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MedKoo Cat#: 558775 | Name: AF488 NHS ester
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

AF488 is a bright and photostable dye, equivalent of Alexa Fluor® 488. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy. This NHS ester is an amine reactive dye, it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

Chemical Structure

AF488 NHS ester
CAS#1374019-99-4 (inner salt)

Theoretical Analysis

MedKoo Cat#: 558775

Name: AF488 NHS ester

CAS#: 1374019-99-4 (inner salt)

Chemical Formula: C37H46N5O13S2

Exact Mass: 0.0000

Molecular Weight: 832.92

Elemental Analysis: C, 53.36; H, 5.57; N, 8.41; O, 24.97; S, 7.70

Price and Availability

Size Price Availability Quantity
1mg USD 350.00 2 Weeks
5mg USD 750.00 2 Weeks
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Related CAS #
1025894-97-6 (free) 1374019-99-4 (inner salt) 1006592-46-6 (lithium)
Synonym
AF488 NHS ester; AF-488 NHS ester; AF 488 NHS ester; AlexaFluor 488 Succinimidyl Ester;
IUPAC/Chemical Name
N/A
InChi Key
WHNVHKUBVJPKDV-UHFFFAOYSA-M
InChi Code
InChI=1S/C25H17N3O13S2.2C6H15N/c26-15-5-3-12-19(11-2-1-10(9-14(11)24(31)32)25(33)41-28-17(29)7-8-18(28)30)13-4-6-16(27)23(43(37,38)39)21(13)40-20(12)22(15)42(34,35)36;2*1-4-7(5-2)6-3/h1-6,9,26H,7-8,27H2,(H,31,32)(H,34,35,36)(H,37,38,39);2*4-6H2,1-3H3/q+1;;/p-1
SMILES Code
NC1=CC=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C([O-])=O)=C4C=C5)C(OC4=C(S(=O)([O-])=O)C5=[NH+])=C1S(=O)([O-])=O.CC[NH+](CC)CC.CC[NH+](CC)CC
Appearance
Solid powder
Purity
>80% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 832.92 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
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