LysoFP-NH2 | CAS# 69408-85-1 | Fluorescent Probe

MedKoo Cat#: 574185 | Name: LysoFP-NH2

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

LysoFP-NH2 is the fluorescent form of the lysosomal turn-on fluorescent probe for carbon monoxide (CO) lysoFP-NO2. LysoFP-NH2 is formed when lysoFP-NO2 reacts with CO. It displays excitation/emission maxima of 440/528 nm, respectively.

Chemical Structure

LysoFP-NH2

CAS#69408-85-1

Theoretical Analysis

MedKoo Cat#: 574185

Name: LysoFP-NH2

CAS#: 69408-85-1

Chemical Formula: C18H19N3O3

Exact Mass: 325.1426

Molecular Weight: 325.37

Elemental Analysis: C, 66.45; H, 5.89; N, 12.91; O, 14.75

Price and Availability

Size	Price	Availability	Quantity
5mg	USD 350.00	2 weeks
10mg	USD 650.00	2 weeks

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Related CAS #

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Synonym

LysoFP-NH2

IUPAC/Chemical Name

5-amino-2-[2-(4-morpholinyl)ethyl]-1H-benz[de]isoquinoline-1,3(2H)-dione

InChi Key

JYKPDVFVKOXSSF-UHFFFAOYSA-N

InChi Code

InChI=1S/C18H19N3O3/c19-13-10-12-2-1-3-14-16(12)15(11-13)18(23)21(17(14)22)5-4-20-6-8-24-9-7-20/h1-3,10-11H,4-9,19H2

SMILES Code

NC1=CC(C2=O)=C3C(C(N2CCN4CCOCC4)=O)=CC=CC3=C1

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Emission: 528 nm Excitation: 440 nm

Product Data

Product Data

Instruction

View handling instruction

Biological target:

LysoFP-NH2 is the fluorescent form of the lysosomal turn-on fluorescent probe for carbon monoxide (CO) lysoFP-NO2.

In vitro activity:

A lysosome-targetable fluorogenic probe, LysoFP-NO2, was designed and synthesized based on a naphthalimide fluorophore that can detect selectively carbon monoxide (CO) in HEPES buffer (pH 7.4, 37 °C) through the transformation of the nitro group into an amino-functionalized system in the presence of CO. LysoFP-NO2 triggered a "turn-on" fluorescence response to CO with a simultaneous increase of fluorescence intensity by more than 75 times. Reference: Anal Chem. 2018 Feb 20;90(4):2933-2938. https://pubmed.ncbi.nlm.nih.gov/29353475/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMF	20.0	61.47
	DMSO	20.0	61.47
	DMSO:PBS (pH 7.2) (1:30)	0.0	0.09
	Ethanol	1.0	3.07

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 325.37 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Dhara K, Lohar S, Patra A, Roy P, Saha SK, Sadhukhan GC, Chattopadhyay P. A New Lysosome-Targetable Turn-On Fluorogenic Probe for Carbon Monoxide Imaging in Living Cells. Anal Chem. 2018 Feb 20;90(4):2933-2938. doi: 10.1021/acs.analchem.7b05331. Epub 2018 Feb 1. PMID: 29353475.

In vitro protocol:

In vivo protocol:

TBD

1. Dhara, K., Lohar, S., Patra, A., et al. A new lysosome-targetable turn-on fluorogenic probe for carbon monoxide imaging in living cells. Anal. Chem. 90(4), 2933-2938 (2018).