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MedKoo Cat#: 555752 | Name: ML329
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

ML-329 is an inhibitor of the microphthalmia-associated transcription factor (MITF) pathway with an IC50 value of 1.2 μM for promoter activity of the MITF target gene TRPM-1. ML329 showed specific activity against the MITF-dependent cells, primary melanocytes but no effect on the viability in A375 cells. ML329 reduced the expression of multiple MITF target genes, including pigment-related genes and the cell cycle regulator CDK2. As a tool compound, ML329 will be useful in elucidating the role of MITF in melanocyte lineage development and in melanoma disease progression.

Chemical Structure

ML329
ML329
CAS#19992-50-8

Theoretical Analysis

MedKoo Cat#: 555752

Name: ML329

CAS#: 19992-50-8

Chemical Formula: C16H12N2O4S

Exact Mass: 328.0518

Molecular Weight: 328.34

Elemental Analysis: C, 58.53; H, 3.68; N, 8.53; O, 19.49; S, 9.76

Price and Availability

Size Price Availability Quantity
50mg USD 450.00 2 Weeks
100mg USD 750.00 2 Weeks
200mg USD 1,250.00 2 Weeks
500mg USD 2,650.00 2 Weeks
1g USD 3,850.00 2 Weeks
2g USD 6,450.00 2 Weeks
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Related CAS #
No Data
Synonym
ML329; ML 329; ML-329; CID 12387471; CID12387471; CID-12387471;
IUPAC/Chemical Name
4-[(1,4-dihydro-1,4-dioxo-2-naphthalenyl)amino]-benzenesulfonamide
InChi Key
CSYFFQVEQXEIAB-UHFFFAOYSA-N
InChi Code
InChI=1S/C16H12N2O4S/c17-23(21,22)11-7-5-10(6-8-11)18-14-9-15(19)12-3-1-2-4-13(12)16(14)20/h1-9,18H,(H2,17,21,22)
SMILES Code
O=S(C1=CC=C(NC(C2=O)=CC(C3=C2C=CC=C3)=O)C=C1)(N)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Micropthalmia-associated transcription factor (MITF) is a lineage restricted basic helix-loop-helix leucine zipper transcription factor that is essential for melanocyte development, function and survival. 15% of human melanomas have MITF gene amplification. In addition, a vast majority of melanomas are dependent upon MITF for survival.
Product Data
Product Data
Instruction
View handling instruction
Biological target:
ML329 is a micropthalmia-associated transcription factor (MITF) inhibitor, which inhibits TRPM-1 promoter activity with an IC50 of 1.2 μM.
In vitro activity:
ML329 was tested in two MITF-dependent melanoma cell viability assays, SK-MEL-5 and MALME-3M plus a MITF-independent cell line, A375. ML329 showed specific activity against the MITF-dependent cells, primary melanocytes but no effect on the viability in A375 cells. ML329 reduced the expression of multiple MITF target genes, including pigment-related genes and the cell cycle regulator CDK2. Reference: Probe Reports from the NIH Molecular Libraries Program [Internet]. 2012 Dec 13 [updated 2014 Sep 18]. https://pubmed.ncbi.nlm.nih.gov/24027801/
In vivo activity:
The effect of potential probiotics derived from breast milk on development of intrauterine growth retardation (IUGR) newborn rats' intestine was investigated. The proliferation and differentiation stimulating effects of ML-329 and ML-446 on IECs in the jejunum, ileum, and colon were mediated by activating the Wnt pathway with increased expression of wnt, lrp5, and β-catenin genes and accumulation of β-catenin, and by downregulating the Notch signalling pathway with decreased expression of the activated notch protein. Reference: J Appl Microbiol. 2022 Aug;133(2):503-514. https://pubmed.ncbi.nlm.nih.gov/35429125/
Solvent mg/mL mM comments
Solubility
DMF 12.5 38.07
DMSO 30.3 92.38
DMSO:PBS (pH 7.2) (1:1) 0.5 1.52
Ethanol 0.1 0.30
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 328.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Proaño-Pérez E, Ollé L, Guo Y, Aparicio C, Guerrero M, Muñoz-Cano R, Martin M. MITF Downregulation Induces Death in Human Mast Cell Leukemia Cells and Impairs IgE-Dependent Degranulation. Int J Mol Sci. 2023 Feb 9;24(4):3515. doi: 10.3390/ijms24043515. PMID: 36834926; PMCID: PMC9961600. 2. Faloon PW, Bennion M, Weiner WS, Smith RA, Wurst J, Weiwer M, Hartland C, Mosher CM, Johnston S, Porubsky P, Neuenswander B, Dandapani S, Munoz B, Schoenen FJ, Metkar S, Haq R, Fisher DE, Aubé J, Palmer M, Schreiber SL. A Small Molecule Inhibitor of the MITF Molecular Pathway. 2012 Dec 13 [updated 2014 Sep 18]. In: Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010–. PMID: 24027801. 3. Lyu L, Zhou X, Zhang M, Liu L, Liu T, Niu H, Wu Y, Liang C, Han X, Zhang L. Lactobacillus derived from breast milk facilitates intestinal development in IUGR rats. J Appl Microbiol. 2022 Aug;133(2):503-514. doi: 10.1111/jam.15576. Epub 2022 Apr 27. PMID: 35429125.
In vitro protocol:
1. Proaño-Pérez E, Ollé L, Guo Y, Aparicio C, Guerrero M, Muñoz-Cano R, Martin M. MITF Downregulation Induces Death in Human Mast Cell Leukemia Cells and Impairs IgE-Dependent Degranulation. Int J Mol Sci. 2023 Feb 9;24(4):3515. doi: 10.3390/ijms24043515. PMID: 36834926; PMCID: PMC9961600. 2. Faloon PW, Bennion M, Weiner WS, Smith RA, Wurst J, Weiwer M, Hartland C, Mosher CM, Johnston S, Porubsky P, Neuenswander B, Dandapani S, Munoz B, Schoenen FJ, Metkar S, Haq R, Fisher DE, Aubé J, Palmer M, Schreiber SL. A Small Molecule Inhibitor of the MITF Molecular Pathway. 2012 Dec 13 [updated 2014 Sep 18]. In: Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010–. PMID: 24027801.
In vivo protocol:
1. Lyu L, Zhou X, Zhang M, Liu L, Liu T, Niu H, Wu Y, Liang C, Han X, Zhang L. Lactobacillus derived from breast milk facilitates intestinal development in IUGR rats. J Appl Microbiol. 2022 Aug;133(2):503-514. doi: 10.1111/jam.15576. Epub 2022 Apr 27. PMID: 35429125.
Faloon PW, Bennion M, Weiner WS, et al. A Small Molecule Inhibitor of the MITF Molecular Pathway. In: Probe Reports from the NIH Molecular Libraries Program. Bethesda (MD): National Center for Biotechnology Information (US); 2010.

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