INCA-6 | CAS#3519-82-2

MedKoo Cat#: 533366 | Name: INCA-6

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

INCA-6 is an inhibitor of interaction between calcineurin and its substrate nuclear factor of activated T cells (NFAT),

Chemical Structure

INCA-6

CAS#3519-82-2

Theoretical Analysis

MedKoo Cat#: 533366

Name: INCA-6

CAS#: 3519-82-2

Chemical Formula: C20H12O2

Exact Mass: 284.0837

Molecular Weight: 284.31

Elemental Analysis: C, 84.49; H, 4.25; O, 11.25

Price and Availability

Size	Price	Availability	Quantity
10mg	USD 320.00	2 Weeks
25mg	USD 650.00	2 Weeks

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Related CAS #

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Synonym

INCA-6; INCA 6; INCA6

IUPAC/Chemical Name

Pentacyclo[6.6.6.02,7.09,14.015,20]icosa-2(7),4,9,11,13,15,17,19-octaene-3,6-dione

InChi Key

GCHPUOHXXCNSQL-UHFFFAOYSA-N

InChi Code

InChI=1S/C20H12O2/c21-15-9-10-16(22)20-18-12-6-2-1-5-11(12)17(19(15)20)13-7-3-4-8-14(13)18/h1-10,17-18H

SMILES Code

O=C(C=C1)C(C2C3=CC=CC=C3C4C5=CC=CC=C25)=C4C1=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

INCA-6 (Triptycene-1,4-quinone) is a cell-permeable NFAT inhibitor. INCA-6 specifically blocks targeting of NFAT(P) substrate to the calcineurin (CN) phosphatase site and is an effective inhibitor of CN-NFAT signaling.

In vitro activity:

INCA-6, which inhibits NFAT translocation into nuclei, suppressed the increase in osteocyte apoptosis in the compressive force-loaded parietal bones. Reference: JBMR Plus. 2020 May 26;4(7):e10369. https://pubmed.ncbi.nlm.nih.gov/32666020/

In vivo activity:

Lastly, as a proof of concept that NFAT-inhibition may be a viable chemotherapeutic strategy to address TNFα-induced retinal leukostasis, the effect of non-isoform-specific CN/NFAT inhibition was evaluated in vivo using the broad-spectrum NFAT inhibitor INCA-6. Intravitreal injection of TNFα (50 ng/ml) significantly induced retinal leukocyte adhesion compared to control after 6 hrs (p = 0.0023, Fig. 4), and co-injection of INCA-6 (25 μM) reduced this effect by 79.2% (p = 0.0089). Reference: Sci Rep. 2015 Nov 3;5:14963. https://pubmed.ncbi.nlm.nih.gov/26527057/

	Solvent	mg/mL	mM
Solubility
	DMF	20.0	70.34
	DMSO	3.9	13.52

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 284.31 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Sogi C, Takeshita N, Jiang W, Kim S, Maeda T, Yoshida M, Oyanagi T, Ito A, Kimura S, Seki D, Takano I, Sakai Y, Fujiwara I, Kure S, Takano-Yamamoto T. Methionine Enkephalin Suppresses Osteocyte Apoptosis Induced by Compressive Force through Regulation of Nuclear Translocation of NFATc1. JBMR Plus. 2020 May 26;4(7):e10369. doi: 10.1002/jbm4.10369. PMID: 32666020; PMCID: PMC7340448. 2. Savage SR, Bretz CA, Penn JS. RNA-Seq reveals a role for NFAT-signaling in human retinal microvascular endothelial cells treated with TNFα. PLoS One. 2015 Jan 24;10(1):e0116941. doi: 10.1371/journal.pone.0116941. PMID: 25617622; PMCID: PMC4305319. 3. Bretz CA, Savage SR, Capozzi ME, Suarez S, Penn JS. NFAT isoforms play distinct roles in TNFα-induced retinal leukostasis. Sci Rep. 2015 Nov 3;5:14963. doi: 10.1038/srep14963. PMID: 26527057; PMCID: PMC4630625.

In vitro protocol:

In vivo protocol:

1. Bretz CA, Savage SR, Capozzi ME, Suarez S, Penn JS. NFAT isoforms play distinct roles in TNFα-induced retinal leukostasis. Sci Rep. 2015 Nov 3;5:14963. doi: 10.1038/srep14963. PMID: 26527057; PMCID: PMC4630625.