MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 558691 | Name: m6A
Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

m6A, also known as N6-Methyladenosine, and 6-Methylaminopurinosine, is an endogenous urinary nucleoside product of the degradation of transfer ribonucleic acid (tRNA).

Chemical Structure

m6A
m6A
CAS#1867-73-8

Theoretical Analysis

MedKoo Cat#: 558691

Name: m6A

CAS#: 1867-73-8

Chemical Formula: C11H15N5O4

Exact Mass: 281.1124

Molecular Weight: 281.27

Elemental Analysis: C, 46.97; H, 5.38; N, 24.90; O, 22.75

Price and Availability

Size Price Availability Quantity
250mg USD 250.00 2 Weeks
1g USD 550.00 2 Weeks
5g USD 950.00 2 Weeks
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
m6A; N6-Methyladenosine; 6-Methylaminopurinosine
IUPAC/Chemical Name
(2R,3S,4R,5R)-2-(Hydroxymethyl)-5-[6-(methylamino)purin-9-yl]oxolane-3,4-diol
InChi Key
VQAYFKKCNSOZKM-IOSLPCCCSA-N
InChi Code
InChI=1S/C11H15N5O4/c1-12-9-6-10(14-3-13-9)16(4-15-6)11-8(19)7(18)5(2-17)20-11/h3-5,7-8,11,17-19H,2H2,1H3,(H,12,13,14)/t5-,7-,8-,11-/m1/s1
SMILES Code
O[C@@H]1[C@@H](CO)O[C@@H](N2C=NC3=C(NC)N=CN=C23)[C@@H]1O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
N6-Methyladenosine is the most prevalent internal (non-cap) modification present in the messenger RNA (mRNA) of all higher eukaryotes. N6-Methyladenosine can modifies viral RNAs and has antiviral activities.
In vitro activity:
Here, based on this study’s observation that m(6)A interferes with A-T/U pairing, this study reports a microarray-based technology to map m(6)A sites in mouse embryonic stem cells. This study identified 72 unbiased sites exhibiting high m(6)A levels from 66 PolyA RNAs. Bioinformatics analyses suggest identified sites are enriched on developmental regulators and may in some contexts modulate microRNA/mRNA interactions. Reference: RNA. 2015 Aug;21(8):1511-8. https://pubmed.ncbi.nlm.nih.gov/26092943/
In vivo activity:
This study demonstrated that cytokine CCL3, which is secreted by hepatocytes, promotes tumor metastasis by regulating m6A modification via vir-like m6A methyltransferase associated (VIRMA) in ICC cells. Stable cells with modified VIRMA expression were subcutaneously injected into the BABL/c nude mice. VIRMA knockdown (HuCCT1-shVIRMA) led to significantly low tumor growth rate and tumor weight compared with the other groups (HuCCT1: 0.75 ± 0.05 g, HuCCT1-shNC: 0.77 ± 0.02 g, HuCCT1-shVIRMA: 0.28 ± 0.01 g). Reference: J Transl Med. 2023 Jan 23;21(1):43. https://pubmed.ncbi.nlm.nih.gov/36691046/
Solvent mg/mL mM
Solubility
DMSO 43.5 154.65
Ethanol 13.0 46.22
Water 16.8 59.66
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 281.27 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Li Y, Wang Y, Zhang Z, Zamudio AV, Zhao JC. Genome-wide detection of high abundance N6-methyladenosine sites by microarray. RNA. 2015 Aug;21(8):1511-8. doi: 10.1261/rna.051474.115. Epub 2015 Jun 19. PMID: 26092943; PMCID: PMC4509940. 2. Wang X, Lu Z, Gomez A, Hon GC, Yue Y, Han D, Fu Y, Parisien M, Dai Q, Jia G, Ren B, Pan T, He C. N6-methyladenosine-dependent regulation of messenger RNA stability. Nature. 2014 Jan 2;505(7481):117-20. doi: 10.1038/nature12730. Epub 2013 Nov 27. PMID: 24284625; PMCID: PMC3877715. 3. Zhou S, Yang K, Chen S, Lian G, Huang Y, Yao H, Zhao Y, Huang K, Yin D, Lin H, Li Y. CCL3 secreted by hepatocytes promotes the metastasis of intrahepatic cholangiocarcinoma by VIRMA-mediated N6-methyladenosine (m6A) modification. J Transl Med. 2023 Jan 23;21(1):43. doi: 10.1186/s12967-023-03897-y. PMID: 36691046; PMCID: PMC9869516. 4. He X, Zhang J, Guo Y, Yang X, Huang Y, Hao D. METTL3-Mediated N6-Methyladenosine Modification of lncRNA D26496 Suppresses the Proliferation and Migration of Schwann Cells after Sciatic Nerve Injury. Mol Neurobiol. 2023 Jan 19. doi: 10.1007/s12035-023-03222-0. Epub ahead of print. PMID: 36656457.
