IUPAC/Chemical Name
2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-6-(2-(4'-(2-(4-hydroxyphenyl)diazenyl)(1,1'-biphenyl)-4-yl)diazenyl)-3-(2-(4-nitrophenyl)diazenyl)-, sodium salt (1:2)
InChi Key
VLFKFKCRUCJVNE-NQJWOQTRSA-L
InChi Code
InChI=1S/C34H24N8O10S2.2Na/c35-31-30-21(17-28(53(47,48)49)32(31)40-38-24-9-13-26(14-10-24)42(45)46)18-29(54(50,51)52)33(34(30)44)41-39-23-7-3-20(4-8-23)19-1-5-22(6-2-19)36-37-25-11-15-27(43)16-12-25;;/h1-18,43-44H,35H2,(H,47,48,49)(H,50,51,52);;/q;2*+1/p-2/b37-36+,40-38+,41-39+;;
SMILES Code
O=S(C1=C(/N=N/C2=CC=C([N+]([O-])=O)C=C2)C(N)=C3C(O)=C(/N=N/C4=CC=C(C5=CC=C(/N=N/C6=CC=C(O)C=C6)C=C5)C=C4)C(S(=O)([O-])=O)=CC3=C1)([O-])=O.[Na+].[Na+]
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
Biological target:
Direct Green 6 is a green dye.
In vitro activity:
This study evaluated the remediation of the textile dye, Direct Green 6 (DG6), by Spirulina platensis, and investigated the novel possibility that DG6 treatment enhances production of the biopolymer, polyhydroxybutyrate (PHB). This study showed that both live and dead cells of Spirulina were capable of DG6 remediation, but live cells could be re-used with no loss of remediation efficiency. Furthermore, DG6 remediation by live cells resulted in increased algal biomass and trichome lengths, and stimulated production of valuable metabolites, including PHB, antioxidants, carbohydrates and pigments (phycobilins and astaxanthin).
Reference: Chemosphere. 2021 Oct;280:130920. https://pubmed.ncbi.nlm.nih.gov/34162106/
In vivo activity:
The present paper emphasizes on the histopathological effects of an azo dye, Direct Green 6 on the kidney and gills of Labeo rohita by light microscopy.
Reference: J Environ Biol. 2013 Mar;34(2):147-52. https://pubmed.ncbi.nlm.nih.gov/24620571/
Preparing Stock Solutions
The following data is based on the
product
molecular weight
812.70
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
Formulation protocol:
1. Moradi Z, Madadkar Haghjou M, Zarei M, Colville L, Raza A. Synergy of production of value-added bioplastic, astaxanthin and phycobilin co-products and Direct Green 6 textile dye remediation in Spirulina platensis. Chemosphere. 2021 Oct;280:130920. doi: 10.1016/j.chemosphere.2021.130920. Epub 2021 May 21. PMID: 34162106.
2. Mahmoodi NM, Abdi J, Najafi F. Gemini polymeric nanoarchitecture as a novel adsorbent: synthesis and dye removal from multicomponent system. J Colloid Interface Sci. 2013 Jun 15;400:88-96. doi: 10.1016/j.jcis.2013.03.014. Epub 2013 Mar 21. PMID: 23582906.
3. Barot J, Bahadur A. Behavioural and histopathological effects of azodye on kidney and gills of Labeo rohita fingerlings. J Environ Biol. 2013 Mar;34(2):147-52. PMID: 24620571.
In vitro protocol:
1. Moradi Z, Madadkar Haghjou M, Zarei M, Colville L, Raza A. Synergy of production of value-added bioplastic, astaxanthin and phycobilin co-products and Direct Green 6 textile dye remediation in Spirulina platensis. Chemosphere. 2021 Oct;280:130920. doi: 10.1016/j.chemosphere.2021.130920. Epub 2021 May 21. PMID: 34162106.
2. Mahmoodi NM, Abdi J, Najafi F. Gemini polymeric nanoarchitecture as a novel adsorbent: synthesis and dye removal from multicomponent system. J Colloid Interface Sci. 2013 Jun 15;400:88-96. doi: 10.1016/j.jcis.2013.03.014. Epub 2013 Mar 21. PMID: 23582906.
In vivo protocol:
1. Barot J, Bahadur A. Behavioural and histopathological effects of azodye on kidney and gills of Labeo rohita fingerlings. J Environ Biol. 2013 Mar;34(2):147-52. PMID: 24620571.
1: Naraginti S, Thejaswini TV, Prabhakaran D, Sivakumar A, Satyanarayana VS, Arun Prasad AS. Enhanced photo-catalytic activity of Sr and Ag co-doped TiO2 nanoparticles for the degradation of Direct Green-6 and Reactive Blue-160 under UV & visible light. Spectrochim Acta A Mol Biomol Spectrosc. 2015 Oct 5;149:571-9. doi: 10.1016/j.saa.2015.04.101. Epub 2015 May 8. PubMed PMID: 25983059.
2: Mahmoodi NM, Arabloo M, Abdi J. Laccase immobilized manganese ferrite nanoparticle: synthesis and LSSVM intelligent modeling of decolorization. Water Res. 2014 Dec 15;67:216-26. doi: 10.1016/j.watres.2014.09.011. Epub 2014 Sep 21. PubMed PMID: 25282090.
3: Mahmoodi NM, Abdi J, Bastani D. Direct dyes removal using modified magnetic ferrite nanoparticle. J Environ Health Sci Eng. 2014 Jun 11;12:96. doi: 10.1186/2052-336X-12-96. eCollection 2014. PubMed PMID: 24991427; PubMed Central PMCID: PMC4077710.
4: Barot J, Bahadur A. Behavioural and histopathological effects of azodye on kidney and gills of Labeo rohita fingerlings. J Environ Biol. 2013 Mar;34(2):147-52. PubMed PMID: 24620571.
5: Mahmoodi NM, Soltani-Gordefaramarzi S, Sadeghi-Kiakhani M. Dye removal using modified copper ferrite nanoparticle and RSM analysis. Environ Monit Assess. 2013 Dec;185(12):10235-48. doi: 10.1007/s10661-013-3328-7. Epub 2013 Jul 13. PubMed PMID: 23852534.
6: Mahmoodi NM, Abdi J, Najafi F. Gemini polymeric nanoarchitecture as a novel adsorbent: synthesis and dye removal from multicomponent system. J Colloid Interface Sci. 2013 Jun 15;400:88-96. doi: 10.1016/j.jcis.2013.03.014. Epub 2013 Mar 21. PubMed PMID: 23582906.
7: Pazarlioglu NK, Akkaya A, Akdogan HA, Gungor B. Biodegradation of Direct Blue 15 by free and immobilized Trametes versicolor. Water Environ Res. 2010 Jul;82(7):579-85. PubMed PMID: 20669718.
