Chlorophenol red β-D-galactopyranoside (CPRG) is a colorimetric substrate for β-galactosidase. Upon enzymatic cleavage by β-galactosidase, chlorophenol red is released, which can be quantified by colorimetric detection at 570 nm as a measure of β-galactosidase activity.
MedKoo Cat#: 462144
Name: Chlorophenol Red β-D-Galactopyranoside
CAS#: 99792-79-7
Chemical Formula: C25H22Cl2O10S
Exact Mass: 584.0311
Molecular Weight: 585.40
Elemental Analysis: C, 51.29; H, 3.79; Cl, 12.11; O, 27.33; S, 5.48
The following data is based on the product molecular weight 585.40 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |