Synonym
TH257; TH-257; TH 257;
IUPAC/Chemical Name
N-Butyl-4-[(phenylamino)sulfonyl]-N-(phenylmethyl)benzamide
InChi Key
VNCIWNGCMAKKEO-UHFFFAOYSA-N
InChi Code
InChI=1S/C24H26N2O3S/c1-2-3-18-26(19-20-10-6-4-7-11-20)24(27)21-14-16-23(17-15-21)30(28,29)25-22-12-8-5-9-13-22/h4-17,25H,2-3,18-19H2,1H3
SMILES Code
O=C(N(CCCC)CC1=CC=CC=C1)C2=CC=C(S(=O)(NC3=CC=CC=C3)=O)C=C2
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Biological target:
TH-257 is a potent inhibitor of LIMK1 and LIMK2 with IC50 values of 84 nM and 39 nM for LIMK1 and LIMK2, respectively, and it can be used as a chemical probe for LIMK1 and LIMK2.
|
Solvent |
mg/mL |
mM |
| Solubility |
| DMSO |
42.3 |
100.00 |
| Ethanol |
42.3 |
100.00 |
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
422.54
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
Formulation protocol:
TBD
1: Hanke T, Mathea S, Woortman J, Salah E, Berger BT, Tumber A, Kashima R, Hata
A, Kuster B, Müller S, Knapp S. Development and Characterization of Type I, Type
II, and Type III LIM-Kinase Chemical Probes. J Med Chem. 2022 Oct
13;65(19):13264-13287. doi: 10.1021/acs.jmedchem.2c01106. Epub 2022 Sep 22.
PMID: 36136092; PMCID: PMC9675044.
2: Collins R, Lee H, Jones DH, Elkins JM, Gillespie JA, Thomas C, Baldwin AG,
Jones K, Waters L, Paine M, Atack JR, Ward SE, Grubisha O, Foley DW. Comparative
Analysis of Small-Molecule LIMK1/2 Inhibitors: Chemical Synthesis, Biochemistry,
and Cellular Activity. J Med Chem. 2022 Oct 27;65(20):13705-13713. doi:
10.1021/acs.jmedchem.2c00751. Epub 2022 Oct 7. PMID: 36205722; PMCID:
PMC9619402.
