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MedKoo Cat#: 574316 | Name: ThioGlo1
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

ThioGlo1 is a thiol-reactive fluorescent probe. Upon reaction with a thiol group, a fluorescent adduct is formed that displays excitation/emission maxima of 384/513 nm, respectively. It also reacts with sulfite to form a fluorescent adduct with similar spectral characteristics, and interference from sulfite during thiol determination must be accounted for using secondary methods.

Chemical Structure

ThioGlo1
ThioGlo1
CAS#137350-66-4

Theoretical Analysis

MedKoo Cat#: 574316

Name: ThioGlo1

CAS#: 137350-66-4

Chemical Formula: C20H13NO7

Exact Mass: 379.0692

Molecular Weight: 379.32

Elemental Analysis: C, 63.33; H, 3.45; N, 3.69; O, 29.52

Price and Availability

Size Price Availability Quantity
10mg USD 350.00 2 Weeks
50mg USD 600.00 2 Weeks
100mg USD 900.00 2 Weeks
250mg USD 1,350.00 2 Weeks
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Related CAS #
No Data
Synonym
ThioGlo1, ThioGlo-1
IUPAC/Chemical Name
10-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)-9-methoxy-3-oxo-H-naphtho[2,1-b]pyran-2-carboxylic acid, methyl ester
InChi Key
NLQBAJWAMARDBZ-UHFFFAOYSA-N
InChi Code
InChI=1S/C20H13NO7/c1-26-14-6-4-10-3-5-13-11(9-12(19(24)27-2)20(25)28-13)17(10)18(14)21-15(22)7-8-16(21)23/h3-9H,1-2H3
SMILES Code
COC1=CC=C(C=CC(O2)=C3C=C(C(OC)=O)C2=O)C3=C1N4C(C=CC4=O)=O
Appearance
Solid powder
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 379.32 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
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Am J Obstet Gynecol. 2010 Apr;202(4):363.e1-5. doi: 10.1016/j.ajog.2010.01.034. PMID: 20350644; PMCID: PMC4594801. 9: Agarwal A, Wang M, Olofsson J, Orwar O, Weber SG. Control of the release of freely diffusing molecules in single-cell electroporation. Anal Chem. 2009 Oct 1;81(19):8001-8. doi: 10.1021/ac9010292. PMID: 19731948; PMCID: PMC2938737. 10: Zudans I, Agarwal A, Orwar O, Weber SG. Numerical calculations of single- cell electroporation with an electrolyte-filled capillary. Biophys J. 2007 May 15;92(10):3696-705. doi: 10.1529/biophysj.106.097683. Epub 2007 Mar 9. PMID: 17351001; PMCID: PMC1853140. 11: Agarwal A, Zudans I, Orwar O, Weber SG. Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary. Anal Chem. 2007 Jan 1;79(1):161-7. doi: 10.1021/ac061270o. PMID: 17194134; PMCID: PMC2529250. 12: Hasinoff BB, Wu X, Krokhin OV, Ens W, Standing KG, Nitiss JL, Sivaram T, Giorgianni A, Yang S, Jiang Y, Yalowich JC. Biochemical and proteomics approaches to characterize topoisomerase IIalpha cysteines and DNA as targets responsible for cisplatin-induced inhibition of topoisomerase IIalpha. Mol Pharmacol. 2005 Mar;67(3):937-47. doi: 10.1124/mol.104.004416. Epub 2004 Dec 15. PMID: 15602006. 13: Iakovlev AA, Guliaeva NV. Opredelenie tiolovykh grupp v tkani mozga pri pomoshchi Thioglo-1 [Using thioGlo-1 for the determination of thiols in brain tissue]. Biomed Khim. 2004 Jul-Aug;50(4):390-7. Russian. PMID: 15518186. 14: Fabisiak JP, Sedlov A, Kagan VE. Quantification of oxidative/nitrosative modification of CYS(34) in human serum albumin using a fluorescence-based SDS- PAGE assay. Antioxid Redox Signal. 2002 Oct;4(5):855-65. doi: 10.1089/152308602760599016. PMID: 12470514. 15: Kagan VE, Kuzmenko AI, Tyurina YY, Shvedova AA, Matsura T, Yalowich JC. Pro- oxidant and antioxidant mechanisms of etoposide in HL-60 cells: role of myeloperoxidase. Cancer Res. 2001 Nov 1;61(21):7777-84. PMID: 11691792. 16: Shvedova AA, Kommineni C, Jeffries BA, Castranova V, Tyurina YY, Tyurin VA, Serbinova EA, Fabisiak JP, Kagan VE. Redox cycling of phenol induces oxidative stress in human epidermal keratinocytes. J Invest Dermatol. 2000 Feb;114(2):354-64. doi: 10.1046/j.1523-1747.2000.00865.x. PMID: 10651998. 17: Hua S, Fabris D, Inesi G. Characterization of calcium, nucleotide, phosphate, and vanadate bound states by derivatization of sarcoplasmic reticulum ATPase with ThioGlo1. Biophys J. 1999 Oct;77(4):2217-25. doi: 10.1016/S0006-3495(99)77062-1. PMID: 10512841; PMCID: PMC1300502. 18: Kagan VE, Yalowich JC, Borisenko GG, Tyurina YY, Tyurin VA, Thampatty P, Fabisiak JP. Mechanism-based chemopreventive strategies against etoposide- induced acute myeloid leukemia: free radical/antioxidant approach. Mol Pharmacol. 1999 Sep;56(3):494-506. doi: 10.1124/mol.56.3.494. PMID: 10462537. 19: Storey BT, Alvarez JG, Thompson KA. Human sperm glutathione reductase activity in situ reveals limitation in the glutathione antioxidant defense system due to supply of NADPH. Mol Reprod Dev. 1998 Apr;49(4):400-7. doi: 10.1002/(SICI)1098-2795(199804)49:4<400::AID-MRD7>3.0.CO;2-R. PMID: 9508091.