PubMed PMID: 29390861; PubMed Central PMCID: PMC6157436.
4: Mi DJ, Dixit S, Warner TA, Kennard JA, Scharf DA, Kessler ES, Moore LM, Consoli DC, Bown CW, Eugene AJ, Kang JQ, Harrison FE. Altered glutamate clearance in ascorbate deficient mice increases seizure susceptibility and contributes to cognitive impairment in APP/PSEN1 mice. Neurobiol Aging. 2018 Nov;71:241-254. doi: 10.1016/j.neurobiolaging.2018.08.002. Epub 2018 Aug 7. PubMed PMID: 30172223; PubMed Central PMCID: PMC6162152.
5: Alexander MS, O'Leary BR, Wilkes JG, Gibson AR, Wagner BA, Du J, Sarsour E, Hwang RF, Buettner GR, Cullen JJ. Enhanced Pharmacological Ascorbate Oxidation Radiosensitizes Pancreatic Cancer. Radiat Res. 2018 Oct 30. doi: 10.1667/RR15189.1. [Epub ahead of print] PubMed PMID: 30376411.