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MedKoo Cat#: 573596 | Name: Ponasterone A
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Ponasterone A is an analog of 20-hydroxy ecdysone. Ecdysteroids act as inducers of ecdysone-inducible mammalian expression systems.

Chemical Structure

Ponasterone A
Ponasterone A
CAS#13408-56-5

Theoretical Analysis

MedKoo Cat#: 573596

Name: Ponasterone A

CAS#: 13408-56-5

Chemical Formula: C27H44O6

Exact Mass: 464.3138

Molecular Weight: 464.64

Elemental Analysis: C, 69.79; H, 9.55; O, 20.66

Price and Availability

Size Price Availability Quantity
1mg USD 230.00
5mg USD 475.00
10mg USD 800.00
25mg USD 1,500.00
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Related CAS #
No Data
Synonym
AI3-44686, Ponasterone A
IUPAC/Chemical Name
2beta,3beta,14,20,22-Pentahydroxy-5beta-cholest-7-en-6-one
InChi Key
PJYYBCXMCWDUAZ-JJJZTNILSA-N
InChi Code
1S/C27H44O6/c1-15(2)6-7-23(31)26(5,32)22-9-11-27(33)17-12-19(28)18-13-20(29)21(30)14-24(18,3)16(17)8-10-25(22,27)4/h12,15-16,18,20-23,29-33H,6-11,13-14H2,1-5H3/t16-,18-,20+,21-,22-,23+,24+,25+,26+,27+/m0/s1
SMILES Code
C1[C@@H]2[C@](C)(C[C@@H](O)[C@@H]1O)[C@@H]1C(=CC2=O)[C@@]2(CC[C@@H]([C@@]2(C)CC1)[C@@]([C@@H](CCC(C)C)O)(O)C)O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Certificate of Analysis
View CoA: current batch, Lot# C22M07C15
Safety Data Sheet (SDS)
View Safety Data Sheet (SDS)
Instruction
View handling instruction
Biological target:
Ponasterone A (25-Deoxyecdysterone), an ecdysteroid, has strong affinity for the ecdysone receptor.
In vitro activity:
Ponasterone A (PNA), an ecdysteroid hormone activating the PI3K/Akt pathway, was studied as a new steroid to promote SMN2 overexpression in MNs differentiated from ADMSCs. After 2 weeks of treatment, cultures supplemented with PNA showed a longer survival and a 1.2-fold increase in the expression of SMN2 (an overall 5.6-fold increase; *P ≤0.05), as confirmed by the Western blot analysis. The PNA treatment increased the levels of ChAT, Isl1, Hb9, and Nkx2 expression in MN-like cells. Reference: J Mol Neurosci. 2019 Feb;67(2):247-257. https://pubmed.ncbi.nlm.nih.gov/30535775/
In vivo activity:
TBD
Solvent mg/mL mM comments
Solubility
Ethanol:PBS (pH 7.2) (1:2) 0.3 0.65
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 464.64 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Mohseni R, Ashrafi MR, Ai J, Nikougoftar M, Mohammadi M, Ghahvechi-Akbari M, Shoae-Hassani A, Hamidieh AA. Overexpression of SMN2 Gene in Motoneuron-Like Cells Differentiated from Adipose-Derived Mesenchymal Stem Cells by Ponasterone A. J Mol Neurosci. 2019 Feb;67(2):247-257. doi: 10.1007/s12031-018-1232-x. Epub 2018 Dec 7. PMID: 30535775. 2. Downey PM, Lozza G, Petrò R, Diodato E, Foglia C, Bottazzoli F, Brusa R, Asquini T, Reggiani A, Grilli M. Ecdysone-based system for controlled inducible expression of metabotropic glutamate receptor subtypes 2, 5, and 8. J Biomol Screen. 2005 Dec;10(8):841-8. doi: 10.1177/1087057105280285. Epub 2005 Oct 18. PMID: 16234343.
In vitro protocol:
1. Mohseni R, Ashrafi MR, Ai J, Nikougoftar M, Mohammadi M, Ghahvechi-Akbari M, Shoae-Hassani A, Hamidieh AA. Overexpression of SMN2 Gene in Motoneuron-Like Cells Differentiated from Adipose-Derived Mesenchymal Stem Cells by Ponasterone A. J Mol Neurosci. 2019 Feb;67(2):247-257. doi: 10.1007/s12031-018-1232-x. Epub 2018 Dec 7. PMID: 30535775. 2. Downey PM, Lozza G, Petrò R, Diodato E, Foglia C, Bottazzoli F, Brusa R, Asquini T, Reggiani A, Grilli M. Ecdysone-based system for controlled inducible expression of metabotropic glutamate receptor subtypes 2, 5, and 8. J Biomol Screen. 2005 Dec;10(8):841-8. doi: 10.1177/1087057105280285. Epub 2005 Oct 18. PMID: 16234343.
In vivo protocol:
TBD
1: Gonsalves SE, Neal SJ, Kehoe AS, Westwood JT. Genome-wide examination of the transcriptional response to ecdysteroids 20-hydroxyecdysone and ponasterone A in Drosophila melanogaster. BMC Genomics. 2011 Sep 29;12:475. doi: 10.1186/1471-2164-12-475. PubMed PMID: 21958154; PubMed Central PMCID: PMC3228561. 2: Hori M, Suzuki K, Udono MU, Yamauchi M, Mine M, Watanabe M, Kondo S, Hozumi Y. Establishment of ponasterone A-inducible the wild-type p53 protein-expressing clones from HSC-1 cells, cell growth suppression by p53 expression and the suppression mechanism. Arch Dermatol Res. 2009 Oct;301(9):631-46. doi: 10.1007/s00403-008-0915-5. Epub 2008 Nov 14. PubMed PMID: 19009304.

View History

BLU-2864

BLU-2864