Phosphoramidite for the synthesis of alkyne-modified oligonucleotides. Oligonucleotides can be used for Click Chemistry modification (see the protocol). Diluent for this amidite is acetonitrile, 5 min coupling time is recommended. Standard deprotection conditions can be used for oligonucleotides.
MedKoo Cat#: 558940
Name: Alkyne Amidite, hydroxyprolinol
CAS#: 1357289-02-1
Chemical Formula: C41H52N3O6P
Exact Mass: 713.3594
Molecular Weight: 713.85
Elemental Analysis: C, 68.98; H, 7.34; N, 5.89; O, 13.45; P, 4.34
The following data is based on the product molecular weight 713.85 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |