Pyrene is a polyaromatic hydrocarbon which exhibits fluorescence in blue range of the spectrum. When two pyrene residues are closely spaced, excimer fluorescence is observed at longer wavelength. Therefore, pyrene is useful as a proximity label. Pyrene exhibits affinity to graphite, and can be used as an anchor group for graphite. This reagent can be conjugated with biomolecules for their subsequent immobilization on graphite surfaces. This NHS ester allows to label biomolecules with amino groups, such as proteins, peptides, amino-DNA and other molecules. Pyrene is also a FRET donor for perylene.
MedKoo Cat#: 558786
Name: Pyrenebutyric acid NHS ester
CAS#: 114932-60-4
Chemical Formula: C24H19NO4
Exact Mass: 385.1314
Molecular Weight: 385.42
Elemental Analysis: C, 74.79; H, 4.97; N, 3.63; O, 16.60
The following data is based on the product molecular weight 385.42 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
