Synonym
PIP-199; PIP199; PIP 199
IUPAC/Chemical Name
4-[(1-Hydroxy-2-phenyl-1H-indol-3-yl)-pyridin-2-yl-methyl]-piperazine-1-carboxylic acid ethyl ester
InChi Key
ROTCTSYSZSYNHB-UHFFFAOYSA-N
InChi Code
InChI=1S/C27H28N4O3/c1-2-34-27(32)30-18-16-29(17-19-30)26(22-13-8-9-15-28-22)24-21-12-6-7-14-23(21)31(33)25(24)20-10-4-3-5-11-20/h3-15,26,33H,2,16-19H2,1H3
SMILES Code
O=C(N1CCN(C(C2=C(C3=CC=CC=C3)N(O)C4=C2C=CC=C4)C5=NC=CC=C5)CC1)OCC
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
Biological target:
PIP-199 is a selective inhibitor of RMI (RecQ-mediated genome instability protein) core complex/MM2 interaction, with an IC50 of 36 μM.
|
Solvent |
mg/mL |
mM |
comments |
| Solubility |
| DMSO |
41.7 |
91.27 |
|
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
456.55
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
Wu X, Krishna Sudhakar H, Alcock LJ, Lau YH. Mannich Base PIP-199 Is a Chemically Unstable Pan-Assay Interference Compound. J Med Chem. 2023 Aug 24;66(16):11271-11281. doi: 10.1021/acs.jmedchem.3c00674. Epub 2023 Aug 9. PMID: 37555818.
Voter, A. F., Manthei, K. A., & Keck, J. L. (2016). A high-throughput screening strategy to identify protein-protein interaction inhibitors that block the Fanconi Anemia DNA repair pathway. Journal of biomolecular screening, 21(6), 626-633.
Lu, R., O’Rourke, J. J., Sobinoff, A. P., Allen, J. A., Nelson, C. B., Tomlinson, C. G., ... & Pickett, H. A. (2019). The FANCM-BLM-TOP3A-RMI complex suppresses alternative lengthening of telomeres (ALT). Nature communications, 10(1), 2252.
Yang, S. Y., Chang, E. Y., Lim, J., Kwan, H. H., Monchaud, D., Yip, S., ... & Wong, J. M. (2021). G-quadruplexes mark alternative lengthening of telomeres. NAR cancer, 3(3), zcab031.
