EdU (ethynyldeoxyuridine) is a nucleoside, an analog of thymidine, carrying an ethynyl group. This nucleoside behaves as a substrate for cellular DNA replication machinery. Then, DNA containing ethynyl groups can be developed by Click chemistry reaction with various dye azides. Fluorescent DNA can be detected by microscopy, or cells can be sorted by FACS.
4910.jpg "EdU (5-ethynyl-2'-deoxyuridine)")
MedKoo Cat#: 558934
Name: EdU (5-ethynyl-2'-deoxyuridine)
CAS#: 61135-33-9
Chemical Formula: C11H12N2O5
Exact Mass: 252.0746
Molecular Weight: 252.23
Elemental Analysis: C, 52.38; H, 4.80; N, 11.11; O, 31.72
The following data is based on the product molecular weight 252.23 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
