Polysorbate 40 | CAS#9005-66-7

MedKoo Cat#: 462696 | Name: Polysorbate 40

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Polysorbate 40 is a class of emulsifiers used in some pharmaceuticals and food preparation. It is often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from ethoxylated sorbitan esterified with fatty acids.

Chemical Structure

Polysorbate 40

CAS#9005-66-7

Theoretical Analysis

MedKoo Cat#: 462696

Name: Polysorbate 40

CAS#: 9005-66-7

Chemical Formula: C26H50O10

Exact Mass: 522.3404

Molecular Weight: 522.68

Elemental Analysis: C, 59.75; H, 9.64; O, 30.61

Price and Availability

Size	Price	Availability	Quantity
2g	USD 750.00	2 Weeks

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Synonym

Tween 40; Polysorbate 40;

IUPAC/Chemical Name

2-((R)-2-((2R,3R,4R)-3,4-bis(2-hydroxyethoxy)tetrahydrofuran-2-yl)-2-(2-hydroxyethoxy)ethoxy)ethyl dodecanoate

InChi Key

HMFKFHLTUCJZJO-OQUNMALSSA-N

InChi Code

InChI=1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3/t22-,23-,25-,26-/m1/s1

SMILES Code

CCCCCCCCCCCC(OCCOC[C@H]([C@H]1OC[C@H]([C@H]1OCCO)OCCO)OCCO)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

Polysorbate 40 is a class of emulsifiers used in some pharmaceuticals and food preparation.

In vitro activity:

This study demonstrates that tween 40, a surfactant promoter of the production of glutamate and arginine, improves L-ornithine production titers in engineered C. glutamicum S9114. The intracellular metabolism under tween 40 triggered fermentation conditions was explored using a quantitative proteomic approach, identifying 48 up-regulated and 132 down-regulated proteins when compared with the control. These results clearly demonstrate the positive effect of tween 40 addition on L-ornithine accumulation. Proteome analysis was performed to examine the impact of tween 40 addition on the physiological changes in C. glutamicum Orn8 and the results showed several promising modulation targets for developing L-ornithine-producing strains. Reference: Microb Cell Fact. 2020 Jan 6;19(1):2. https://pubmed.ncbi.nlm.nih.gov/31906967/

In vivo activity:

Mouse liver homogenization by Potter-Elvehjem homogenizer in combination with sucrose-cushion ultracentrifugation and PM enrichment with Tween 40 resulted in two times higher transporter protein quantity (Breast cancer resistance protein (Bcrp) 18.0 fmol/μg protein) in comparison with the PM samples isolated by extraction kit (Bcrp 9.8 fmol/μg protein). The evaluation of protein digestion protocols revealed that the most optimal protocol for PM protein digestion is with Lys-C and trypsin, in combination with trypsin enhancer and heat denaturation. Overall, quantities of Bcrp and Na+/K + ATPase proteins evaluated in mouse liver and kidney cortex by using our optimized PM isolation method, as well as, established digestion protocol were two to three times higher than previously reported and coefficient of variation (CV) for technical replicates was below 10%. Reference: J Pharm Biomed Anal. 2019 Feb 5;164:9-15. https://pubmed.ncbi.nlm.nih.gov/30339949/

Preparing Stock Solutions

The following data is based on the product molecular weight 522.68 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Jiang Y, Huang MZ, Chen XL, Zhang B. Proteome analysis guided genetic engineering of Corynebacterium glutamicum S9114 for tween 40-triggered improvement in L-ornithine production. Microb Cell Fact. 2020 Jan 6;19(1):2. doi: 10.1186/s12934-019-1272-0. PMID: 31906967; PMCID: PMC6943917. 2. Jankovskaja S, Kamiie J, Rezeli M, Gustavsson L, Sugihara Y, Miliotis T, Ruzgas T, Marko-Varga G. Optimization of sample preparation for transporter protein quantification in tissues by LC-MS/MS. J Pharm Biomed Anal. 2019 Feb 5;164:9-15. doi: 10.1016/j.jpba.2018.10.013. Epub 2018 Oct 7. PMID: 30339949. 3. Quenelle DC, Collins DJ, Marciani DJ, Kern ER. Effect of immunization with herpes simplex virus type-1 (HSV-1) glycoprotein D (gD) plus the immune enhancer GPI-0100 on infection with HSV-1 or HSV-2. Vaccine. 2006 Mar 6;24(10):1515-22. doi: 10.1016/j.vaccine.2005.10.017. Epub 2005 Oct 24. PMID: 16288820.

