Meldola blue | CAS#7057-57-0 | Lactate measurement biosensor

MedKoo Cat#: 464300 | Name: Meldola blue

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Meldola's blue is a biosensor for the measurement of lactate in serum has been developed, which is based on a screen-printed carbon electrode, modified with Meldola's Blue-Reinecke Salt (MBRS-SPCE), coated with the enzyme lactate dehydrogenase NAD+ dependent (from Porcine heart), and NAD+.

Chemical Structure

Meldola blue

CAS#7057-57-0

Theoretical Analysis

MedKoo Cat#: 464300

Name: Meldola blue

CAS#: 7057-57-0

Chemical Formula: C18H15Cl3N2OZn

Exact Mass:

Molecular Weight: 447.06

Elemental Analysis: C, 48.36; H, 3.38; Cl, 23.79; N, 6.27; O, 3.58; Zn, 14.62

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.

Bulk Inquiry

Related CAS #

No Data

Synonym

Meldola blue;

IUPAC/Chemical Name

9-(dimethylamino)benzo[a]phenoxazin-7-ium zinc(II) chloride

InChi Key

YTEJSAFVYHDCSN-UHFFFAOYSA-K

InChi Code

InChI=1S/C18H15N2O.3ClH.Zn/c1-20(2)13-8-9-15-17(11-13)21-16-10-7-12-5-3-4-6-14(12)18(16)19-15;;;;/h3-11H,1-2H3;3*1H;/q+1;;;;+2/p-3

SMILES Code

CN(c(cc1)cc2c1nc(c(cc3)[o+]2)c4c3cccc4)C.[Cl-].[Cl-].[Cl-].[Zn+2]

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Instruction

View handling instruction

Preparing Stock Solutions

The following data is based on the product molecular weight 447.06 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

