IU1-47 | CAS#670270-31-2 | USP14 inhibitor

MedKoo Cat#: 563728 | Name: IU1-47

Description:

WARNING: This product is for research use only, not for human or veterinary use.

IU1-47 is a potent and selective inhibitor of USP14.

Chemical Structure

IU1-47

CAS#670270-31-2

Theoretical Analysis

MedKoo Cat#: 563728

Name: IU1-47

CAS#: 670270-31-2

Chemical Formula: C19H23ClN2O

Exact Mass: 330.1499

Molecular Weight: 330.86

Elemental Analysis: C, 68.98; H, 7.01; Cl, 10.71; N, 8.47; O, 4.84

Price and Availability

Size	Price	Availability
50mg	USD 450.00	2 Weeks
100mg	USD 750.00	2 Weeks
200mg	USD 1,250.00	2 Weeks
500mg	USD 2,650.00	2 Weeks
1g	USD 3,850.00	2 Weeks
2g	USD 6,250.00	2 Weeks

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Related CAS #

No Data

Synonym

IU1-47; IU1 47; IU147;

IUPAC/Chemical Name

1-(1-(4-Chlorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl)-2-(piperidin-1-yl)ethanone

InChi Key

VXQIPRWKLACSKZ-UHFFFAOYSA-N

InChi Code

InChI=1S/C19H23ClN2O/c1-14-12-18(19(23)13-21-10-4-3-5-11-21)15(2)22(14)17-8-6-16(20)7-9-17/h6-9,12H,3-5,10-11,13H2,1-2H3

SMILES Code

CC1=CC(C(CN2CCCCC2)=O)=C(C)N1C3=CC=C(Cl)C=C3

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.03.00

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

IU1-47 is a potent and specific USP14 inhibitor with an IC50 of 0.6 μM. IU1-47 inhibits IsoT/USP5 with an IC50 of 20 μM.

In vitro activity:

Similarly, compared with DMSO-treated cells, the IU1-47-treated cells markedly reduced both cell viability and proliferation rate of A549 cells in a dose-dependent manner (Fig. 1d, e). These data suggest that the proliferation of A549 cells is associated with USP14 inhibition. Reference: Cell Stress Chaperones. 2020 Nov;25(6):909-917. https://pubmed.ncbi.nlm.nih.gov/32632734/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMSO	10.0	30.22

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 330.86 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Moghadami AA, Aboutalebi Vand Beilankouhi E, Kalantary-Charvadeh A, Hamzavi M, Mosayyebi B, Sedghi H, Ghorbani Haghjo A, Nazari Soltan Ahmad S. Inhibition of USP14 induces ER stress-mediated autophagy without apoptosis in lung cancer cell line A549. Cell Stress Chaperones. 2020 Nov;25(6):909-917. doi: 10.1007/s12192-020-01125-w. Epub 2020 Jul 6. PMID: 32632734; PMCID: PMC7591669. 2. Han KH, Kwak M, Lee TH, Park MS, Jeong IH, Kim MJ, Jin JO, Lee PC. USP14 Inhibition Regulates Tumorigenesis by Inducing Autophagy in Lung Cancer In Vitro. Int J Mol Sci. 2019 Oct 24;20(21):5300. doi: 10.3390/ijms20215300. PMID: 31653087; PMCID: PMC6862215.

In vitro protocol:

In vivo protocol:

TBD

1: Boselli M, Lee BH, Robert J, Prado MA, Min SW, Cheng C, Silva MC, Seong C, Elsasser S, Hatle KM, Gahman TC, Gygi SP, Haggarty SJ, Gan L, King RW, Finley D. An inhibitor of the proteasomal deubiquitinating enzyme USP14 induces tau elimination in cultured neurons. J Biol Chem. 2017 Nov 24;292(47):19209-19225. doi: 10.1074/jbc.M117.815126. Epub 2017 Sep 26. PubMed PMID: 28972160; PubMed Central PMCID: PMC5702663.