In vitro protocol:

In vivo protocol:

1. Jankovskaja S, Kamiie J, Rezeli M, Gustavsson L, Sugihara Y, Miliotis T, Ruzgas T, Marko-Varga G. Optimization of sample preparation for transporter protein quantification in tissues by LC-MS/MS. J Pharm Biomed Anal. 2019 Feb 5;164:9-15. doi: 10.1016/j.jpba.2018.10.013. Epub 2018 Oct 7. PMID: 30339949. 2. Quenelle DC, Collins DJ, Marciani DJ, Kern ER. Effect of immunization with herpes simplex virus type-1 (HSV-1) glycoprotein D (gD) plus the immune enhancer GPI-0100 on infection with HSV-1 or HSV-2. Vaccine. 2006 Mar 6;24(10):1515-22. doi: 10.1016/j.vaccine.2005.10.017. Epub 2005 Oct 24. PMID: 16288820.

1: Coleman L, Adams JRG, Buchanan W, Chen T, La Ragione RM, Liu LX. Non- Antibiotic Compounds Synergistically Kill Chronic Wound-Associated Bacteria and Disrupt Their Biofilms. Pharmaceutics. 2023 May 31;15(6):1633. doi: 10.3390/pharmaceutics15061633. PMID: 37376081; PMCID: PMC10302581. 2: Zeng L, Liu Y, Yuan Z, Wang Z. Formation and Physical Stability of Zanthoxylum bungeanum Essential Oil Based Nanoemulsions Co-Stabilized with Tea Saponin and Synthetic Surfactant. Molecules. 2021 Dec 9;26(24):7464. doi: 10.3390/molecules26247464. PMID: 34946544; PMCID: PMC8707892. 3: Ruan Y, Li X, You L, Chen J, Shen Y, Zhang J, Yuan Y, Kang L, Qin C, Wu C. Effect of Pharmaceutical Excipients on Intestinal Absorption of Metformin via Organic Cation-Selective Transporters. Mol Pharm. 2021 Jun 7;18(6):2198-2207. doi: 10.1021/acs.molpharmaceut.0c01104. Epub 2021 May 6. PMID: 33956455. 4: Bai J, Li T, Jia X, Chen Y, Nong L, Liu S, Xu C. Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii. An Acad Bras Cienc. 2020;92 Suppl 2:e20180838. doi: 10.1590/0001-3765202020180838. Epub 2020 Jul 27. PMID: 32725044. 5: Jiang Y, Huang MZ, Chen XL, Zhang B. Proteome analysis guided genetic engineering of Corynebacterium glutamicum S9114 for tween 40-triggered improvement in L-ornithine production. Microb Cell Fact. 2020 Jan 6;19(1):2. doi: 10.1186/s12934-019-1272-0. PMID: 31906967; PMCID: PMC6943917. 6: Peng YP, Chen TY, Wu CY, Chang YC, Chen KF. Dispersant-modified iron nanoparticles for mobility enhancement and TCE degradation: a comparison study. Environ Sci Pollut Res Int. 2019 Nov;26(33):34157-34166. doi: 10.1007/s11356-018-3739-7. Epub 2018 Nov 19. PMID: 30456616. 7: Wan Mohamad WAF, McNaughton D, Augustin MA, Buckow R. Characterisation of β-carotene partitioning in protein emulsions: Effects of pre-treatments, solid fat content and emulsifier type. Food Chem. 2018 Aug 15;257:361-367. doi: 10.1016/j.foodchem.2018.03.027. Epub 2018 Mar 9. PMID: 29622223. 8: Miastkowska M, Sikora E, Lasoń E, Garcia-Celma MJ, Escribano-Ferrer E, Solans C, Llinas M. Nano-emulsions as vehicles for topical delivery of forskolin. Acta Biochim Pol. 2017;64(4):713-718. doi: 10.18388/abp.2017_2334. Epub 2017 Dec 13. PMID: 29232418. 9: Hernández Y, Coello Y, Fratila RM, de la Fuente JM, Lionberger TA. Highly sensitive ratiometric quantification of cyanide in water with gold nanoparticles via Resonance Rayleigh Scattering. Talanta. 2017 May 15;167:51-58. doi: 10.1016/j.talanta.2017.02.006. Epub 2017 Feb 3. PMID: 28340752. 