In vitro protocol:
1. Li Y, Wang Y, Zhang Z, Zamudio AV, Zhao JC. Genome-wide detection of high abundance N6-methyladenosine sites by microarray. RNA. 2015 Aug;21(8):1511-8. doi: 10.1261/rna.051474.115. Epub 2015 Jun 19. PMID: 26092943; PMCID: PMC4509940. 2. Wang X, Lu Z, Gomez A, Hon GC, Yue Y, Han D, Fu Y, Parisien M, Dai Q, Jia G, Ren B, Pan T, He C. N6-methyladenosine-dependent regulation of messenger RNA stability. Nature. 2014 Jan 2;505(7481):117-20. doi: 10.1038/nature12730. Epub 2013 Nov 27. PMID: 24284625; PMCID: PMC3877715.
In vivo protocol:
1. Zhou S, Yang K, Chen S, Lian G, Huang Y, Yao H, Zhao Y, Huang K, Yin D, Lin H, Li Y. CCL3 secreted by hepatocytes promotes the metastasis of intrahepatic cholangiocarcinoma by VIRMA-mediated N6-methyladenosine (m6A) modification. J Transl Med. 2023 Jan 23;21(1):43. doi: 10.1186/s12967-023-03897-y. PMID: 36691046; PMCID: PMC9869516. 2. He X, Zhang J, Guo Y, Yang X, Huang Y, Hao D. METTL3-Mediated N6-Methyladenosine Modification of lncRNA D26496 Suppresses the Proliferation and Migration of Schwann Cells after Sciatic Nerve Injury. Mol Neurobiol. 2023 Jan 19. doi: 10.1007/s12035-023-03222-0. Epub ahead of print. PMID: 36656457.
1: Miao Z, Zhang T, Qi Y, Song J, Han Z, Ma C. Evolution of the RNA N6-methyladenosine methylome mediated by genomic duplication. Plant Physiol. 2019 Aug 13. pii: pp.00323.2019. doi: 10.1104/pp.19.00323. [Epub ahead of print] PubMed PMID: 31409695. 2: Jin H, Ying X, Que B, Wang X, Chao Y, Zhang H, Yuan Z, Qi D, Lin S, Min W, Yang M, Ji W. N(6)-methyladenosine modification of ITGA6 mRNA promotes the development and progression of bladder cancer. EBioMedicine. 2019 Aug 10. pii: S2352-3964(19)30510-9. doi: 10.1016/j.ebiom.2019.07.068. [Epub ahead of print] PubMed PMID: 31409574. 3: Xie W, Ma LL, Xu YQ, Wang BH, Li SM. METTL3 inhibits hepatic insulin sensitivity via N6-methyladenosine modification of Fasn mRNA and promoting fatty acid metabolism. Biochem Biophys Res Commun. 2019 Aug 10. pii: S0006-291X(19)31520-7. doi: 10.1016/j.bbrc.2019.08.018. [Epub ahead of print] PubMed PMID: 31405565. 4: Miao W, Li L, Zhao Y, Dai X, Chen X, Wang Y. HSP90 inhibitors stimulate DNAJB4 protein expression through a mechanism involving N(6)-methyladenosine. Nat Commun. 2019 Aug 9;10(1):3613. doi: 10.1038/s41467-019-11552-8. PubMed PMID: 31399576; PubMed Central PMCID: PMC6688989. 5: Liu XM, Zhou J, Mao Y, Ji Q, Qian SB. Programmable RNA N(6)-methyladenosine editing by CRISPR-Cas9 conjugates. Nat Chem Biol. 2019 Sep;15(9):865-871. doi: 10.1038/s41589-019-0327-1. Epub 2019 Aug 5. PubMed PMID: 31383972. 6: Liu Y, Liu Z, Tang H, Shen Y, Gong Z, Xie N, Zhang X, Wang W, Kong W, Zhou Y, Fu Y. The N(6)-Methyladenosine (m(6)A)-Forming Enzyme METTL3 Facilitates M1 Macrophage Polarization through the Methylation of STAT1 mRNA. Am J Physiol Cell Physiol. 2019 Jul 31. doi: 10.1152/ajpcell.00212.2019. [Epub ahead of print] PubMed PMID: 31365297. 7: Wu Q, Yuan X, Han R, Zhang H, Xiu R. Epitranscriptomic mechanisms of N6-methyladenosine methylation regulating mammalian hypertension development by determined spontaneously hypertensive rats pericytes. Epigenomics. 2019 Jul 30. doi: 10.2217/epi-2019-0148. [Epub ahead of print] PubMed PMID: 31357869. 8: Xu Y, Yuan XD, Wu JJ, Chen RY, Xia L, Zhang M, Han CH, Mou S. The N6-methyladenosine mRNA methylase METTL14 promotes renal ischemic reperfusion injury via suppressing YAP1. J Cell Biochem. 2019 Jul 18. doi: 10.1002/jcb.29258. [Epub ahead of print] PubMed PMID: 31318098. 