ID-8 inhibitor | CAS#147591-46-6 | DYRK inhibitor

MedKoo Cat#: 555860 | Name: ID-8 inhibitor

Description:

WARNING: This product is for research use only, not for human or veterinary use.

ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.

Chemical Structure

ID-8 inhibitor

CAS#147591-46-6

Theoretical Analysis

MedKoo Cat#: 555860

Name: ID-8 inhibitor

CAS#: 147591-46-6

Chemical Formula: C16H14N2O4

Exact Mass: 298.0954

Molecular Weight: 298.30

Elemental Analysis: C, 64.42; H, 4.73; N, 9.39; O, 21.45

Price and Availability

Size	Price	Availability
50mg	USD 450.00	2 Weeks
100mg	USD 750.00	2 Weeks
200mg	USD 1,250.00	2 Weeks
500mg	USD 2,650.00	2 Weeks
1g	USD 3,650.00	2 Weeks
2g	USD 6,450.00	2 Weeks

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Related CAS #

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Synonym

ID-8 inhibitor; ID 8 inhibitor; ID8 inhibitor;

IUPAC/Chemical Name

1-(4-methoxyphenyl)-2-methyl-3-nitro-1H-indol-6-ol

InChi Key

VVZNWYXIOADGSW-UHFFFAOYSA-N

InChi Code

InChI=1S/C16H14N2O4/c1-10-16(18(20)21)14-8-5-12(19)9-15(14)17(10)11-3-6-13(22-2)7-4-11/h3-9,19H,1-2H3

SMILES Code

OC1=CC2=C(C=C1)C([N+]([O-])=O)=C(C)N2C3=CC=C(OC)C=C3

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

ID-8 is an inhibitor of dual-specificity tyrosine phosphorylation-regulated kinase (DYRK). ID-8 sustains embryonic stem cell (ESC) self-renewal and pluripotency. ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs.

In vitro activity:

As expected, levels of the CBP/β-catenin complex were significantly enhanced in ID-8-treated cells compared with control and in particular to ICG-001-treated cells. A similar result was obtained in DYRK-knockdown cells (Fig. 2I, 2J). This study concludes that ID-8, through inhibition of DYRK family members, supports maintenance of the undifferentiated state in the presence of Wnt by modulating Wnt/β-catenin signaling and enhancing the CBP/β-catenin association in hESCs, similar to the mechanism observed in mESCs treated with IQ-1 and Wnt. Reference: Stem Cells Transl Med. 2012 Jan;1(1):18-28. https://pubmed.ncbi.nlm.nih.gov/23197636/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMF	33.3	111.63
	DMF:PBS (pH 7.2) (1:2)	0.3	1.11
	DMSO	44.0	147.50
	Ethanol	2.0	6.70

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 298.30 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Monteiro MB, Ramm S, Chandrasekaran V, Boswell SA, Weber EJ, Lidberg KA, Kelly EJ, Vaidya VS. A High-Throughput Screen Identifies DYRK1A Inhibitor ID-8 that Stimulates Human Kidney Tubular Epithelial Cell Proliferation. J Am Soc Nephrol. 2018 Dec;29(12):2820-2833. doi: 10.1681/ASN.2018040392. Epub 2018 Oct 25. PMID: 30361326; PMCID: PMC6287872. 2. Hasegawa K, Yasuda SY, Teo JL, Nguyen C, McMillan M, Hsieh CL, Suemori H, Nakatsuji N, Yamamoto M, Miyabayashi T, Lutzko C, Pera MF, Kahn M. Wnt signaling orchestration with a small molecule DYRK inhibitor provides long-term xeno-free human pluripotent cell expansion. Stem Cells Transl Med. 2012 Jan;1(1):18-28. doi: 10.5966/sctm.2011-0033. Epub 2011 Dec 7. PMID: 23197636; PMCID: PMC3727690.

In vitro protocol:

In vivo protocol:

TBD

1: Monteiro MB, Ramm S, Chandrasekaran V, Boswell SA, Weber EJ, Lidberg KA, Kelly EJ, Vaidya VS. A High-Throughput Screen Identifies DYRK1A Inhibitor ID-8 that Stimulates Human Kidney Tubular Epithelial Cell Proliferation. J Am Soc Nephrol. 2018 Dec;29(12):2820-2833. doi: 10.1681/ASN.2018040392. Epub 2018 Oct 25. PMID: 30361326; PMCID: PMC6287872. 2: Sylvain V, Lafarge S, Bignon YJ. Molecular pathways involved in response to ionizing radiation of ID-8 mouse ovarian cancer cells expressing exogenous full- length Brca1 or truncated Brca1 mutant. Int J Oncol. 2001 Sep;19(3):599-607. doi: 10.3892/ijo.19.3.599. PMID: 11494042.