10: Poša M, Pilipović A, Bećarević M, Farkaš Z. pKa values of hyodeoxycholic and cholic acids in the binary mixed micelles sodium-hyodeoxycholate-Tween 40 and sodium-cholate-Tween 40: Thermodynamic stability of the micelle and the cooperative hydrogen bond formation with the steroid skeleton. Steroids. 2017 Jan;117:62-70. doi: 10.1016/j.steroids.2016.09.012. Epub 2016 Sep 17. PMID: 27651024. 11: Thakare M, Israel B, Garner S, Ahmed H, Elder D, Capomacchia A. Nonionic surfactant structure on the drug release, formulation and physical properties of ethylcellulose microspheres. Pharm Dev Technol. 2017 May;22(3):418-425. doi: 10.1080/10837450.2016.1221431. Epub 2016 Aug 30. PMID: 27499352. 12: Matsushima Y, Hirasawa T, Shimizu H. Enhancement of 1,5-diaminopentane production in a recombinant strain of Corynebacterium glutamicum by Tween 40 addition. J Gen Appl Microbiol. 2016;62(1):42-5. doi: 10.2323/jgam.62.42. PMID: 26923131. 13: Tan TB, Yussof NS, Abas F, Mirhosseini H, Nehdi IA, Tan CP. Forming a lutein nanodispersion via solvent displacement method: the effects of processing parameters and emulsifiers with different stabilizing mechanisms. Food Chem. 2016 Mar 1;194:416-23. doi: 10.1016/j.foodchem.2015.08.045. Epub 2015 Aug 14. PMID: 26471574. 14: Chen M, Chen X, Wan F, Zhang B, Chen J, Xiong Y. Effect of Tween 40 and DtsR1 on L-arginine overproduction in Corynebacterium crenatum. Microb Cell Fact. 2015 Aug 12;14:119. doi: 10.1186/s12934-015-0310-9. PMID: 26264811; PMCID: PMC4534012. 15: Beg S, Sharma G, Thanki K, Jain S, Katare OP, Singh B. Positively charged self-nanoemulsifying oily formulations of olmesartan medoxomil: Systematic development, in vitro, ex vivo and in vivo evaluation. Int J Pharm. 2015 Sep 30;493(1-2):466-82. doi: 10.1016/j.ijpharm.2015.07.048. Epub 2015 Jul 26. PMID: 26211900. 16: Pasquetti M, Chiavassa E, Tizzani P, Danesi P, Peano A. Agar Diffusion Procedures for Susceptibility Testing of Malassezia pachydermatis: Evaluation of Mueller-Hinton Agar Plus 2 % Glucose and 0.5 µg/ml Methylene Blue as the Test Medium. Mycopathologia. 2015 Oct;180(3-4):153-8. doi: 10.1007/s11046-015-9913-2. Epub 2015 Jul 3. PMID: 26138434. 17: Saberi AH, Zeeb B, Weiss J, McClements DJ. Tuneable stability of nanoemulsions fabricated using spontaneous emulsification by biopolymer electrostatic deposition. J Colloid Interface Sci. 2015 Oct 1;455:172-8. doi: 10.1016/j.jcis.2015.05.037. Epub 2015 May 29. PMID: 26070187. 18: Hao JH, Sun M. Purification and characterization of a cold alkaline protease from a psychrophilic Pseudomonas aeruginosa HY1215. Appl Biochem Biotechnol. 2015 Jan;175(2):715-22. doi: 10.1007/s12010-014-1315-2. Epub 2014 Oct 24. PMID: 25342263. 19: Barakat HS, Kassem MA, El-Khordagui LK, Khalafallah NM. Vancomycin-eluting niosomes: a new approach to the inhibition of staphylococcal biofilm on abiotic surfaces. AAPS PharmSciTech. 2014 Oct;15(5):1263-74. doi: 10.1208/s12249-014-0141-8. Epub 2014 Jun 4. PMID: 24895077; PMCID: PMC4179651. 20: Chang Y, McClements DJ. Optimization of orange oil nanoemulsion formation by isothermal low-energy methods: influence of the oil phase, surfactant, and temperature. J Agric Food Chem. 2014 Mar 12;62(10):2306-12. doi: 10.1021/jf500160y. Epub 2014 Mar 4. PMID: 24564878.