p-APMSF | CAS# 74938-88-8 | Serine protease inhibitor

MedKoo Cat#: 574257 | Name: p-APMSF

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

p-APMSF is an irreversible inhibitor of serine proteases. p-APMSF was shown to inhibit cellular encapsulation of immulectin-coated agarose beads.

Chemical Structure

p-APMSF

CAS#74938-88-8

Theoretical Analysis

MedKoo Cat#: 574257

Name: p-APMSF

CAS#: 74938-88-8

Chemical Formula: C8H10ClFN2O2S

Exact Mass: 252.0136

Molecular Weight: 252.69

Elemental Analysis: C, 38.03; H, 3.99; Cl, 14.03; F, 7.52; N, 11.09; O, 12.66; S, 12.69

Price and Availability

Size	Price	Availability	Quantity
1mg	USD 400.00
5mg	USD 600.00
10mg	USD 800.00
25mg	USD 1,000.00

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Synonym

p-APMSF, para-APMSF, (p-Amidinophenyl)methanesulfonyl Fluoride

IUPAC/Chemical Name

4-(aminoiminomethyl)-benzenemethanesulfonyl fluoride, monohydrochloride

InChi Key

KHLLRHIUKOJXLL-UHFFFAOYSA-N

InChi Code

InChI=1S/C8H9FN2O2S.ClH/c9-14(12,13)5-6-1-3-7(4-2-6)8(10)11;/h1-4H,5H2,(H3,10,11);1H

SMILES Code

FS(CC1=CC=C(C(N)=N)C=C1)(=O)=O.Cl

Appearance

Solid powder

Purity

>95% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

p-APMSF is an irreversible inhibitor of serine proteases.

In vitro activity:

the Ki of p-APMSF for all of the above-mentioned proteases is between 1 and 2 microM. In contrast, p-APMSF in large molar excess does not inactivate chymotrypsin or acetylcholinesterase. The unique reactivity of p-APMSF has been further shown in comparison with the related compound p-nitrophenyl (p-amidinophenyl)methanesulfonate which is an active-site titrant for thrombin but reacts poorly with Factor Xa, C1-r, and C1-s and is not hydrolyzed by bovine trypsin or human plasmin. Studies with bovine trypsin have demonstrated that the inhibitory activity of p-APMSF is the result of its interaction with the diisopropyl fluorophosphate reactive site. Reference: Biochemistry. 1980 Oct 14;19(21):4859-64. https://pubmed.ncbi.nlm.nih.gov/6775696/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMSO	50.0	197.87
	Water	25.0	98.94

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 252.69 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

Laura R, Robison DJ, Bing DH. (p-Amidinophenyl)methanesulfonyl fluoride, an irreversible inhibitor of serine proteases. Biochemistry. 1980 Oct 14;19(21):4859-64. doi: 10.1021/bi00562a024. PMID: 6775696.

In vitro protocol:

In vivo protocol:

TBD

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