DIM-C-pPhCO2Me | CAS#151358-48-4 | NR4A1 Antagonist| MedKoo

MedKoo Cat#: 555482 | Name: DIM-C-pPhCO2Me

Description:

WARNING: This product is for research use only, not for human or veterinary use.

DIM-C-pPhCO2Me is a nuclear receptor 4A1 (NR4A1) antagonist with antineoplastic activity.

Chemical Structure

DIM-C-pPhCO2Me

CAS#151358-48-4

Theoretical Analysis

MedKoo Cat#: 555482

Name: DIM-C-pPhCO2Me

CAS#: 151358-48-4

Chemical Formula: C25H20N2O2

Exact Mass: 380.1525

Molecular Weight: 380.45

Elemental Analysis: C, 78.93; H, 5.30; N, 7.36; O, 8.41

Price and Availability

Size	Price	Availability
10mg	USD 125.00	Ready to ship
25mg	USD 250.00	Ready to ship
50mg	USD 425.00	Ready to ship
100mg	USD 750.00	Ready to ship
200mg	USD 1,250.00	Ready to ship
500mg	USD 2,450.00	Ready to ship
1g	USD 3,450.00	Ready to ship
2g	USD 5,850.00	Ready to ship

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Related CAS #

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Synonym

DIM-C-pPhCO2Me;

IUPAC/Chemical Name

Benzoic acid, 4-(di-1H-indol-3-ylmethyl)-, methyl ester

InChi Key

BBAOKSZCULLDIW-UHFFFAOYSA-N

InChi Code

InChI=1S/C25H20N2O2/c1-29-25(28)17-12-10-16(11-13-17)24(20-14-26-22-8-4-2-6-18(20)22)21-15-27-23-9-5-3-7-19(21)23/h2-15,24,26-27H,1H3

SMILES Code

O=C(OC)C1=CC=C(C(C2=CNC3=C2C=CC=C3)C4=CNC5=C4C=CC=C5)C=C1

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Certificate of Analysis

View CoA: current batch, Lot# A22T07K08

QC Data

View QC data: current batch, Lot#A22T07K08

Safety Data Sheet (SDS)

View Safety Data Sheet (SDS)

Instruction

View handling instruction

Biological target:

DIM-C-pPhCO2Me is a nuclear receptor 4A1 (NR4A1) antagonist.

In vitro activity:

This study also investigated the antimigratory effects of DIM-C-pPhCO2Me in pancreatic cancer cells after knockdown of NR4A1 or β1-integrin. DIM-C-pPhCO2Me decreased migration; however, in cells depleted of NR4A1 or β1-integrin minimum inhibition was observed in cells after treatment with DIM-C-pPhCO2Me (Fig. 4A) and similar results were observed in colon cancer cells (Fig. 4B) and also with DIM-C-pPhOH (Suppl. Fig. S2). Reference: Mol Carcinog. 2017 Sep;56(9):2066-2075. https://pubmed.ncbi.nlm.nih.gov/28418095/

In vivo activity:

This study treated BRx142-generated orthotopic tumors with the bis-indole-derived NR4A1 antagonist DIM-C-pPhCO2Me (NR4A1-i), which suppresses migration and induces cell death in breast cancer cells. Compared with vehicle, NR4A1-i caused tumor shrinkage (Figure 6E). Residual tumors in NR4A1-i-treated mice have elevated FOS expression, increased levels of the DNA damage marker γH2AX, and decreased expression of Ki-67 (Figures 6F and 6G). Reference: Mol Cell. 2021 Oct 7;81(19):4041-4058.e15. https://pubmed.ncbi.nlm.nih.gov/34624217/

	Solvent	mg/mL	mM
Solubility
	DMSO	100.5	264.16
	Ethanol	38.0	99.88

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 380.45 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Hedrick E, Lee SO, Safe S. The nuclear orphan receptor NR4A1 regulates β1-integrin expression in pancreatic and colon cancer cells and can be targeted by NR4A1 antagonists. Mol Carcinog. 2017 Sep;56(9):2066-2075. doi: 10.1002/mc.22662. Epub 2017 May 9. PMID: 28418095; PMCID: PMC5546981. 2. Lacey A, Hedrick E, Li X, Patel K, Doddapaneni R, Singh M, Safe S. Nuclear receptor 4A1 (NR4A1) as a drug target for treating rhabdomyosarcoma (RMS). Oncotarget. 2016 May 24;7(21):31257-69. doi: 10.18632/oncotarget.9112. PMID: 27144436; PMCID: PMC5058754. 3. Guo H, Golczer G, Wittner BS, Langenbucher A, Zachariah M, Dubash TD, Hong X, Comaills V, Burr R, Ebright RY, Horwitz E, Vuille JA, Hajizadeh S, Wiley DF, Reeves BA, Zhang JM, Niederhoffer KL, Lu C, Wesley B, Ho U, Nieman LT, Toner M, Vasudevan S, Zou L, Mostoslavsky R, Maheswaran S, Lawrence MS, Haber DA. NR4A1 regulates expression of immediate early genes, suppressing replication stress in cancer. Mol Cell. 2021 Oct 7;81(19):4041-4058.e15. doi: 10.1016/j.molcel.2021.09.016. PMID: 34624217; PMCID: PMC8549465.

In vitro protocol:

In vivo protocol:

1. Guo H, Golczer G, Wittner BS, Langenbucher A, Zachariah M, Dubash TD, Hong X, Comaills V, Burr R, Ebright RY, Horwitz E, Vuille JA, Hajizadeh S, Wiley DF, Reeves BA, Zhang JM, Niederhoffer KL, Lu C, Wesley B, Ho U, Nieman LT, Toner M, Vasudevan S, Zou L, Mostoslavsky R, Maheswaran S, Lawrence MS, Haber DA. NR4A1 regulates expression of immediate early genes, suppressing replication stress in cancer. Mol Cell. 2021 Oct 7;81(19):4041-4058.e15. doi: 10.1016/j.molcel.2021.09.016. PMID: 34624217; PMCID: PMC8549465.