9: Huang B, Ding C, Zou Q, Wang W, Li H. Cyclophosphamide Regulates N6-Methyladenosine and m6A RNA Enzyme Levels in Human Granulosa Cells and in Ovaries of a Premature Ovarian Aging Mouse Model. Front Endocrinol (Lausanne). 2019 Jun 27;10:415. doi: 10.3389/fendo.2019.00415. eCollection 2019. PubMed PMID: 31316467; PubMed Central PMCID: PMC6610338. 10: Luo Z, Zhang Z, Tai L, Zhang L, Sun Z, Zhou L. Comprehensive analysis of differences of N(6)-methyladenosine RNA methylomes between high-fat-fed and normal mouse livers. Epigenomics. 2019 Jul 10. doi: 10.2217/epi-2019-0009. [Epub ahead of print] PubMed PMID: 31290331. 11: Arguello AE, Leach RW, Kleiner RE. In Vitro Selection with a Site-Specifically Modified RNA Library Reveals the Binding Preferences of N(6)-Methyladenosine Reader Proteins. Biochemistry. 2019 Aug 6;58(31):3386-3395. doi: 10.1021/acs.biochem.9b00485. Epub 2019 Jul 23. PubMed PMID: 31287290; PubMed Central PMCID: PMC6684389. 12: Qian JY, Gao J, Sun X, Cao MD, Shi L, Xia TS, Zhou WB, Wang S, Ding Q, Wei JF. KIAA1429 acts as an oncogenic factor in breast cancer by regulating CDK1 in an N6-methyladenosine-independent manner. Oncogene. 2019 Aug;38(33):6123-6141. doi: 10.1038/s41388-019-0861-z. Epub 2019 Jul 8. PubMed PMID: 31285549. 13: Williams GD, Gokhale NS, Horner SM. Regulation of Viral Infection by the RNA Modification N6-methyladenosine. Annu Rev Virol. 2019 Jul 5. doi: 10.1146/annurev-virology-092818-015559. [Epub ahead of print] PubMed PMID: 31283446. 14: Liu T, Yang S, Sui J, Xu SY, Cheng YP, Shen B, Zhang Y, Zhang XM, Yin LH, Pu YP, Liang GY. Dysregulated N6-methyladenosine methylation writer METTL3 contributes to the proliferation and migration of gastric cancer. J Cell Physiol. 2019 Jun 24. doi: 10.1002/jcp.28994. [Epub ahead of print] PubMed PMID: 31232471. 15: Luan MW, Chen W, Xing JF, Xiao CL, Chen Y, Xie SQ. DNA N6-Methyladenosine modification role in transmitted variations from genomic DNA to RNA in Herrania umbratica. BMC Genomics. 2019 Jun 18;20(1):508. doi: 10.1186/s12864-019-5776-0. PubMed PMID: 31215402; PubMed Central PMCID: PMC6582544. 16: Liu R, Zhao F, Wei J, Yu P, Zhang J, Wang Y, Li Z, Zhang J, Zhang X, Tian X. Determination of five nucleosides by LC-MS/MS and the application of the method to quantify N(6) -methyladenosine level in liver messenger ribonucleic acid of an acetaminophen-induced hepatotoxicity mouse model. J Sep Sci. 2019 Jun 13. doi: 10.1002/jssc.201900041. [Epub ahead of print] PubMed PMID: 31197931. 17: Fan Y, Zhang C, Zhu G. Profiling of RNA N6-methyladenosine methylation during follicle selection in chicken ovary. Poult Sci. 2019 Jun 12. pii: pez277. doi: 10.3382/ps/pez277. [Epub ahead of print] PubMed PMID: 31189182. 18: Cayir A, Barrow TM, Guo L, Byun HM. Exposure to environmental toxicants reduces global N6-methyladenosine RNA methylation and alters expression of RNA methylation modulator genes. Environ Res. 2019 Aug;175:228-234. doi: 10.1016/j.envres.2019.05.011. Epub 2019 May 14. PubMed PMID: 31146095. 19: Zhou Y, Yin Z, Hou B, Yu M, Chen R, Jin H, Jian Z. Expression profiles and prognostic significance of RNA N6-methyladenosine-related genes in patients with hepatocellular carcinoma: evidence from independent datasets. Cancer Manag Res. 2019 May 1;11:3921-3931. doi: 10.2147/CMAR.S191565. eCollection 2019. PubMed PMID: 31118805; PubMed Central PMCID: PMC6503205. 20: Zhang F, Zhang YC, Liao JY, Yu Y, Zhou YF, Feng YZ, Yang YW, Lei MQ, Bai M, Wu H, Chen YQ. The subunit of RNA N6-methyladenosine methyltransferase OsFIP regulates early degeneration of microspores in rice. PLoS Genet. 2019 May 22;15(5):e1008120. doi: 10.1371/journal.pgen.1008120. eCollection 2019 May. PubMed PMID: 31116744; PubMed Central PMCID: PMC6548400. a