1: Ribeiro DB, Santos Silva G, Dos Santos DR, Castro Costa AR, Braga Ribeiro E, Badea M, Nunes GS. Determination of the Antioxidant Activity of Samples of Tea and Commercial Sources of Vitamin C, Using an Enzymatic Biosensor. Antioxidants (Basel). 2021 Feb 22;10(2):324. doi: 10.3390/antiox10020324. PMID: 33671686; PMCID: PMC7927098. 2: Semenova D, Pinto T, Koch M, Gernaey KV, Junicke H. Electrochemical tuning of alcohol oxidase and dehydrogenase catalysis via biosensing towards butanol-1 detection in fermentation media. Biosens Bioelectron. 2020 Dec 15;170:112702. doi: 10.1016/j.bios.2020.112702. Epub 2020 Oct 7. PMID: 33045667. 3: Titoiu AM, Necula-Petrareanu G, Visinescu D, Dinca V, Bonciu A, Mihailescu CN, Purcarea C, Boukherroub R, Szunerits S, Vasilescu A. Flow injection enzymatic biosensor for aldehydes based on a Meldola Blue-Ni complex electrochemical mediator. Mikrochim Acta. 2020 Sep 5;187(10):550. doi: 10.1007/s00604-020-04477-3. PMID: 32888083. 4: Hughes G, Pemberton RM, Fielden PR, Hart JP. A Reagentless, Screen-Printed Amperometric Biosensor for the Determination of Glutamate in Food and Clinical Applications. Methods Mol Biol. 2017;1572:1-12. doi: 10.1007/978-1-4939-6911-1_1. PMID: 28299677. 5: Hua E, Wang L, Jing X, Chen C, Xie G. One-step fabrication of integrated disposable biosensor based on ADH/NAD+/meldola's blue/graphitized mesoporous carbons/chitosan nanobiocomposite for ethanol detection. Talanta. 2013 Jul 15;111:163-9. doi: 10.1016/j.talanta.2013.02.064. Epub 2013 Mar 7. PMID: 23622540. 6: Sezgin Z, Biberoglu K, Chupakhin V, Makhaeva GF, Tacal O. Determination of binding points of methylene blue and cationic phenoxazine dyes on human butyrylcholinesterase. Arch Biochem Biophys. 2013 Apr 1;532(1):32-8. doi: 10.1016/j.abb.2013.01.003. Epub 2013 Jan 25. PMID: 23353050. 7: Hope-Roberts M, Horobin RW. Biomedical applications and chemical nature of three dyes first synthesized by Raphael Meldola: isamine blue, Meldola's blue and naphthol green B. Biotech Histochem. 2012 May;87(4):295-9. doi: 10.3109/10520295.2011.639722. Epub 2011 Dec 13. PMID: 22149360. 8: Mažeikienė R, Niaura G, Eicher-Lorka O, Malinauskas A. Raman spectroelectrochemical study of Meldola blue, adsorbed and electropolymerized at a gold electrode. J Colloid Interface Sci. 2011 May 1;357(1):189-97. doi: 10.1016/j.jcis.2011.01.086. Epub 2011 Feb 1. PMID: 21349529. 9: Smutok O, Broda D, Smutok H, Dmytruk K, Gonchar M. Chromate-reducing activity of Hansenula polymorpha recombinant cells over-producing flavocytochrome b₂. Chemosphere. 2011 Apr;83(4):449-54. doi: 10.1016/j.chemosphere.2010.12.078. Epub 2011 Feb 18. PMID: 21315405. 10: Canevari TC, Vinhas RC, Landers R, Gushikem Y. SiO2/SnO2/Sb2O5 microporous ceramic material for immobilization of Meldola's blue: application as an electrochemical sensor for NADH. Biosens Bioelectron. 2011 Jan 15;26(5):2402-6. doi: 10.1016/j.bios.2010.10.020. Epub 2010 Oct 16. PMID: 21067911. 11: Chang H, Khan R, Rong Z, Sapelkin A, Vadgama P. Study of albumin and fibrinogen membranes formed by interfacial crosslinking using microfluidic flow. Biofabrication. 2010 Sep;2(3):035002. doi: 10.1088/1758-5082/2/3/035002. Epub 2010 Sep 8. PMID: 20823505. 12: Piano M, Serban S, Pittson R, Drago GA, Hart JP. Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+. Talanta. 2010 Jun 30;82(1):34-7. doi: 10.1016/j.talanta.2010.03.051. Epub 2010 Apr 1. PMID: 20685431. 13: Piano M, Serban S, Biddle N, Pittson R, Drago GA, Hart JP. A flow injection system, comprising a biosensor based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt coated with glucose dehydrogenase, for the measurement of glucose. Anal Biochem. 2010 Jan 15;396(2):269-74. doi: 10.1016/j.ab.2009.09.028. Epub 2009 Sep 18. PMID: 19766585. 14: Arvinte A, Rotariu L, Bala C, Gurban AM. Synergistic effect of mediator- carbon nanotube composites for dehydrogenases and peroxidases based biosensors. Bioelectrochemistry. 2009 Sep;76(1-2):107-14. doi: 10.1016/j.bioelechem.2009.04.005. Epub 2009 May 3. PMID: 19467932. 15: Zhou M, Deng L, Wen D, Shang L, Jin L, Dong S. Highly ordered mesoporous carbons-based glucose/O2 biofuel cell. Biosens Bioelectron. 2009 May 15;24(9):2904-8. doi: 10.1016/j.bios.2009.02.028. Epub 2009 Mar 6. PMID: 19321330. 16: Luo P, Xie G, Liu Y, Xu H, Deng S, Song F. Electrochemical detection of blood alcohol concentration using a disposable biosensor based on screen-printed electrode modified with Nafion and gold nanoparticles. Clin Chem Lab Med. 2008;46(11):1641-7. doi: 10.1515/CCLM.2008.308. PMID: 19012530. 17: Vasilescu A, Noguer T, Andreescu S, Calas-Blanchard C, Bala C, Marty JL. Strategies for developing NADH detectors based on Meldola Blue and screen- printed electrodes: a comparative study. Talanta. 2003 Mar 10;59(4):751-65. doi: 10.1016/S0039-9140(02)00614-8. PMID: 18968963. 18: Torabi F, Ramanathan K, Larsson PO, Gorton L, Svanberg K, Okamoto Y, Danielsson B, Khayyami M. Coulometric determination of NAD+ and NADH in normal and cancer cells using LDH, RVC and a polymer mediator. Talanta. 1999 Nov 15;50(4):787-97. doi: 10.1016/s0039-9140(99)00134-4. PMID: 18967769. 19: Yücel YY, Tacal O, Ozer I. Comparative effects of cationic triarylmethane, phenoxazine and phenothiazine dyes on horse serum butyrylcholinesterase. Arch Biochem Biophys. 2008 Oct 15;478(2):201-5. doi: 10.1016/j.abb.2008.07.006. Epub 2008 Jul 16. PMID: 18656440. 20: Luo P, Liu Y, Xie G, Xiong X, Deng S, Song F. Determination of serum alcohol using a disposable biosensor. Forensic Sci Int. 2008 Aug 6;179(2-3):192-8. doi: 10.1016/j.forsciint.2008.06.002. Epub 2008 Jul 23. PMID: 18653295.