MedKoo Cat#: 462144 | Name: Chlorophenol Red β-D-Galactopyranoside
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Chlorophenol red β-D-galactopyranoside (CPRG) is a colorimetric substrate for β-galactosidase. Upon enzymatic cleavage by β-galactosidase, chlorophenol red is released, which can be quantified by colorimetric detection at 570 nm as a measure of β-galactosidase activity.

Chemical Structure

Chlorophenol Red β-D-Galactopyranoside
Chlorophenol Red β-D-Galactopyranoside
CAS#99792-79-7

Theoretical Analysis

MedKoo Cat#: 462144

Name: Chlorophenol Red β-D-Galactopyranoside

CAS#: 99792-79-7

Chemical Formula: C25H22Cl2O10S

Exact Mass: 584.0311

Molecular Weight: 585.40

Elemental Analysis: C, 51.29; H, 3.79; Cl, 12.11; O, 27.33; S, 5.48

Price and Availability

Size Price Availability Quantity
50mg USD 350.00 2 Weeks
100mg USD 600.00 2 Weeks
250mg USD 950.00 2 Weeks
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Related CAS #
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Synonym
Chlorophenol Red β-D-Galactopyranoside; CPRG
IUPAC/Chemical Name
3-(3-chloro-4-(((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)phenyl)-3-(3-chloro-4-hydroxyphenyl)-3H-benzo[c][1,2]oxathiole 1,1-dioxide
InChi Key
UYYRDZGZGNYVBA-VPXCCNNISA-N
InChi Code
InChI=1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)25(14-3-1-2-4-20(14)38(33,34)37-25)13-6-8-18(16(27)10-13)35-24-23(32)22(31)21(30)19(11-28)36-24/h1-10,19,21-24,28-32H,11H2/t19-,21+,22+,23-,24-,25?/m1/s1
SMILES Code
ClC1=C(O[C@H]2[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O2)C=CC(C3(C4=CC(Cl)=C(O)C=C4)OS(C5=C3C=CC=C5)(=O)=O)=C1
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 585.40 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Sicard, C., Shek, N., White, D., et al. A rapid and sensitive fluorimetric β-galactosidase assay for coliform detection using chlorophenol red-β-ᴅ-galactopyranoside. Anal. Bioanal. Chem. 406(22), 5395-5403 (2014). 2: Jendresen, C., Daws, M.R., and Nilsson, L.N.G. An improved CPRG colorimetric ligand-receptor signal transduction assay based on beta-galactosidase activity in mammalian BWZ-reporter cells. J. Pharmacol. Toxicol. Methods 90, 67-75 (2018).